We narrowed to 28,446 results for: REP

1. pLenti myc-NL-GW

   Plasmid

   #113454

   Purpose

   Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   cmyc-NanoLuc

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LSB-hsa-miR-7-5p

   Plasmid

   #103723

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-7-5p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LSB-hsa-miR-101-3p

   Plasmid

   #103165

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-101-3p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 GW-mCit-PA

   Plasmid

   #113449

   Purpose

   ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Gateway shuttle vector

   Tags

   mCitrine-ProteinA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-TetO-Tfap2c

   Plasmid

   #128826

   Purpose

   dox-dependent expression of mouse Tfap2c

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

   Insert

   Tfap2c (Tfap2c Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiPGK Puro DEST JNKKTR AE Clover

   Plasmid

   #90240

   Purpose

   Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (AE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LSB-hsa-miR-516b-5p

   Plasmid

   #103601

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-516b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-516b-5p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 PA-mCit-GW

   Plasmid

   #113448

   Purpose

   ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Gateway shuttle vector

   Tags

   ProteinA-mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCYC1pr-GNSI (LEU)

   Plasmid

   #111450

   Purpose

   GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

   Depositor

   Alexey Merz

   Article

   Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

   Inserts

   CYC1 Promoter (CYC1 Budding Yeast)
   Nyv1 Cytosolic Domain (aa 6-230)
   Snc1 Transmembrane Domain (TMD)
   SUC2 CDS
   SUC2 terminator
   

   Use

   Tags

   mGFP5

   Expression

   Yeast

   Mutation

   M109I (Naturally occurring SNP) and The first 21 …

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-hs-3xHA-delta6xNHL-LIN-41

    Plasmid

    #52722

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 593–868

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102891

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXs-ms-c-Myc-V394D

    Plasmid

    #50775

    Purpose

    retroviral expression of mouse c-Myc V394D mutant

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc V394D (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V394D (does not bind to Miz-1)

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LSB-hsa-miR-138-5p

    Plasmid

    #103233

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-138-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-138-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKrox24(MapErk)DsRed

    Plasmid

    #200114

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence, no other pr…

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5FRTTO-NHASt-codoptUS2

    Plasmid

    #135102

    Purpose

    Codon-optimized HSV-1 US2 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 US2 codon optimized
    

    Use

    Tags

    HA-Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSB-hsa-miR-19b-3p

    Plasmid

    #103327

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-19b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-19b-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-hu-c-Myc-dN2

    Plasmid

    #50774

    Purpose

    retroviral expression of human c-Myc dN2 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc -dN2 (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 42-439

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMXs-hu-c-Myc-dN1

    Plasmid

    #50773

    Purpose

    retroviral expression of human c-Myc dN1 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc -dN1 (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 14-439

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti GW-mCit-PA

    Plasmid

    #113457

    Purpose

    Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCitrine-ProteinA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti GW-NL-myc

    Plasmid

    #113455

    Purpose

    Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral and Luciferase

    Tags

    NanoLuc-cmyc

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCS2 Notch1 LNG-6MT

    Plasmid

    #41738

    Depositor

    Jeffrey Nye, Raphael Kopan

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

25. LSB-hsa-let-7f-5p

    Plasmid

    #103156

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7f-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LSB-hsa-miR-133a-3p

    Plasmid

    #103221

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-133a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-133a-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5FRTTO-NHASt-codoptUL50

    Plasmid

    #135101

    Purpose

    Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL50 codon optimized
    

    Use

    Tags

    HA-Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 PA-NL

    Plasmid

    #113445

    Purpose

    ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LSB-hsa-miR-125b-5p

    Plasmid

    #103191

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-125b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-125b-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 PA-mCit-NL

    Plasmid

    #113444

    Purpose

    ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge