4,203 results

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pcDNA3.1-Peredox-mCherry

Plasmid

#32383

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 APEX2-NES

Plasmid

#49386

Purpose

Expresses APEX2 in the cytosol of mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Flag - APEX2 - NES

Tags

Flag

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available Since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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perbB2-EGFP

Plasmid

#39321

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB2 (ERBB2 Human)

Tags

egfp

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1-GFP

Plasmid

#51407

Purpose

Is a biosensor for PI(4,5)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62

Plasmid

#38277

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC control

Plasmid

#10879

Purpose

Negative control shRNA vector containing non-hairpin insert.

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Insert

non-hairpin 18bp

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8s

Plasmid

#162371

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CMV

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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Apple-53BP1trunc

Plasmid

#69531

Purpose

Fluorescent reporter for DNA damage

Depositor

Ralph Weissleder

Article

Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

Apple fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-XBP1s

Plasmid

#63680

Purpose

Mammalian expression of Flag tagged XBP1s

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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p16sheLL

Plasmid

#37320

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-n1-APP

Plasmid

#69924

Purpose

mammalian expression of human APP fused to EGFP

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

APP (APP Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Plasmid

#60903

Purpose

Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Cas9 dead

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (Neo)

Plasmid

#89180

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-N1

Plasmid

#54567

Purpose

Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mApple

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

Plasmid

#61628

Purpose

Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

Depositor

Dolf Weijers

Article

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

ntdTomato
n2xEGFP

Expression

Plant

Mutation

2 copies of EGFP with N-terminal SV40 NLS

Promoter

Auxin-dependent DR5 promoter containing 9 reverse…

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM1-FIRE-pHLy

Plasmid

#170775

Purpose

Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

Depositor

Aimee Kao

Article

Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

Insert

LAMP1 (LAMP1 Human)

Use

Lentiviral

Tags

mCherry and mTFP1

Expression

Mammalian

Mutation

84 nucleotide Signal Sequence switched to the beg…

Promoter

CMV

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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