163,606 results

1. GFP-RNF2

   Plasmid

   #126544

   Purpose

   Expresses GFP-tagged RNF2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RING1B (RNF2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-flag-GFP-JAM-B

   Plasmid

   #205191

   Purpose

   This vector encodes of a synCAM (JAM-B) and GFP

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM JAM-B and GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-NG

   Plasmid

   #138566

   Purpose

   Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-CAG-flex-iGABASnFR2-WPRE

   Plasmid

   #218880

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   CAG

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSUPER-scramble

   Plasmid

   #118349

   Purpose

   This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   scrambled shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-hSyn-EGFP

   Plasmid

   #114215

   Purpose

   Lentiviral vector (control) that targets expression of EGFP to neurons

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   human synapsin (hSyn)

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-FGFR2c-V5/HIS

   Plasmid

   #201107

   Purpose

   expression of human FGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.18 CMV-Luc

   Plasmid

   #100984

   Purpose

   CMV luciferase reporter - firefly luciferase driven by CMV promoter

   Depositor

   Lee Helman

   Article

   Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

   Insert

   CMV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCKmatBC

   Plasmid

   #138587

   Purpose

   matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   matB contains native GUG start codon

   Promoter

   Plac promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Eric Shusta, Sean Palecek

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF142_U6-sgRNA

    Plasmid

    #225960

    Purpose

    U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    U6-sgRNA (recipient)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PRDX1A-c001

    Plasmid

    #175473

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX1 (PRDX1 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE-tdTomato

    Plasmid

    #50798

    Purpose

    Expression of tdTomato under the control of the TRE-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    TREtight driven tdTomato fluorescent protein
    

    Use

    Mutli-species

    Tags

    Expression

    Mutation

    Promoter

    TREtight

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. p146

    Plasmid

    #186178

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 146
    

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA Myc DBC1

    Plasmid

    #35096

    Depositor

    Osamu Hiraike

    Article

    Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

    Insert

    deleted in breast cancer 1 (CCAR2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-myc-Skp2

    Plasmid

    #19947

    Depositor

    Yue Xiong

    Article

    Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

    Insert

    S-phase kinase-associated protein 2 (p45) (SKP2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid

    #159149

    Purpose

    expresses His-tagged HIV reverse transcriptase and untagged HIV protease

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Inserts

    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    HIV-1 group M/subtype B – BH10 strain

    Promoter

    Available Since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

    Plasmid

    #228721

    Purpose

    Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. A*11:01:01:01

    Plasmid

    #203276

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*11:01:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTREGFP2

    Plasmid

    #22450

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    EGFP (eGFP )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MAC_N_IRF1

    Plasmid

    #167760

    Purpose

    Expresses IRF1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    IRF1 (IRF1 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-Moesin-C-14

    Plasmid

    #55103

    Purpose

    Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Moesin (MSN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pENTR-SYNZIP18

    Plasmid

    #80672

    Purpose

    Encodes the synthetic coiled coil peptide SYNZIP18

    Depositor

    Amy Keating

    Article

    Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

    Insert

    SYNZIP18
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

    Plasmid

    #193728

    Purpose

    Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

    Depositor

    Colin Akerman

    Article

    Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

    Insert

    ClopHensorN
    

    Use

    AAV

    Tags

    Strep-II (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p156

    Plasmid

    #186179

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 156
    

    Use

    Synthetic Biology and Unspecified; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p136

    Plasmid

    #186177

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 136
    

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MCP-MSN

    Plasmid

    #210704

    Purpose

    This plasmid express EF1a promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAB076i3

    Plasmid

    #79198

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-mito-ApplePy1Highest

    Plasmid

    #239074

    Purpose

    Mammalian expression of mitochondria-targeted highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    ApplePy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge