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Showing: 1201 - 1240 of 1608 results

  3. pCT

    Plasmid
    #60620
    Purpose
    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60620-plasmid-map-sequence-id-99574

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  9. pET-30a(+)-Syk-tSH2

    Plasmid
    #111269
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111269-plasmid-map-sequence-id-215599

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  15. pET28-NFHΔ36-72

    Plasmid
    #232060
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 36-72
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232060-plasmid-map-sequence-id-464069

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  16. pET28-NFHΔ462-638

    Plasmid
    #232061
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 462-638
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232061-plasmid-map-sequence-id-464068

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  17. pET28-NFMtail

    Plasmid
    #232056
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232056-plasmid-map-sequence-id-464064

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  18. pET28-NFHtail

    Plasmid
    #232057
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232057-plasmid-map-sequence-id-464061

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  19. pAMC042z21

    Plasmid
    #209747
    Purpose
    RepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL bla
    Depositor
    Ahmed Badran
    Article
    Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103
    Insert
    sfGFP - tRNA operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…
    Promoter
    Available since
    Oct. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209747-plasmid-map-sequence-id-444960

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  28. pRH3243

    Plasmid
    #210187
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    R33P
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210187-plasmid-map-sequence-id-416327

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  29. pRH3176

    Plasmid
    #210178
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    G141S
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210178-plasmid-map-sequence-id-416316

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  30. pRH3145

    Plasmid
    #210169
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    D147V
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210169-plasmid-map-sequence-id-416315

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  31. pRH3139

    Plasmid
    #210167
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    R33G
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210167-plasmid-map-sequence-id-416313

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  32. pRH3142

    Plasmid
    #210168
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    D147G
    Promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210168-plasmid-map-sequence-id-415820

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  33. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid
    #190174
    Purpose
    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway
    Depositor
    Gary Rudnick
    Article
    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.
    Insert
    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    Use
    Tags
    FLAG, His6, and c-myc
    Expression
    Mammalian
    Mutation
    changed Cys109 to alanine, changed tyr107 to cyst…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190174-plasmid-map-sequence-id-377202

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Showing: 1201 - 1240 of 1608 results