We narrowed to 29,791 results for: REP

1. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

   Plasmid

   #192401

   Purpose

   Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-Rluc-B3RT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

   Plasmid

   #192400

   Purpose

   On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-Rluc-B3RT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

   Plasmid

   #192402

   Purpose

   On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

   Plasmid

   #192405

   Purpose

   Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   B3RT-Act2-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

   Plasmid

   #192403

   Purpose

   Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2-foxd4l1.1-AB4

   Plasmid

   #185513

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   IDILGE replaced with AAAAAA

   Promoter

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-foxd4l1.1-AB2

   Plasmid

   #185512

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   IDIL replaced with AAAAAA

   Promoter

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2-foxd4l1.1-QR-myc

   Plasmid

   #185521

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   myc

   Expression

   Mutation

   replaces Q>R upstream of GARQYLNI

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2-foxd4l1.1-QR

   Plasmid

   #185520

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   replaces Q>R upstream of GARQYLNI

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-foxd4l1.1-LA-myc

    Plasmid

    #185519

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    myc

    Expression

    Mutation

    replaces L>A upstream of GARQYLN

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2-foxd4l1.1-LA

    Plasmid

    #185518

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    replaces L>A upstream of GARQYLNI

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2-foxd4l1.1-GARP-myc

    Plasmid

    #185517

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    myc

    Expression

    Mutation

    Replaces Q to P in GARQYNLI

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2-foxd4l1.1-GARP

    Plasmid

    #185516

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Replaces Q to P in GARQYNLI

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2-foxd4l1.1-GARG-myc

    Plasmid

    #185515

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    myc

    Expression

    Mutation

    Replaces Q to G in GARQYNLI

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2-foxd4l1.1-GARG

    Plasmid

    #185514

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Replaces Q to G in GARQYNLI

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCm N-term MBP SMT3 NikJC199U

    Plasmid

    #174363

    Purpose

    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, MBP, SMT3, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFB_ROSA26_SpCas9_gRNAs

    Plasmid

    #174703

    Purpose

    Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

    Depositor

    Beverly L. Davidson

    Article

    Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

    Insert

    SpCas9 dual gRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCTCON2-BXG-altTAG

    Plasmid

    #158132

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCTCON2-BXG-altTAG2

    Plasmid

    #158134

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the second serine residu…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCTCON2-BXG-altTAG3

    Plasmid

    #158136

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the third alanine residu…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCTCON2-BXG-altTAG4

    Plasmid

    #158137

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the third serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Antares-SEPLuc IRESv24

    Plasmid

    #141087

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEPLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-PTEN-3'UTR

    Plasmid

    #97204

    Purpose

    Expresses PTEN 3'UTR in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Kerwin et al Elife. 2020 Apr 21;9. pii: 51019. doi: 1

    Insert

    PTEN 3' UTR (PTEN Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. K8.1Pr-pGL4.16-OriLyt

    Plasmid

    #131038

    Purpose

    Firefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replication

    Depositor

    Britt Glaunsinger

    Article

    Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

    Insert

    KSHV Lytic Origin of replication
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC 3GLA

    Plasmid

    #71762

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LLhE_AW-pHG165

    Plasmid

    #90062

    Purpose

    Constitutive expression of a chimeric LacI:CelR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhE_AW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I48V/Q55A/Q60R

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LLhS-pHG165a

    Plasmid

    #90040

    Purpose

    Constitutive expression of a chimeric LacI:GalS transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LLhF-pHG165a

    Plasmid

    #90041

    Purpose

    Constitutive expression of a chimeric LacI:FruR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LLhT-pHG165a

    Plasmid

    #90042

    Purpose

    Constitutive expression of a chimeric LacI:TreR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LLhR-pHG165a

    Plasmid

    #90043

    Purpose

    Constitutive expression of a chimeric LacI:RbsR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge