165,744 results

1. PiggyBac PlayBack

   Plasmid

   #203312

   Purpose

   To all for PiggyBac gene insertion into Genome

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVET-IRES-GFP

   Plasmid

   #107139

   Purpose

   A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

   Depositor

   Aki Manninen

   Article

   Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICH41331::pU6cm-pegR::EURb7

   Plasmid

   #227695

   Purpose

   Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   Altered epegRNA backbone with cloning sites for gRNA and RTT.
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   U6 composite

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST53-LRRK2-WT

   Plasmid

   #25044

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB4581

   Plasmid

   #171124

   Purpose

   A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

   Insert

   3_sAID
   

   Use

   Other

   Mutation

   non applicable

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. psfGFP-LAMP1-mCherry (pHLARE)

   Plasmid

   #164477

   Purpose

   Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

   Depositor

   Diane Barber

   Article

   Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   mCherry and superfolder GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-MT GLI2 delta N

   Plasmid

   #17649

   Depositor

   Erich Roessler

   Article

   Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

   Insert

   GLI2 (GLI2 Human)
   

   Tags

   6x Myc

   Expression

   Mammalian

   Mutation

   Missing 328 N-terminal amino acids

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-Guide-Puro

    Plasmid

    #86708

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Christoph Bock

    Article

    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-hOCT3/4

    Plasmid

    #27076

    Purpose

    Non-integrating (episomal) expression of human OCT3/4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    William Hahn, John Doench, David Root

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLminP_Luc2P_RE27

    Plasmid

    #90369

    Purpose

    Type II Interferon - STAT1/STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    STAT1

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCF159_BRL

    Plasmid

    #225962

    Purpose

    CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-BRL (env protein)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Article

    Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Expression

    Bacterial

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EGFP-IRES-puro

    Plasmid

    #128652

    Purpose

    mammalian expression of EGFP with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-Tol2 transposase

    Plasmid

    #158774

    Purpose

    Expression of Tol2 transposase

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    Tol2 tranposase
    

    Expression

    Mammalian

    Mutation

    Tol2 codon usage frequency well suitable for muri…

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAD100-CHIP-myc-His

    Plasmid

    #236054

    Purpose

    Mammalian expression of human CHIP with a C-terminal myc-His tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    CHIP (STUB1 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST53-LRRK2-G2019S

    Plasmid

    #25045

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Glycine 2019 mutated to Serine

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hsyn-ASAP5-Kv2.1-WPRE

    Plasmid

    #225707

    Purpose

    Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Has Service

    AAV1

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Promoter

    human synapsin

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEvol-pAzFRS.2.t1

    Plasmid

    #73546

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.2.t1
    pAzFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid

    #72486

    Purpose

    Express Luc2 and tdTomato under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDB5050

    Plasmid

    #171126

    Purpose

    A plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

    Insert

    OsTIR1
    

    Use

    Other

    Mutation

    non applicable

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDY1440 STITCHR GFP NOLC1 payload

    Plasmid

    #234829

    Purpose

    GFP cargo with 50bp NOLC homology arms

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    copGFP
    

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEGFP-GFP11-Clathrin light chain

    Plasmid

    #70217

    Purpose

    Expresses GFP11-clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11-Clathrin light chain (CLTA Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLC-ZsGreen-P2A-Puro

    Plasmid

    #124302

    Purpose

    Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    ZsGreen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. His6-TEV-CRBN-midi

    Plasmid

    #215330

    Purpose

    CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

    Depositor

    Alessio Ciulli

    Article

    Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

    Insert

    CRBN (CRBN Human)
    

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 41-187 followed by a Gly-Ser…

    Promoter

    lacI

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMOS031: superecliptic pHluorin (endoplasmic reticulum)

    Plasmid

    #163051

    Purpose

    superecliptic pHluorin (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    superecliptic pHluorin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PtipA; crRNA under control of PermE*

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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