We narrowed to 3,430 results for: Agt

1. pTK73.ChrII_ttTi5605.sgRNA

   Plasmid

   #194058

   Purpose

   sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ChrII_ttTi5605 sgRNA
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE pGK hNLRP3-mNG

   Plasmid

   #218643

   Purpose

   Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   pGK

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACE2 g4 + Cas9

   Plasmid

   #153014

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CAG-EGFP-LacZshRNA

   Plasmid

   #73977

   Purpose

   LacZshRNA (mir155 backbone) was inserted into the 3'UTR of EGFP.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the LacZ gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1P shNFS1_1

   Plasmid

   #102963

   Purpose

   Suppression of NFS1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shNFS1_1 (NFS1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHX_gRNA_hPAX7_6-9S

   Plasmid

   #160453

   Purpose

   sgRNA targeting human PAX7 3' UTR with 17bp spacer

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7_6-9S
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCY_gRNA_hPAX7-Pro_C4

   Plasmid

   #160455

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221 hNLRP3-mNG-STOP

   Plasmid

   #218644

   Purpose

   Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Gateway entry

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

   Plasmid

   #84249

   Purpose

   Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgSV40
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FUGW-H1-Syndecan-1 shRNA (ORF)

    Plasmid

    #40622

    Depositor

    Sally Temple

    Article

    Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

    Insert

    Syndecan-1 shRNA(ORF) (Sdc1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

11. PX459-CDKN1A

    Plasmid

    #217457

    Purpose

    For CDKN1A (p21) knockout

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for CDKN1A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-U6-AasgRNA3.8

    Plasmid

    #121958

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, short version
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTFE3.1.0-gDNA

    Plasmid

    #112473

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3

    Depositor

    Kevin White

    Insert

    TFE3 (TFE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CAG-mCherry-LacZshRNA

    Plasmid

    #73978

    Purpose

    LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the LacZ gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEN_TmiRc3_shURB5

    Plasmid

    #81066

    Purpose

    Gateway entry vector encoding shRNA to UBR5

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    shRNA targeting UBR5
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSuper-PICK1 shRNA

    Plasmid

    #67522

    Purpose

    Expresses shRNA against PICK1

    Depositor

    Victor Anggono, Richard Huganir

    Article

    Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

    Insert

    PICK1 (Pick1, Pick1 Rat, Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJZC523

    Plasmid

    #62320

    Purpose

    sgRNA for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSDMA66

    Plasmid

    #128355

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMP-Suv39H2_2

    Plasmid

    #36345

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Suv39H2 (SUV39H2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pSDMA67

    Plasmid

    #128356

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. phU6 ST

    Plasmid

    #188712

    Purpose

    sgRNA plasmid encoding hU6 promoter driving an anti-safe targeting sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    Safe Targeting sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmU6 ST

    Plasmid

    #188713

    Purpose

    sgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    Safe Targeting sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p7SK ST

    Plasmid

    #188714

    Purpose

    sgRNA plasmid encoding 7SK promoter driving an anti-safe targeting sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    Safe Targeting sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. phH1 ST

    Plasmid

    #188715

    Purpose

    sgRNA plasmid encoding hH1 promoter driving an anti-safe targeting sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    Safe Targeting sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMLS719

    Plasmid

    #188894

    Purpose

    miniMos Arm sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(K09B11.12)::miniMos Arm sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSP64T-ythdf2-3xflag

    Plasmid

    #164489

    Purpose

    Expresses zebrafish ythdf2 with 3x flag tag, for mRNA injection

    Depositor

    Antonio Giraldez

    Article

    Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

    Insert

    ythdf2 (ythdf2 Zebrafish)
    

    Use

    Production of mrna for zebrafish injection.

    Tags

    3x-flagtag

    Expression

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJAID6A

    Plasmid

    #165046

    Purpose

    Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJAID8Z104

    Plasmid

    #165056

    Purpose

    Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PX458_NR2F6_2

    Plasmid

    #86343

    Purpose

    Encodes gRNA for 3' target of human NR2F6

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NR2F6 (NR2F6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge