We narrowed to 29,292 results for: REP

1. Strep-HA-VCF1 N167A

   Plasmid

   #223021

   Purpose

   Expression of Strep-HA-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.

   Depositor

   Niels Mailand

   Article

   Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

   Insert

   VCF1 (VCF1 Human)
   

   Use

   Tags

   Strep-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL1-FETCH2-StrepII

   Plasmid

   #210649

   Purpose

   In vitro expression of FETCH reporter from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

   Insert

   FETCH2
   

   Use

   Synthetic Biology

   Tags

   Strep-TagII

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPglL-strep-mCherry

   Plasmid

   #196664

   Purpose

   Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   PglL
   

   Use

   Tags

   TEV-cleavable distrep tag II and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPglL-strep-mClover3

   Plasmid

   #196665

   Purpose

   Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   PglL
   

   Use

   Tags

   TEV-cleavable di-strep tag II and mClover3

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWzyE-GST-distrepII

   Plasmid

   #196666

   Purpose

   Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   GST and TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWzyE-strep-mClover3

   Plasmid

   #196668

   Purpose

   Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   TEV-cleavable di-strep tag II and mClover3

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS-BCD-Strep-PC-σ

   Plasmid

   #214037

   Purpose

   Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PC-σ
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-RepoMan-RATA

   Plasmid

   #184270

   Purpose

   Expresses shRNA resistant GFP-RepoMan RATA Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Use

   Tags

   3xHA and GFP

   Expression

   Mammalian

   Mutation

   V383A, F395A

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-RepoMan-T394A

   Plasmid

   #204994

   Purpose

   Expresses shRNA resistant GFP-RepoMan T394A Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell devision cycle associated 2 (CDCA2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Threonine 394 to Alanin

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-RepoMan-S893D

    Plasmid

    #204995

    Purpose

    Expresses shRNA resistant GFP-RepoMan S893D Mutant

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    cell division cycle associated 2 (CDCA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 893 to Aspartic acid

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-RPL3L-TwStrep

    Plasmid

    #208649

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949

    Depositor

    Quanfu Ma

    Insert

    human RPL3L (ENST00000268661) (RPL3L Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    contain rs753338989, rs746044959, rs752134949, pr…

    Promoter

    T7

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-C17orf53-Strep

    Plasmid

    #211446

    Purpose

    generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-Tag

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Use

    Tags

    Strep-Tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-eIF4E-TwStrep

    Plasmid

    #208638

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

    Depositor

    Quanfu Ma

    Insert

    human eIF4E (ENST00000450253) (EIF4E Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-RPL3-Twstrep

    Plasmid

    #208623

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) (RPL3 Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SARS-CoV-2 N-2Strep

    Plasmid

    #210376

    Purpose

    Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-COV-2 N (N SARS-CoV-2)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Strep-TGT-His

    Plasmid

    #206495

    Purpose

    Expresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His Tag

    Depositor

    Neal Devaraj

    Article

    Tota et al J Am Chem Soc. 2023 Apr 12;145(14):8099-

    Insert

    E. coli tRNA Guanine Transglycosylase
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Annexin A7-TEV-TwinStrep

    Plasmid

    #198635

    Purpose

    Annexin A7 fibrillization and lipid binding

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 (ANXA7 Human)
    

    Use

    Tags

    TEV-GS-Dual Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFsynW SYT1deltaC2AB reporter

    Plasmid

    #197279

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HyWnt3-ΔRep-D3:Luc

    Plasmid

    #192990

    Purpose

    Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)

    Depositor

    Brigitte Galliot

    Article

    Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

    Insert

    Hydra Wnt3 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial deletion of the Wnt3 repressor element-D3

    Promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HyWnt3-ΔRep-D1:Luc

    Plasmid

    #192988

    Purpose

    Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)

    Depositor

    Brigitte Galliot

    Article

    Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

    Insert

    Hydra Wnt3 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial deletion of the Wnt3 repressor element-D1

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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