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Plasmid#71481PurposeEncodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 3 (SENP3 Human)
UseTagsHexahistidineExpressionBacterialMutationDeleted amino acids 1-306 (catalytic domain only)PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22-6His-SUMO-Nsp10
Plasmid#154960PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21-GFP-AURKA-mCherry
Plasmid#99876PurposeExpression of GFP-AURKA-mCherry for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFP and mCherryExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsHisExpressionBacterialMutationE147Q, E148QPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-APEX2
Plasmid#117751PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-N-IDR
Plasmid#166440PurposeBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion proteinDepositorInsertmEGFP, YAP-N-IDR(1-164) (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[D134TAG+N150TAA]
Plasmid#164582Purposesuperfolder GFP with D134TAG and N150TAA mutationsDepositorInsertsfGFP-134TAG+150TAA
UseTags6xHisExpressionBacterialMutationD134TAG + N150TAA MutationsPromoterT7Available sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT N-His Ana GvpC in pET28a
Plasmid#153294PurposeExpresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (N-terminal his-tagged)
UseTagsHis-tagExpressionBacterialMutationAdded a hexahistidine tag after the methionine at…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp7 C186A-His
Plasmid#11826DepositorInsertCaspase 7 catalytic mutant (CASP7 Human)
UseTags6xHisExpressionBacterialMutationC186APromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pF714-pET28a-Hs-FL-METTL20-NHis wt
Plasmid#85115Purposeexpression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tagDepositorInserthuman full-length METTL20 (METTL20 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT5
Plasmid#53560Purposebacterial expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Gluc-EL222-his
Plasmid#172097PurposeExpresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteriaDepositorInsertGaussia Luciferase - EL222
UseTagsExpressionBacterialMutationGluc mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6-H133Y
Plasmid#20279DepositorInsertSirt6 (Sirt6 Mouse)
UseTagsHisx6ExpressionBacterialMutationHistidine 133 to TyrosinePromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C93A
Plasmid#133910PurposeBacterial expression of His6-Ubc9-C93ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 93 to AlaninePromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-Strep-TEV-Nsp14
Plasmid#154955PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertStrep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
UseTagsStrep-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a(+) LdtMt2 (131-408)
Plasmid#59337PurposeExpresses LdtMt2 for L,D-transpeptidationDepositorInsertLdtMt2 (131-408)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(1-611)
Plasmid#39336DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop A
Plasmid#171776PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-SpyTag003 Loop A
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues V…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
UseTags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C138A
Plasmid#133911PurposeBacterial expression of His6-Ubc9-C138ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 138 to AlaninePromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-myc-His
Plasmid#67864Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tagDepositorInsertMRPP3 (KIAA0391 Human)
UseTagsHis and mycExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21 Catalytic Domain of MALT1
Plasmid#48970PurposeExpresses catalytic domain of MALT1 in e.coli AA 329-566DepositorInsertMALT1 (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains AA 329-566PromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TAF4(827-909)
Plasmid#105620Purposebacterially express TAF4 HFDDepositorInsertTAF4-HFD(827-909) (Taf4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mCherry-TEAD4-IDR
Plasmid#166442PurposeBacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion proteinDepositorInsertmCherry, TEAD4-IDR (TEAD4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P12A P13A)
Plasmid#62066PurposeExpression of human p53 (residues 1-73) (P12A, P13A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13APromoterT7Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-deltaPYD-GST
Plasmid#51534PurposeExpress GST-tagged HIN domain of mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsGST and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/G51PEKN
Plasmid#118583PurposeBacterial expression of E.coli DHFR N23PP and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, G51 mutated to P51, a Pro res…PromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/S148A
Plasmid#110829PurposeBacterial expression of E.coli DHFR N23PP/S148A mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-Nsp14-TEV-Strep
Plasmid#154953PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertNsp14-TEV-Strep (ORF1ab SARS-CoV-2)
UseTagsTEV-StrepExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22-Strep-TEV-Nsp10
Plasmid#154958PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertStrep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
UseTagsStrep-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-Hisx6-Sirt6-S56A
Plasmid#20278DepositorInsertSirt6 (Sirt6 Mouse)
UseTagsHisx6ExpressionBacterialMutationSerine 56 to AlaninePromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-PYD-GST
Plasmid#51531PurposeExpress GST-tagged PYD of hAIM2 in bacteriaDepositorInsertAIM2 (AIM2 Human)
UseTagsGST and T7ExpressionBacterialMutationdeletion of amino acids 102-343Promoterlac operatorAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EC12-129/138
Plasmid#47926PurposeExpresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.DepositorInsertE-Cadherin EC12
UseTags6xHis and TEV CleavageExpressionBacterialMutationMutations C9A, K129C, D138CPromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-TEV-SpyCatcher3
Plasmid#235149PurposeBacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertSpyCatcher3
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH81 pET21b Rat1-His
Plasmid#231650PurposeBacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tagDepositorInsertRat1 (RAT1 Budding Yeast)
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29_anti-SARS-CoV-2-NM1226
Plasmid#209422PurposeExpress anti-SARS-CoV-2-Nanobody NM1226 in E. coliDepositorInsertanti-SARS-CoV-2-NM1226
UseTagsMyc-6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-SARS-CoV-2-NM1230
Plasmid#209423PurposeExpress anti-SARS-CoV-2-Nanobody NM1230 in E. coliDepositorInsertanti-SARS-CoV-2-NM1230
UseTagsMyc-6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-MERS-Nanobody-VHH-55
Plasmid#209417PurposeExpress anti-MERS-Nanobody-VHH-55 in E. coliDepositorInsertanti-mers-nanobody-vhh-55
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-MERS-Nanobody-VHH-83
Plasmid#209418Purposeexpress anti-MERS-Nanobody-VHH-83 in E. coliDepositorInsertanti-mers-nanobody-vhh-83
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-MERS-Nanobody-VHH-84
Plasmid#209419PurposeExpress anti-MERS-Nanobody-VHH-84 in E. coliDepositorInsertanti-mers-nanobody-vhh-83
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-SARS-CoV-2-Nanobody-C5
Plasmid#209420PurposeExpress anti-SARS-CoV-2-Nanobody C5 in E. coliDepositorInsertanti-SARS-CoV-2-Nanobody-C5
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-SARS-CoV-2-Nanobody-F2
Plasmid#209421PurposeExpress anti-SARS-CoV-2-Nanobody F2 in E. coliDepositorInsertanti-SARS-CoV-2-Nanobody-F2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-MERS-Nanobody-VHH-1
Plasmid#206890PurposeExpress anti-MERS-Nanobody-VHH-1 in E. coliDepositorInsertanti-mers-nanobody-vhh-1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21-pelB-LaG94-1--LaG94-14
Plasmid#228973PurposeFor bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaG94-1--LaG94-14
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiC
Plasmid#229222PurposeFor expression of T. elongatus KaiC proteinDepositorInsertkaiC (kaiC T. elongatus)
UseTagsSUMO-FLAGExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ULP1-405-621
Plasmid#229224PurposeFor expression of ULP1 proteinDepositorInsertULP1 (ULP1 Budding Yeast)
UseTagsExpressionBacterialMutation405-621 (truncated)PromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-SeKaiB-FLAG
Plasmid#229219PurposeFor expression of S. elongatus KaiB proteinDepositorInsertkaiB (kaiB S. elongatus)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-Bcx
Plasmid#191195PurposeFusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Bcx fusion
UseTags6xHis and Bacillus circulans xylanaseExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only