We narrowed to 59,739 results for: Gene
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Plasmid#154885PurposepiggyBac self-reporting transposon with tdTomato marker for mammalian calling cardsDepositorInsertpiggyBac SRT
UseTransposonExpressionMammalianAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF50-2xCSTREP
Plasmid#136211PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50DepositorInsertORF50
ExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF57-2xCSTREP
Plasmid#136217PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57DepositorInsertORF57
ExpressionMammalianPromoterCMVAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTF102
Plasmid#134774PurposeA binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.DepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 MS2 sgRNA
Plasmid#92394PurposeChick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCPDepositorInsertchick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
UseCRISPRExpressionMammalianPromoterchick U6.3Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
DDX39B_pLX307
Plasmid#98331PurposeLentiviral expression of DDX39BDepositorInsertDDX39B (DDX39B Human)
UseLentiviralTagsV5ExpressionMammalianMutationS296PPromoterE1FaAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNF6_pLX307
Plasmid#98366PurposeLentiviral expression of RNF6DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
JUP_pLX307
Plasmid#98347PurposeLentiviral expression of JUPDepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LIMD1_pLX307
Plasmid#98349PurposeLentiviral expression of LIMD1DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
FERMT1_pLX307
Plasmid#98333PurposeLentiviral expression of FERMT1DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSNK1A1_pLX307
Plasmid#98326PurposeLentiviral expression of CSNK1A1DepositorAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCrysβ:ECFP-LexOP:mCherry-KrasG12V
Plasmid#89888PurposeHuman KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-KRasG12V
UseUnspecifiedTagsmCherryAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKEE401
Plasmid#91715PurposeCRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistanceDepositorTypeEmpty backboneExpressionPlantAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-9:DSP-mEGFP
Plasmid#87424PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human DSPDepositorInsertDSP Homology Arms with linker-mEGFP (DSP Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CHD5-Y619E-FRAG
Plasmid#68871PurposeMammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutationDepositorAvailable SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CHD5-WT-331-672aa
Plasmid#68870PurposeMammalian expression of CHD5 histone binding domain (331-672aa)DepositorAvailable SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHW1133
Plasmid#229869PurposeDestination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCEDepositorTypeEmpty backboneExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp12
Plasmid#218286PurposeMulti-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modulesDepositorInsertHO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-NAT1-C-HA
Plasmid#195178PurposeVector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK237
Plasmid#73339PurposeProduces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusionDepositorInsertcitrate synthase
TagsHis6, Ssp intein, and chitin-binding domainExpressionBacterialPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK let7 G
Plasmid#11330DepositorInsert2 binding sites for let-7a miRNA
UseLuciferaseTagsRr-lucMutationMutation G from figure 4A of paper.Available SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT
Plasmid#204694PurposeThis plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.DepositorTagsmNeonGreenExpressionMammalianMutationdirect fusion of BRD4 and NUTPromoterCMVAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIV515
Plasmid#177703PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1
Plasmid#162164PurposeSelectable homology donor template for Aag2 cell AGO1DepositorInsertsRFP
3xFLAG
UseCRISPR and Cre/Lox; Homology donor templateTagsAGO1 homology and loxPExpressionInsectPromoterAe. aegypti PUb and endogenous AGO1Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP
Plasmid#163030PurposeExpression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianPromoterAlb-AATAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-TRIM28deltaCC1
Plasmid#124955PurposeMammalian expression of Human TRIM28 with deleted CC domainDepositorAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-eno2:Tau-IRES-egfp
Plasmid#112205PurposeUsed to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoterDepositorInsertMAPT (MAPT Human)
UseZebrafish expressionTagseno2 exon 2 translational fusion, ires-egfp at c …Promotereno2 from zebrafishAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only