We narrowed to 6,962 results for: tet on

1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-6

   Plasmid

   #129058

   Purpose

   targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting mouse TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA6 (mouseTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-7

   Plasmid

   #129059

   Purpose

   targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting mouse TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA7 (mouseTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-8

   Plasmid

   #129060

   Purpose

   targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting mouse TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA8 (mouseTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

   Plasmid

   #129047

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA7 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

   Plasmid

   #129049

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA9 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

   Plasmid

   #129050

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA10 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

   Plasmid

   #129051

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA11 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

   Plasmid

   #129052

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA12 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

   Plasmid

   #129040

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA12 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid

    #129042

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA2 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid

    #129043

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA3 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid

    #129044

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA4 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

    Plasmid

    #129045

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA5 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid

    #129046

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA6 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

    Plasmid

    #129033

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA5 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid

    #129034

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA6 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid

    #129035

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA7 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid

    #129037

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA9 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid

    #129038

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA10 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid

    #129039

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA11 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid

    #129030

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA2 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid

    #129031

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA3 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid

    #129032

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA4 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid

    #121803

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + EF1a::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121807

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Gal4-TET1-878-911-I894A(pL84)

    Plasmid

    #124404

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(I894A mutation) (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    I894A

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Gal4-TET1-878-911-T898A(pL84)

    Plasmid

    #124403

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(T898A mutation) (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    T898A

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Gal4-TET1-878-911-L900A(pL84)

    Plasmid

    #124402

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(L900A mutation) (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    L900A

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Gal4-TET1-878-911-L897A(pL84)

    Plasmid

    #124401

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(L897A mutation) (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    L897A

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

    Plasmid

    #118593

    Purpose

    Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha/TRE3G

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid

    #227246

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α and pTRE3G

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

    Plasmid

    #210020

    Purpose

    tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-mini-CMV

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

    Plasmid

    #199349

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOEu

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid

    #227245

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α and pTRE3G

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

    Plasmid

    #199350

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

    Plasmid

    #12153

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

    Plasmid

    #166611

    Purpose

    Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag

    Plasmid

    #231594

    Purpose

    Express high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Kozak sequence-Tornado-10xSunTag-2xALFATag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

    Plasmid

    #78101

    Purpose

    Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

    Plasmid

    #199359

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

    Plasmid

    #193349

    Purpose

    tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

    Plasmid

    #176630

    Purpose

    Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

    Plasmid

    #199348

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-TEV

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

    Plasmid

    #101349

    Purpose

    2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta tat delta TAR delta env GFP in Nef Tet ope…

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

    Plasmid

    #123657

    Purpose

    DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

    Depositor

    Stanley Qi

    Article

    Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

    Insert

    mCherry-P2A-DD-AcrIIA4
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    mCherry-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mU6

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

    Plasmid

    #78349

    Purpose

    Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

    Plasmid

    #186740

    Purpose

    Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    RAB10, RABIF (RAB10, RABIF Human)
    

    Use

    Lentiviral

    Tags

    RAB10-T2A-RABIF-P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP

    Plasmid

    #199358

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2

    Plasmid

    #210019

    Purpose

    tetO-OKS (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + Sox2)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2 (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-mini-CMV

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge