3,641 results
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pMT-EGFP-Actin5c
Plasmid#15312DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
dVGlut-Gal80
Plasmid#52555PurposeExpresses Gal80 under vesicular glutamate transporter promoterDepositorExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124434PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in DrosophilaDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
ExpressionInsectAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PUC19_C_tag
Plasmid#186659PurposeEmpty donor plasmid for C-terminal tag of 3xFlag-3xHA.DepositorInsertC-terminal 3xFlag-3xHA Tag
Tags3xFlag-3xHAExpressionInsectAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABR IRFP-Bcd-LEXY
Plasmid#182595PurposeDrosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorAvailable SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB1H2wL-Prd
Plasmid#18040DepositorInsertPaired (prd Fly)
ExpressionBacterialMutationPaired is simply used as an insert between Kpn1 a…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab32
Plasmid#31744DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
ExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB19
Plasmid#194757PurposeEGFP-tagged GluRIIA-Construct for integration at attP sitesDepositorAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB20
Plasmid#194758PurposeALFA-tagged GluRIIA-Construct for integration at attP sitesDepositorAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11
Plasmid#172068PurposeProtein trap plasmid for inserting GFP11 into a phase 1 coding intronDepositorInsertGFP11x7
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT-Aurora B-GFP
Plasmid#24285DepositorAvailable SinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDmelOR
Plasmid#126476PurposeVector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intronDepositorInsertDmelOrco (Orco Fly)
TagsMycExpressionMammalianMutationcodon optimization for H. sapiens; includes a rec…PromoterCMVAvailable SinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
NCD-GFP
Plasmid#24507DepositorAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
K980
Plasmid#129762PurposeExpresses Drosophila Kinesin-1 full-length for microtubule gliding assaysDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::T48-Baz::vhhGFP4
Plasmid#163928PurposeExpression of GrabFP-A-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTub-Wg
Plasmid#64321PurposeExpresses Drosophila Wingless protein under the tubulin promotorDepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAFW-Ago2
Plasmid#50554PurposeExpression of FLAG tagged Ago2 in S2 cellDepositorAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
908H
Plasmid#160240PurposeTetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attBDepositorInsertsTet Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
TagsT2A GFPExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarG
Plasmid#31221DepositorInsertCD4-tdGFP (CD4 Aequorea victoria, Fly, Human)
TagsCD4 and EGFP/GFPExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …Available SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUAST td tomato Rab5 WT
Plasmid#58446Purposeexpression of drosophila td tomato Rab5DepositorAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
ninaE-norpA
Plasmid#26854DepositorAvailable SinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAWG-dCas9-VPR
Plasmid#63802PurposeSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vectorDepositorInsertSP-dCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionInsectPromoteract5cAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRB17
Plasmid#52527Purposeserves as template for PCR-mediated fusion of U6-promoter with sgRNADepositorInsertDrosophila U6C promoter
UseCRISPR; Blunt cloning kitTagsT7-promoterExpressionInsectAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
gRNAy
Plasmid#168294PurposeExpresses gRNA targeting the yellow gene in DrosophilaDepositorInsertU6.3-gRNA[y]-scaffold
ExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-y1sgRNA-Cas9
Plasmid#49331PurposeExpresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertsUseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS Exon Vector
Plasmid#69884PurposeExpression plasmid for expressing circular RNAs of a desired sequence in DrosophilaDepositorAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD7
Plasmid#73916PurposegRNA vector for AsCpf1DepositorInserttRNA:hAsCpf1-gRNA:tRNA
UseCRISPRExpressionInsectPromoterdU6:3Available SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
KHBD00532
Plasmid#39630DepositorAvailable SinceAug. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKC52
Plasmid#226780PurposeExpresses Drosophila UNC-104(R348G) in insect cellsDepositorInsertDrosophila unc-104 (R348G) (unc-104 Fly)
TagssfGFP and 2xStrepIIExpressionInsectMutationchanged Arginine 348 to GlycineAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKC50
Plasmid#226778PurposeExpresses Drosophila UNC-104(V6M) in insect cellsDepositorInsertDrosophila unc-104 (V6M) (unc-104 Fly)
TagssfGFP and 2xStrepIIExpressionInsectMutationchanged Valine 6 to MethionineAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pUAST_NSlmb-vhhGFP4
Plasmid#35575DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
ExpressionInsectAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
DmTmem63
Plasmid#136598PurposeExpresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cellsDepositorInsertDmTMEM63
ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w2-mutOdd
Plasmid#18044DepositorInsertmutant Odd Skipped
ExpressionBacterialMutationA mutant version of the zinc finger protein Odd S…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20X-UAS-ADARcd
Plasmid#81173Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertUAS-ADARcd
ExpressionInsectPromoterUASAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
Plasmid#217511PurposeKD Recombinase dependent conditional smGdP-10XHA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
TagssmGdP-10XHAExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
KRigor980
Plasmid#129763PurposeExpresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfacesDepositorAvailable SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmBelle_B
Plasmid#145950PurposeBacterial Expression of DmBelleDepositorInsertDmBelle (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
Plasmid#217512PurposeKD Recombinase dependent conditional smGdP-10XOllas cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
TagssmGdP-10XOllasExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-Dm-miR-92a
Plasmid#22767DepositorInsertmicro RNA 92a (mir-92a Fly, primary transcript)
UseDrosophila expressionAvailable SinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
TagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559Available SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBDP
Plasmid#17566DepositorInsertmini w+
ExpressionInsectAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ftz-M3
Plasmid#11245DepositorInsertFtz-M3
Tags3 MS2 hairpinsExpressionBacterialAvailable SinceJan. 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
doraWT_rescue_construct
Plasmid#190609PurposePuromycin-selectable expression of Dora (CG34401) in Drosophila S2 cellsDepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-Venus
Plasmid#58328PurposeDestination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genomeDepositorInsertCad-Venus
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only
