We narrowed to 16,827 results for: IGH@

1. pHH0103_RPA2_RPA-C

   Plasmid

   #109795

   Purpose

   Protein expression and purification of RPA2_RPA-C

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RPA2_RPA-C (RPA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX Fes-SH2

   Plasmid

   #46426

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Fes (FES Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 396-497

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Kif1A(1-489)-DHFR-myc

   Plasmid

   #117833

   Purpose

   Constitutively active Kinesin-3 motor tagged with DHFR and myc

   Depositor

   Thomas Schwarz

   Article

   Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

   Insert

   Kif1A (1-489) (Kif1a Mouse)
   

   Use

   Tags

   DHFR and myc

   Expression

   Mutation

   only aa 1-489 (motor domain)

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-mTDGa.1

   Plasmid

   #81051

   Purpose

   bacterial expression of catalytically dead murine TDG

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TDG (Tdg Mouse)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   asparagine 151 changed to alanine

   Promoter

   T7

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mRuby2-Actin-C-18

   Plasmid

   #55889

   Purpose

   Localization: Actin, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pAG247

   Plasmid

   #33200

   Depositor

   David Bartel

   Article

   Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

   Insert

   LRIG1 (LRIG1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early enhancer/promoter

   Available since

   Jan. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-Elafin

   Plasmid

   #18102

   Depositor

   Ronny Drapkin

   Article

   Labidi-Galy et al Oncogene. 2015 Jan 15;34(3):373-83. doi:

   Insert

   Elafin (PI3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

   Plasmid

   #210504

   Purpose

   expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX p85a(N)-SH2

   Plasmid

   #46464

   Depositor

   Bruce Mayer, Leszek Kotula

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   p85a(N) (PIK3R1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 321-434

   Promoter

   Tac

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103_KPNB1_HEAT

    Plasmid

    #110017

    Purpose

    Protein expression and purification of KPNB1_HEAT

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    KPNB1_HEAT (KPNB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD mApple

    Plasmid

    #91772

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    monomeric Apple
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    R97K, E152S, A180S, V207T compared to mApple (FP …

    Promoter

    araBAD

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mOrange2-Fibrillarin-7

    Plasmid

    #57957

    Purpose

    Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGEX SHP-1(N)-SH2

    Plasmid

    #46495

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(N) (PTPN6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-109

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCKO_EIF3D_sgRNA_1

    Plasmid

    #74187

    Purpose

    lentiviral vector expressing sgRNA targeting EIF3D

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    EIF3D sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Puro-TTL-wt-TTF-1

    Plasmid

    #67657

    Purpose

    To create a dox-on inducible expression of human wt-TTF-1 cDNA in cells

    Depositor

    David Mu

    Article

    Rice et al J Biol Chem. 2013 Jun 7;288(23):16348-60

    Insert

    Thyroid Transcription Factor 1 (NKX2-1 Human)
    

    Use

    Retroviral

    Tags

    N/A

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. miniSOG-Alpha-Actinin-19

    Plasmid

    #57762

    Purpose

    Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pMKO shRNA Bad

    Plasmid

    #17237

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bad shRNA (BAD Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo

    Plasmid

    #179438

    Purpose

    Expresses rTetR-KRAB(rat) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLCKO_EIF3D_sgRNA_4

    Plasmid

    #74188

    Purpose

    lentiviral vector expressing sgRNA targeting EIF3D

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    EIF3D sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p10xUAS-IVS- Syn21-Voltron-p10

    Plasmid

    #119041

    Purpose

    UAS-driven expresion of Voltron in Drosophila

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a_6HT-TBP6.7(R49A)

    Plasmid

    #112726

    Purpose

    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R49A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R49A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

    Plasmid

    #114376

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP, Kv2.1C, and TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330-Flag-dHeFSpCas9

    Plasmid

    #92116

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    Cbh

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCerulean3-Integrin-Beta1-N-18

    Plasmid

    #55423

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Integrin-Beta1 (ITGB1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

25. p1.1-Tr2-eGFP

    Plasmid

    #162782

    Purpose

    Fluorescent reporter for protein expression studies

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-4T-1 3xHA-MKK7a1-EE

    Plasmid

    #47578

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Residues 73-420 of RefSeq sequence. Ser271 to Gl…

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pICE-EGFP-FLAG-Ku70siR-Mut6E

    Plasmid

    #46962

    Purpose

    For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku70 (XRCC6 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Mut6E corresponding to K282E K287E T300E K331E K3…

    Promoter

    CMV Tet

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV_FLAG-NFL(K211TAG)

    Plasmid

    #182660

    Purpose

    Expresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
    

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    K211TAG

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCardinal-Rab4a-7

    Plasmid

    #56174

    Purpose

    Localization: Ras GTPase, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pHH0103_RPA1_RepA-N

    Plasmid

    #110032

    Purpose

    Protein expression and purification of RPA1_RepA-N

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RPA1_RepA-N (RPA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge