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178,737 results

Showing: 9841 - 9870 of 178737 results

  3. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #69138
    Purpose
    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo
    Depositor
    Takuji Iwasato
    Publication
    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    Tags
    none
    Expression
    Mammalian
    Available since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69138-plasmid-map-sequence-id-165113

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  4. pNTI194

    Plasmid
    #84266
    Purpose
    Expresses Avi-tagged RPL1 and humanized birA
    Depositor
    Nicholas Ingolia
    Publication
    Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    RPL1 (RPL10A (*see below), Human)
    humanized birA
    Tags
    Avi tag, Flag, Gly/Ser linker, and TEV site
    Expression
    Mammalian
    Mutation
    R33A, A34E (*see below)
    Promoter
    CMV and P2A peptide
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84266-plasmid-map-sequence-id-160813

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  6. p5E--3mnx1

    Plasmid
    #74632
    Purpose
    Can be used to drive expression specifically in zebrafish motor neurons
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    -3mnx1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74632-plasmid-map-sequence-id-207910

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  12. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Publication
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80085-plasmid-map-sequence-id-197645

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  13. pMito-miRFP703

    Plasmid
    #80000
    Purpose
    Mitochondria labeling with near-infrared fluorescent protein miRFP703
    Depositor
    Vladislav Verkhusha
    Publication
    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cytochrome C oxidase subunit VIII (COX8A Human)
    Tags
    miRFP703
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80000-plasmid-map-sequence-id-150089

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  14. pGCaMP6m-N3-TPC2

    Plasmid
    #80147
    Purpose
    GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real time
    Depositor
    Santiago Di Pietro
    Publication
    Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPCN2 (TPCN2 Human)
    Tags
    GCaMP6m
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80147-plasmid-map-sequence-id-150677

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  16. pCIP

    Plasmid
    #79009
    Purpose
    For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.
    Depositor
    Reuben Shaw
    Publication
    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    pCAGGs
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79009-plasmid-map-sequence-id-151714

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  17. Synapsin sHRPa-NRX in FSW lentiviral vector

    Plasmid
    #73147
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-NRX
    Use
    Lentiviral
    Tags
    2x HA tag and Flag tag
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73147-plasmid-map-sequence-id-134524

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  18. CAG sHRPb-NLG

    Plasmid
    #73144
    Purpose
    Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPb-NLG (Nlgn1 Mouse, Synthetic)
    Tags
    V5 tag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73144-plasmid-map-sequence-id-134514

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  22. pRubiC-T2A-Cas9

    Plasmid
    #75347
    Purpose
    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    mCherry (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    75347-plasmid-map-sequence-id-232926

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  24. PXL

    Plasmid
    #75349
    Purpose
    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75349-plasmid-map-sequence-id-266810

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Showing: 9841 - 9870 of 178737 results