8,717 results

1. PB_tre_dCas9_VP160

   Plasmid

   #126031

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with VP160
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TagBFP2-CAAX

   Plasmid

   #116856

   Purpose

   plasma membrane marker

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   HRAS
   

   Use

   Tags

   TagBFP2

   Expression

   Mammalian

   Mutation

   Amino acids 172-189

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mAG-GAL3

   Plasmid

   #62734

   Purpose

   Mammalian vesicle leakage reporter

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   mAG-Galectin 3
   

   Use

   Lentiviral

   Tags

   mAzami-Green

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-Alpha

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIRV-AP-1-mCherry

   Plasmid

   #118095

   Purpose

   Fluorescent reporter for AP-1 activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with AP-1 responsive element

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1 T2 CRIPR in pX330

   Plasmid

   #72833

   Purpose

   CRISPR/Cas to target the AAVS1 locus in human cells

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   Cas9 and gRNA for targeting the AAVS1 locus in human cells
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-tdTomato

   Plasmid

   #83029

   Purpose

   expresses tdTomato fluorescent protein under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL1142 (pSPIN, pBBR1 backbone)

   Plasmid

   #160730

   Purpose

   Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF.myc.ER-E2-Crimson

   Plasmid

   #38770

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Use

   Tags

   ER retention signal (KDEL), ER signal sequence (M…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. Matrix-roGFP

   Plasmid

   #49437

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   Matrix-roGFP2
   

   Use

   Adenoviral

   Tags

   Cytochrome c oxidase subunit IV mitochondrial tar…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) eGFP

    Plasmid

    #129020

    Purpose

    Expresses eGFP

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-p300

    Plasmid

    #23252

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. DT960

    Plasmid

    #80412

    Purpose

    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC18-mini-Tn7T-Gm-lux

    Plasmid

    #64963

    Purpose

    mini-Tn7 luxCDABE transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    lux operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3 TFAM-mClover

    Plasmid

    #129574

    Purpose

    Expression of mClover fused TFAM

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 TFAM-mScarlet

    Plasmid

    #129573

    Purpose

    Expression of mScarlet fused TFAM

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Olig001

    Plasmid

    #170716

    Purpose

    Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

    Depositor

    Thomas McCown

    Article

    Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

    Insert

    Olig001 capsid gene / rep 2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Luc

    Plasmid

    #45968

    Depositor

    Ralf Kuehn

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. tau/pET29b

    Plasmid

    #16316

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    tau (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-TetO-hNGN2-eGFP-Puro

    Plasmid

    #79823

    Purpose

    Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

    Depositor

    Kristen Brennand

    Article

    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

    Insert

    hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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