Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 39,681 results for: Eras

Showing: 1641 - 1680 of 39681 results

  1. p663-UBC-miniTurbo-V5-CAMK1G_IDG-K

    Plasmid
    #158168
    Purpose
    Gateway destination clone of CAMK1G (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CAMK1G (CAMK1G Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158168-plasmid-map-sequence-id-309974

    Enlarge

  2. p667-UBC-BRSK2-V5-miniTurbo_IDG-K

    Plasmid
    #135252
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    BRSK2-V5-miniTurbo (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135252-plasmid-map-sequence-id-269118

    Enlarge

  3. p663-UBC-miniTurbo-V5-BRSK2_IDG-K

    Plasmid
    #135271
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-BRSK2 (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135271-plasmid-map-sequence-id-269110

    Enlarge

  4. p667-UBC-BRSK2-V5_IDG-K

    Plasmid
    #135254
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    BRSK2-V5 (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135254-plasmid-map-sequence-id-268566

    Enlarge

  6. pIhh4.5k-Luc

    Plasmid
    #53603
    Purpose
    Reporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pMiR-HPV16-E1-m5

    Plasmid
    #53702
    Purpose
    Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site
    Depositor
    Edward Chan
    Article
    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10
    Insert
    HPV16-E1(partial)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutation in miR-375 binding site m5 (refer to cit…
    Promoter
    Available since
    June 26, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pMiR-HPV16-E1-m4

    Plasmid
    #53701
    Purpose
    Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site
    Depositor
    Edward Chan
    Article
    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10
    Insert
    HPV16-E1(partial)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutation in miR-375 binding site m4 (refer to cit…
    Promoter
    Available since
    June 26, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pMiR-HPV16-E1-m3

    Plasmid
    #53700
    Purpose
    Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site
    Depositor
    Edward Chan
    Article
    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10
    Insert
    HPV16-E1(partial)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutation in miR-375 binding site m3 (refer to cit…
    Promoter
    Available since
    June 26, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pMiR-HPV16-E7-m2

    Plasmid
    #53698
    Purpose
    Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site
    Depositor
    Edward Chan
    Article
    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10
    Insert
    HPV16-E7
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutation in miR-375 binding site m2 (refer to cit…
    Promoter
    Available since
    June 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pMiR-HPV16-E7-m1

    Plasmid
    #53697
    Purpose
    Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site
    Depositor
    Edward Chan
    Article
    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10
    Insert
    HPV16-E7
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutation in miR-375 binding site m1 (refer to cit…
    Promoter
    Available since
    June 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pHAGE-CMV-Flag-CDK13_IDG-K

    Plasmid
    #135276
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    135276-plasmid-map-sequence-id-267149

    Enlarge

  16. p663-UBC-TurboID-V5-CDK13_IDG-K

    Plasmid
    #135267
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135267-plasmid-map-sequence-id-269144

    Enlarge

  17. YIP sHRPa-Aga1P

    Plasmid
    #73151
    Purpose
    Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-Aga1P
    Use
    Tags
    V5 tag
    Expression
    Yeast
    Mutation
    Promoter
    glyceraldehyde-3-phosphate dehydrogenase GPD prom…
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73151-plasmid-map-sequence-id-134538

    Enlarge

  25. PYL1_nTEVp

    Plasmid
    #119213
    Purpose
    Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nTEVp in sufion with PYL1
    Use
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119213-plasmid-map-sequence-id-232030

    Enlarge

  26. ABI_cTEVp

    Plasmid
    #119214
    Purpose
    Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cTEVp in fusion with ABI
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119214-plasmid-map-sequence-id-231821

    Enlarge

  28. pSNRW-Z5-Nluc

    Plasmid
    #174487
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z5-Nluc
    Use
    Tags
    6x Histidine tag (N terminal on insert)
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174487-plasmid-map-sequence-id-345291

    Enlarge

  30. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid
    #170578
    Purpose
    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDKL5 (CDKL5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    E854G- please see depositor comments
    Promoter
    Ubiquitin
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    170578-plasmid-map-sequence-id-340993

    Enlarge

  34. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Article
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Use
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

    Enlarge

  35. pPRIME-TET-GFP-FF3

    Plasmid
    #11662
    Purpose
    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase
    Depositor
    Stephen Elledge
    Article
    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1
    Insert
    FF3
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 1, 2007
    Availability
    Academic Institutions and Nonprofits only
    11662-plasmid-map-sequence-id-7008

    Enlarge

  36. pCLucIPZ

    Plasmid
    #53222
    Purpose
    Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest
    Depositor
    Thorsten Stiewe
    Article
    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1
    Inserts
    Cypridina luciferase
    non-silencing shRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53222-plasmid-map-sequence-id-342548

    Enlarge

  39. p667-UBC-COQ8A-V5-miniTurbo_IDG-K

    Plasmid
    #189885
    Purpose
    Gateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    COQ8A (COQ8A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189885-plasmid-map-sequence-id-374487

    Enlarge

Showing: 1641 - 1680 of 39681 results