2,225 results

1. pcDNA3.1 myc-PITPbeta

   Plasmid

   #58278

   Purpose

   mammalian expression of rat PITP beta

   Depositor

   Shamshad Cockcroft

   Insert

   PITP beta (Pitpnb Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Myo 1b-myc

   Plasmid

   #135064

   Purpose

   Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

   Depositor

   Martin Bähler

   Article

   Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

   Insert

   Myo 1b with 5' UTR (Myo1b Rat)
   

   Use

   Tags

   Linker and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-syn-SnFR-gamma2-minWPRE

   Plasmid

   #165497

   Purpose

   AAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisation

   Depositor

   Andrew Plested

   Article

   Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

   Insert

   iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VAMP2-12aa-mCit

   Plasmid

   #53237

   Purpose

   Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   12aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pTrcHis-p47

   Plasmid

   #21268

   Depositor

   Hemmo Meyer

   Article

   Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

   Insert

   p47 (Nsfl1c Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-C1-PRKAA1ΔC

   Plasmid

   #30306

   Depositor

   Jay Brenman

   Article

   Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

   Insert

   AMP-activated Protein Kinase α1 ΔC (Prkaa1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids Pro 526 to Gln 548

   Promoter

   Available since

   Aug. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

   Plasmid

   #51473

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

   Depositor

   Tobias Bonhoeffer, Tobias Rose, Mark Huebener

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Insert

   mRuby2-P2A-GCaMP6m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mTagRFP-T-Lysosomes-20

   Plasmid

   #58022

   Purpose

   Localization: Lysosome Membrane, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   LAMP1
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pEF SYT1-HaloTag

   Plasmid

   #197281

   Purpose

   Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 and HaloTag (Syt1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EF1alpha

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBK-YE2-BE3

    Plasmid

    #85176

    Purpose

    Expresses YE2-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-mEGFP(A206K)-paCaMKII(T286A)

    Plasmid

    #165439

    Purpose

    Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(T286A)
    

    Use

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    T286A: Autophosphorylation deficient mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MUNC18-2

    Plasmid

    #186687

    Purpose

    Expresses MUNC18-2 in insect cells

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    MUNC18-2 (Stxbp2 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-SypHluorin 1x (S1x)

    Plasmid

    #37003

    Depositor

    Yongling Zhu, Stephen Heinemann

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin (Syp Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    One copy of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZac2.1-GfaABC1D-lck-jGCaMP8m

    Plasmid

    #176760

    Purpose

    AAV-mediated expression of membrane bound GCaMP8m under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Has Service

    AAV1 and AAV9

    Insert

    lck-jGCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KIF5C(1-560)-2xmCitrine-GFP11

    Plasmid

    #61667

    Purpose

    Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mRuby-Lysosomes-20

    Plasmid

    #55872

    Purpose

    Localization: Lysosome Membrane, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. Kv2.1/RBG4

    Plasmid

    #196947

    Purpose

    Plasmid expressing an antigen targeting Kv2.1 (RBG4)

    Depositor

    James Trimmer

    Insert

    Kv2.1 (Kcnb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mTagBFP-Cx43-7

    Plasmid

    #55256

    Purpose

    Localization: Gap Junctions, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    mTagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

    Plasmid

    #179074

    Purpose

    Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TEF2p-Scarlet(rfp)-Link-2xPH
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO-sh-Nix

    Plasmid

    #100770

    Purpose

    Lentiviral expression of shRNA targeting Nix

    Depositor

    Joseph Gordon

    Article

    da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

    Insert

    Lenti-sh-Nix
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-dCpf1-BE-YEE

    Plasmid

    #107687

    Purpose

    Expresses dLbCpf1-BE-YEE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YEE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCIS-krPAM1

    Plasmid

    #25456

    Depositor

    Richard Mains

    Article

    Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

    Insert

    PAM1 (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

23. PKC zeta DN

    Plasmid

    #21249

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K281M

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pMK285 (Stag-3FLAG-Bsr)

    Plasmid

    #72801

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Bsr
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ratGLUT2

    Plasmid

    #14946

    Depositor

    Bernard Thorens

    Article

    Thorens et al J Biol Chem. 1996 Apr 5. 271(14):8075-81

    Insert

    GLUT2 (Slc2a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pET30-Npl4

    Plasmid

    #21267

    Depositor

    Hemmo Meyer

    Article

    Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

    Insert

    Npl4 (Nploc4 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pSLIK S6K (E389-deltaCT) neo

    Plasmid

    #58516

    Purpose

    Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pCAG-IRES2-p75NTR-GFP

    Plasmid

    #182947

    Purpose

    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal

    Depositor

    Pantelis Tsoulfas

    Insert

    p75NTR-mGFP
    

    Use

    Tags

    HA tag and mGFP

    Expression

    Mammalian

    Mutation

    The leader sequence is from preprotrypin

    Promoter

    CAG

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid

    #167573

    Purpose

    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Ribo-GCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mEmerald-PSD95-N-14

    Plasmid

    #54239

    Purpose

    Localization: Membrane Scaffold, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    PSD95
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge