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pcDNA3-FLAG-LKB1

Plasmid

#8590

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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tdTomato-pBAD

Plasmid

#54856

Purpose

Localization: Protein Expression Vector , Excitation: 554, Emission: 581

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

6xHis and tdTomato

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227898

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227890

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDARMO_HUWE1_3xFLAG

Plasmid

#187155

Purpose

mammalian cell expression of HUWE1-3xFLAG

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHEE401E

Plasmid

#71287

Purpose

Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Inserts

gRNA scaffold
zCas9

Use

CRISPR; Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Promoter

EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTREtight2

Plasmid

#19407

Depositor

Markus Ralser

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_STRN:1-731

Plasmid

#211230

Purpose

Mammalian expression of full-length human STRN isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

STRN:M1-V731 (STRN Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pML625_RTN4-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227895

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RTN4 homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227910

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML499_SLC3A2-C-term_XTEN80-sfGFP-3xHA_pTMPLT

Plasmid

#227913

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SLC3A2 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304-VHL-IRES-GFP

Plasmid

#187641

Purpose

VHL Expression vector with GFP

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

VHL (VHL Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPdelta

Plasmid

#13394

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPdelta (Cebpd Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-ZO1-C-14

Plasmid

#55165

Purpose

Localization: Tight Junctions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ZO1 (TJP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-FOXP1

Plasmid

#153145

Purpose

Expression of Flag tagged human FOXP1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP1 (FOXP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Inserts

NSP7
NSP8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-KGC-GFP-ER2

Plasmid

#65014

Purpose

AAV-mediated expression of Jaws under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV Retrograde, AAV5, and AAV8

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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