162,717 results

1. CRISPR-SB

   Plasmid

   #177936

   Purpose

   Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG5-ABL

   Plasmid

   #31284

   Depositor

   Nora Heisterkamp

   Article

   Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

   Insert

   V-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

   Plasmid

   #101852

   Purpose

   Direct reprogramming of adult fibroblasts to neurons

   Depositor

   Malin Parmar

   Article

   Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

   Insert

   Ascl1, Brn2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6, PGK

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_PGK_antiCD19_synNotch_TetRVP64

   Plasmid

   #79126

   Purpose

   Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   antiCD19_synNotch_TetRVP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMS-II-dCas13-APO1-YTH

   Plasmid

   #204767

   Purpose

   GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   dCas13-NLS-APO1-YTH-HA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1alpha

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Zeo-mEGFP

   Plasmid

   #214145

   Purpose

   Expresses the monomeric EGFP in eukaryotic cells

   Depositor

   Bertrand Collet

   Article

   Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A207K L221K F223R

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPD95_75

   Plasmid

   #1494

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL…

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-SopF

   Plasmid

   #135174

   Purpose

   Expresses an mCherry-SopF fusion in mammalian cells

   Depositor

   Leigh Knodler

   Article

   Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

   Insert

   SopF (STM1239 Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Rubicon

   Plasmid

   #28022

   Depositor

   Qing Zhong

   Article

   Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   3xFlag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19

    Plasmid

    #50005

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR CD19 tandem CAR

    Plasmid

    #113019

    Purpose

    for lentivirus production of CD19 tandem CAR

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Mouse, Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_EF1a_Flag_EGFP

    Plasmid

    #183117

    Purpose

    Expresses flag-tagged EGFP in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-EGFP-NR2b wt

    Plasmid

    #45447

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2B (Grin2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_SFFv_Her2-BFP_RHL027

    Plasmid

    #164823

    Purpose

    Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVSV eGFP dG

    Plasmid

    #31842

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTW2066

    Plasmid

    #236575

    Purpose

    ISYmu1 PDS3 gRNA2 TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PM-B

    Plasmid

    #209876

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mouse E-cadherin GFP (423)

    Plasmid

    #67937

    Purpose

    Mammalian expression of E-cadherin-GFP

    Depositor

    Alpha Yap

    Article

    Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1 mCherry

    Plasmid

    #128073

    Purpose

    puro exchanged to mCherry

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid

    #78311

    Purpose

    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

    Depositor

    Timo Otonkoski

    Article

    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

    Insert

    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. iRFP-PH-PLCdelta1

    Plasmid

    #66841

    Purpose

    expression of near-infrared PI(4,5)P2 biosensor

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    PH-PLCdelta1
    

    Use

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. Cyto-B

    Plasmid

    #209858

    Purpose

    Dimerization dependent fluorescent protein B fused to NES

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pYY166 pcDNA3.4-His-MBP-hAID

    Plasmid

    #231942

    Purpose

    Expression of His-MBP-AID fusion protein in mammalian cells

    Depositor

    Fei-Long Meng

    Article

    Wang et al Cell. 2023 May 11;186(10):2193-2207.e19.

    Insert

    AID (AICDA Human)
    

    Use

    Tags

    His-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti6.3/TO/CD9-GFP

    Plasmid

    #104402

    Purpose

    Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

    Depositor

    Jens Gruber

    Article

    Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBABEpuro-ERBB2

    Plasmid

    #40978

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. pHR_SFFv_4D5-High-CAR_RHL002

    Plasmid

    #164825

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pE-FLP

    Plasmid

    #45978

    Purpose

    Expresses yeast Flp driven by the constitutive promoter pE from phage P2

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Insert

    flp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    flp driven by the constitutive promoter pE from p…

    Promoter

    pE (from phage P2)

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. pPUR-mU6-sgRNA-2XPP7

    Plasmid

    #194504

    Purpose

    Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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