20,129 results

Showing: 9451 - 9480 of 20129 results

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pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

Plasmid

#167583

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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PB-SAM-Dest

Plasmid

#102563

Purpose

Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

N/A

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChR2(H134R)-GFP

Plasmid

#58880

Purpose

AAV expression of ChR2(H134R)-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Has Service

AAV Retrograde and AAV8

Insert

ChR2(H134R)-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

H134R

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Hygro EYFP H148Q/I152L

Plasmid

#25874

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q/I152L

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Available Since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

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Ack1-pmCherryC1

Plasmid

#27684

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ack1 (Tnk2 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3 P-cad

Plasmid

#47502

Purpose

Expresses P-cadherin in mammalian cells

Depositor

Alice Wong

Article

Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

Insert

cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk2

Plasmid

#81011

Purpose

Expression of Zdk2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk2

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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PB CAG-sfGFP

Plasmid

#133568

Purpose

CAG sfGFP piggyBac transposon

Depositor

Mario Capecchi

Insert

sfGFP

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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CD3 delta YFP

Plasmid

#11951

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

CD3delta-YFP

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag LCK

Plasmid

#20511

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

LCK (LCK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pMVA2RBS035

Plasmid

#121051

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA Myc DBC1

Plasmid

#35096

Depositor

Osamu Hiraike

Article

Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

Insert

deleted in breast cancer 1 (CCAR2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pHMM

Plasmid

#67717

Purpose

Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Hexahistidine/Maltose binding protein/MS2 coat protein fusion

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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CMV566 empty vector

Plasmid

#33349

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only
pHR iRFP-ZtSH2

Plasmid

#188627

Purpose

Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

ZtSH2

Use

Lentiviral

Tags

iRFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-TRV1

Plasmid

#180515

Purpose

Agrobacterium tumefaciens-based expression of TRV1

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA1

Expression

Plant

Promoter

35S CaMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-ReaChR-citrine

Plasmid

#50954

Purpose

ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

truncated human Synapsin promoter

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTEC27

Plasmid

#30182

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

tdTomato

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA

Plasmid

#12522

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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ALKBH5-dCas9

Plasmid

#134783

Purpose

Expression fusion protein ALKBH5-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Tag5Amyc-GSK3b WT

Plasmid

#16260

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

GSK3 beta (GSK3B Human)

Tags

Myc

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pBABE puro K-Ras V12

Plasmid

#9052

Purpose

Retroviral expression of a constitutively active form of K-Ras

Depositor

William Hahn

Insert

KRAS (KRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V. Constitutively active.

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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Cytosolic FAK biosensor

Plasmid

#78300

Purpose

FRET biosensor. Visualization of cytosolic FAK activity in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Cytosolic FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-a1AR-EYFP

Plasmid

#20947

Purpose

Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-a1AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pGWB401

Plasmid

#74795

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-MRLC T18A,S19A

Plasmid

#35681

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18A and S19A

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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20,129 results