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pCW57-MCS1-P2A-MCS2 (Hygro)

Plasmid

#80922

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

Plasmid

#84469

Purpose

pAAV-RAM-EGFP

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

EGFP

Use

AAV

Promoter

TRE

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.GfaABC1D.PI.Cre.SV40

Plasmid

#105603

Purpose

AAV expression of Cre from GfaABC1D promoter

Depositor

Baljit Khakh

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

GfaABC1D

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-6HA

Plasmid

#99520

Purpose

C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

6xHA

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTA7001-DEST

Plasmid

#71745

Purpose

GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Expression

Plant

Promoter

GAL4

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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LBR pEGFP-N2 (646)

Plasmid

#61996

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LBR (LBR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pIRESneo-EGFP-alpha Tubulin

Plasmid

#12298

Depositor

Patricia Wadsworth

Article

Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

Insert

EGFP-alpha Tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Puro (658-5)

Plasmid

#17448

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHluorin

Plasmid

#70113

Purpose

Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHluorin (Cd63 Mouse, Aequorea victoria)

Tags

HA-Tag and pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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EYFP-hCaM

Plasmid

#47603

Depositor

Emanuel Strehler

Article

Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

Insert

CaM (CALM3 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pKD3

Plasmid

#45604

Depositor

Barry L. Wanner

Article

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-cat-FRT

Expression

Bacterial

Promoter

R6K

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-3XMyc-EGFP-OMP25

Plasmid

#83355

Purpose

For tagging mitochondria with Myc epitopes (Control-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XMyc-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3h

Plasmid

#208699

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3h

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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FUdeltaGW-rtTA

Plasmid

#19780

Purpose

3rd generation lentiviral vector

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

reverse tetracycline transactivator

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6b

Plasmid

#207852

Purpose

Mammalian expression of PE6b prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6b

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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NG-ABE8e

Plasmid

#138491

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (NG)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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murine TCR OTI-2A.pMIG II

Plasmid

#52111

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

T2A (from Thosea asigna virus)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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