Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,677 results for: tyr

Clear keywords
Showing: 1201 - 1240 of 1677 results

  1. pGFP FRNK

    Plasmid
    #50518
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Article
    Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.
    Insert
    protein tyrosine kinase, mutated
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    truncated (NT2186-3268)
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pMAH-POLY

    Plasmid
    #64915
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-
    Inserts
    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    Tags
    None
    Expression
    Mammalian
    Promoter
    CMV and U6/H1
    Available Since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64915-plasmid-map-sequence-id-142448

    Enlarge

  6. Membrane EGFP

    Plasmid
    #198056
    Purpose
    Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Insert
    LCK membrane EGFP (LCK A. victoria, Human, Synthetic)
    Use
    Sea urchin, mammal, zebrafish
    Tags
    EGFP
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198056-plasmid-map-sequence-id-389155

    Enlarge

  8. pRF0G-IodoY

    Plasmid
    #113645
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    IodoYRS and tRNA gene
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113645-plasmid-map-sequence-id-217127

    Enlarge

  9. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

    Plasmid
    #201186
    Purpose
    Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    PTPN2, transcript variant 1 (PTPN2 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201186-plasmid-map-sequence-id-398097

    Enlarge

  10. pGFP FAT

    Plasmid
    #50517
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Article
    Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.
    Insert
    protein tyrosine kinase, domain
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    truncated (NT2630-3268)
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

    Plasmid
    #201187
    Purpose
    Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    PTPN2, transcript variant 2 (PTPN2 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201187-plasmid-map-sequence-id-398106

    Enlarge

  20. pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald

    Plasmid
    #201188
    Purpose
    Lentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    PTPN2, transcript variant 3 (PTPN2 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201188-plasmid-map-sequence-id-398098

    Enlarge

  21. pLT237

    Plasmid
    #174300
    Purpose
    Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.
    Depositor
    Lee Lynd
    Article
    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1
    Insert
    PolC homology arm
    Expression
    Bacterial
    Mutation
    changed Cysteine 669 to Tyrosine
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    174300-plasmid-map-sequence-id-392933

    Enlarge

  23. pBN008

    Plasmid
    #167949
    Purpose
    express E. coli E69 (O9a:K30) Wzc with K540M mutation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167949-plasmid-map-sequence-id-344798

    Enlarge

  25. pTH859-gst-optec Y172M

    Plasmid
    #89593
    Purpose
    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed tyrosine 172 to methionine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89593-plasmid-map-sequence-id-206040

    Enlarge

  28. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

    Enlarge

  29. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

    Enlarge

  30. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60621-plasmid-map-sequence-id-208224

    Enlarge

  31. pQCXIP-cathepsinD-YMH

    Plasmid
    #182644
    Purpose
    Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    cathepsinD
    Use
    Retroviral
    Tags
    His6-tag, TS site from proCCK, and myc-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182644-plasmid-map-sequence-id-358628

    Enlarge

  34. pBA-mCherry-EGFP-PIM

    Plasmid
    #111758
    Purpose
    Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Article
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    chicken beta-actin
    Available Since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111758-plasmid-map-sequence-id-222504

    Enlarge

Showing: 1201 - 1240 of 1677 results