176,436 results

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pCLBW cox8 EGFP mCherry

Plasmid

#78520

Purpose

Fluorescent mitophagy reporter

Depositor

David Chan

Publication

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

none

Use

Retroviral

Tags

cox8 EGFP mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Nirao Shah, Jim Wells

Publication

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has service

AAV1, AAV5, and AAV8

Inserts

Caspase 3 (CASP3 Human, Synthetic)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

74 Articles

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pEGFP-IQGAP1

Plasmid

#30112

Depositor

David Sacks

Publication

Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

Insert

IQGAP1 (IQGAP1 Human)

Tags

tandem EGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAAV-Ef1a-DIO EYFP

Plasmid

#27056

Depositor

Karl Deisseroth

Has service

AAV1, AAV2, AAV5, AAV9, and AAV Retrograde

Insert

EYFP

Use

AAV

Expression

Mammalian

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

203 Articles

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pENTR4-FLAG (w210-2)

Plasmid

#17423

Purpose

Gateway entry vector for N-term FLAG tag

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

FLAG

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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p3258 pCMV hUBC9 wt HA

Plasmid

#14438

Depositor

Peter Howley

Publication

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 (UBE2I Human)

Tags

HA

Expression

Mammalian

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pX330 p53

Plasmid

#59910

Purpose

pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Publication

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting p53 (Trp53 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Publication

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP

Expression

Mammalian

Available since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

18 Articles

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IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Publication

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Human, Mouse)

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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HA-hULK1

Plasmid

#31963

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (ULK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pCMV-myc-Atg7

Plasmid

#24921

Depositor

Toren Finkel

Publication

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

Atg7 (ATG7 Human)

Tags

Myc

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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FUW-tetO-hSOX2

Plasmid

#20724

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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HER2 CA (V659E)

Plasmid

#16259

Depositor

Mien-Chie Hung

Publication

Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

Insert

HER2 (ERBB2 Human)

Expression

Mammalian

Mutation

Constitutively active: V659E

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pSico PGK puro

Plasmid

#11586

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Publication

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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p53-GFP

Plasmid

#11770

Depositor

Geoff Wahl

Insert

p53 (TP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

72R variant of p53

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pGTR

Plasmid

#63143

Purpose

PCR template to construct polycistronic tRNA-gRNA

Depositor

Yinong Yang

Publication

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

gRNA-tRNA fusion

Use

CRISPR and Synthetic Biology

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Publication

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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pCDNA-3xHA-hTERT

Plasmid

#51637

Purpose

Mammalian expression of 3xHA tagged human TERT, N-terminal.

Depositor

Steven Artandi

Publication

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

TERT (TERT Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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EMTB-3XGFP

Plasmid

#26741

Depositor

William Bement

Publication

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

3x EGFP and Myc

Expression

Mammalian

Mutation

Only contains the microtubule binding-domain of e…

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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pcDNA3-FLAG-DDB1

Plasmid

#19918

Depositor

Yue Xiong

Publication

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAMPK alpha2 K45R

Plasmid

#15992

Depositor

Morris Birnbaum

Publication

Mu et al Mol Cell. 2001 May . 7(5):1085-94.

Insert

AMPK alpha2 (Prkaa2 Rat)

Tags

Myc

Expression

Mammalian

Mutation

K45R

Promoter

CMV

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GFP-P4M-SidM

Plasmid

#51469

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Publication

Hammond et al J Cell Biol. 2014 Apr 7.

Insert

P4M-SidM(546-647)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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4xCSL-luciferase

Plasmid

#41726

Depositor

Raphael Kopan

Publication

Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

Insert

4xCSL binding sites

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains a multimerized high affinity CSL binding…

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pCMVHA EED wt

Plasmid

#24231

Purpose

Mammalian expression of human EED with HA tag

Depositor

Kristian Helin

Publication

Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

Insert

EED (EED Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCS2-MT GLI2 FL

Plasmid

#17648

Depositor

Erich Roessler

Publication

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Promoter

CMV IE94

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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dxCas(3.7)-VPR

Plasmid

#108383

Purpose

Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

dxCas(3.7)-VPR

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCRIS-PITChv2-dTAG-BSD (BRD4)

Plasmid

#91795

Purpose

PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Publication

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

FKBP_F36V-2xHA-P2A-BSD

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Publication

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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FUW-tetO-hOCT4

Plasmid

#20726

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

OCT4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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