We narrowed to 4,630 results for: lum

1. pCS2-mRuby-Rab8a

   Plasmid

   #194337

   Purpose

   Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Insert

   Rab8 (RAB8A Canis lupus)
   

   Use

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-mTcte1-PA

   Plasmid

   #176469

   Purpose

   Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   t-complex-associated testis expressed 1 (Tcte1 Mouse)
   

   Use

   Tags

   PA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-SARS-CoV-2 Nsp3TM

   Plasmid

   #204206

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Use

   Tags

   FLAGx1

   Expression

   Mammalian

   Mutation

   none, from obtained clone

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-X1-Neo-GFP-ATL1

   Plasmid

   #109018

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL1 (ATL1 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EIF-1a promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mADAM10 E385A in pQCXIP cFLAG

   Plasmid

   #19137

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM10 (Adam10 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E385A

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

6. mCherry-FKBP-SAC1-HLx8

   Plasmid

   #108136

   Purpose

   Recruitable SAC1 with extended linker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-Flex-mRuby3-2A

   Plasmid

   #203846

   Purpose

   Neuron specific expression of a Cre-dependent mRuby3

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MO70-mJP-4

   Plasmid

   #79598

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

   Insert

   Junctophilin-4 (Jph4 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pEX-CMV-SP-CFP-STM2(15-746)

   Plasmid

   #18863

   Depositor

   Tobias Meyer

   Article

   Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

   Insert

   Stromal Interaction Molecule 2 (STIM2 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP inserted between signal peptide and residues …

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

    Plasmid

    #72888

    Purpose

    fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Insert

    Gaussia luciferase, Channelrhodopsin-2, EYFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sfGFP-SasG(8G)-mCherry

    Plasmid

    #206950

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (8G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (8G)-mCherry
    

    Use

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI-Neo-gp78/JM20

    Plasmid

    #13303

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV6- Entry-RPL26DC-DDK

    Plasmid

    #133867

    Purpose

    Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26DC-myc-FLAG (RPL26 Human)
    

    Use

    Tags

    Myc-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MPRA_minCMV+GFP

    Plasmid

    #204866

    Purpose

    Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

    Depositor

    Philipp Maass

    Article

    Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

    Insert

    minCMV:GFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mADAM10 deltaMP in pQCXIP cFLAG

    Plasmid

    #19138

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM10 (Adam10 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 56-457

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

16. mEmerald-H2B-6

    Plasmid

    #54111

    Purpose

    Localization: Nucleus/Histones, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. 1M-Tm-antimCherry

    Plasmid

    #206952

    Purpose

    Mammalian expression of 1M-Tm EMcapsulins targeting mCherry

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, TmEnc, antimCherry intrabody (LaM-4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1M-Qt-antiGFP

    Plasmid

    #206951

    Purpose

    Mammalian expression of 1M-Qt EMcapsulins targeting GFP

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-BcRF1-2xStrep

    Plasmid

    #138436

    Purpose

    Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDECKO-mCherry MALAT1_Enhancer.1

    Plasmid

    #78536

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-X1-Neo-GFP-ATL2-R244Q

    Plasmid

    #109023

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-R244Q (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Arg244Gln mutation results in dimerization defect

    Promoter

    EIF-1a promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA5FRT/TO-Strep/HA-UBE2QL1

    Plasmid

    #124665

    Purpose

    Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 PA-mCit

    Plasmid

    #113443

    Purpose

    ProteinA-mCitrine control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-FKBP-SAC1-HLx10

    Plasmid

    #108137

    Purpose

    Recruitable SAC1 with extended linker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520):[EAAAR]10:SACM1L(521-587)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-synp-F-H2B-GCaMP6f

    Plasmid

    #102994

    Purpose

    Expresses histone fused GCaMP6f under the synapsin promoter

    Depositor

    David Cox

    Article

    Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

    Insert

    H2B-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA4/TO-UL87-2xStrep

    Plasmid

    #138434

    Purpose

    Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87 (UL87 HCMV (HHV-5))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGP-CMV-GCaMP3-44

    Plasmid

    #91780

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-44
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDECKO-mCherry MALAT1_Exon.1

    Plasmid

    #78540

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CFF-EGFP

    Plasmid

    #86061

    Purpose

    A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains luminal and transmembrane domains of CD8…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSicoR-Ef1a-mCh-GFPi

    Plasmid

    #31849

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA5FRT/TO-Strep/HA-UBE2Q2

    Plasmid

    #124668

    Purpose

    Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2Q2 (UBE2Q2 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSicoR-Ef1a-mCh-Puro-Luci

    Plasmid

    #31850

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    Luciferase RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #104984

    Purpose

    Expresses ST-Renilla reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMM1157

    Plasmid

    #112534

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    luxCDABE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL(HrtO) and ProD

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMAL-c2X-ORF24-NTD

    Plasmid

    #138465

    Purpose

    Expresses MBP-tagged ORF24-NTD in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Amino acids 1-201

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX4T1-ORF24-NTD

    Plasmid

    #138464

    Purpose

    Expresses GST-tagged ORF24-NTD in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 1-201

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA4/TO-ORF24-3xFLAG

    Plasmid

    #138423

    Purpose

    Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLKO.1-blast-cfTuba_1

    Plasmid

    #26717

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Dog Tuba RNAi

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

41. p35S::IRE1a

    Plasmid

    #135232

    Purpose

    constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    IRE1a (IRE1A Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCI-Neo-gp78/2853

    Plasmid

    #11435

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLKO.1-blast-cfTuba_2

    Plasmid

    #26718

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Canine Tuba_2 RNAi

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

44. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUC19 PKC epsilon

    Plasmid

    #8442

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Mar 25;268(9):6090-6.

    Insert

    Mouse Protein Kinase C epsilon (Prkce Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

46. mCherry-FKBP-SAC1C389S-HLx8

    Plasmid

    #108138

    Purpose

    Recruitable inactive SAC1 with extended linker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid

    #138208

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub(T/A)-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLTR PKC zeta

    Plasmid

    #8421

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C zeta (Prkcz Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

49. pPK-351

    Plasmid

    #157921

    Purpose

    pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

    Depositor

    Phillip Kyriakakis

    Article

    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

    Inserts

    PIF3-MTAD
    IRES
    PhyB(1-621)-SV40NLS-Gal4DBD
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    HA tag and SV40NLS

    Expression

    Mammalian

    Mutation

    pif3 1-523

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. p6H-SUMO3-ORF24-NTD-Strep

    Plasmid

    #138467

    Purpose

    Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    SUMO3 and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Amino acids 2-201

    Promoter

    T7

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge