20,146 results

Showing: 11251 - 11280 of 20146 results

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pMedium-FT-N1

Plasmid

#31911

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pAM1579

Plasmid

#40240

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Km Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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LB1353 (Bcl-2 promoter, no IgH enh)

Plasmid

#15393

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 with full promoter and no IgH enhancers MHS…

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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AICSDP-23:TJP1-mEGFP

Plasmid

#87429

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

TJP1 Homology Arms with mEGFP-linker (TJP1 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pFLAG-CMV-hErk1(K71R)

Plasmid

#49329

Purpose

Expresses kinase deficient FLAG-tagged human Erk1

Depositor

Melanie Cobb

Insert

Erk1 (MAPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K71R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pKM402

Plasmid

#107770

Purpose

Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Inserts

Che9C RecT
TetR (tet repressor)
cat
kan

Expression

Bacterial

Promoter

Cat promoter, Ptet, kan promoter, and pTB21

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PV-NLS-GFP

Plasmid

#17300

Depositor

Anton Bennett

Article

Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

Insert

PV-GFP-NLS (Pvalb Rat)

Tags

Myc and NLS

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs

Plasmid

#109350

Purpose

Wildtype Gαs

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

GαS (GNAS Human)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV_pTp66p51

Plasmid

#78233

Purpose

Expresses HIV reverse transcriptase

Depositor

Christopher Voigt

Article

Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

Inserts

p66
p51
LacI

Expression

Bacterial

Promoter

Const. Promoter (From DNA2.0 Vec) and pTac Minima…

Available Since

Aug. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-Stx7

Plasmid

#45921

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

syntaxin 7 (STX7 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
Fragment1 with TLS2

Plasmid

#196983

Purpose

Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Intermediate fragment for assembly of gRNA-TLS2 construct

Use

Gateway-compatible entry vector

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Tags

V5

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-SorLA-v1

Plasmid

#154892

Purpose

Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

SORL1 (SORL1 Human)

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD1

Plasmid

#114116

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD1

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD1 (SHLD1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1+/mit-2mutAEQ

Plasmid

#45539

Depositor

Javier Alvarez-Martin

Article

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

mit-2mutAEQ

Tags

Mitochondrially targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

Plasmid

#140086

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro-T2A-BFP

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-NOS2Promoter-Luciferase

Plasmid

#19296

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

Insert

NOS2 Promoter (Nos2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A0000

Plasmid

#90997

Purpose

Module A, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CFP-Parkin

Plasmid

#47560

Purpose

CFP tagged Parkin for colocalization study

Depositor

Richard Youle

Article

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Parkin (PRKN Human)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3β-FLAG-CBP-LD-HA

Plasmid

#32906

Depositor

Tso-Pang Yao

Article

Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Insert

C/EBP (Crebbp Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

L1435A/D1436A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPH6

Plasmid

#40315

Depositor

Shaun Lott

Article

Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

Insert

Peptide:N-glycosidase F

Tags

6xHis and OmpA3

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCAGGS_NP_EBOV

Plasmid

#103049

Purpose

Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)

Expression

Mammalian

Mutation

silent mutation (T to C) at position 928 of NP ge…

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmGFP-P2A-K(AAG)20-P2A-RFP

Plasmid

#105689

Purpose

Double fluorescent stalling reporter K(AAG)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Flag Grb10 wt long

Plasmid

#37481

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 (GRB10 Human)

Tags

Flag

Expression

Mammalian

Mutation

isoform a, 594aa

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pDESTsmg

Plasmid

#18878

Depositor

Ted Baker

Article

Goldstone et al Protein Expr Purif. 2008 Jan . 57(1):81-

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-DIO-mTurquoise2

Plasmid

#104059

Purpose

AAV expression of Cre-dependent reporter mTurquoise2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mTurquoise2

Use

AAV

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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20,146 results