Addgene

We narrowed to 15,192 results for: genome

Showing: 1681 - 1720 of 15192 results

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CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#194715

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSDM177

Plasmid

#194476

Purpose

pHERD30T-crRNA(Lse)_V3

Depositor

Joseph Bondy-Denomy

Article

Guan et al Nat Microbiol. 2022 Oct 31. pii: 10.1038

Insert

crRNA(Lse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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act5c-LbCas12a

Plasmid

#140620

Purpose

Ubiquitous expression of LbCas12a in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Insert

LbCas12a

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEA186

Plasmid

#187874

Purpose

Empty pVS1-based INTEGRATE vector with mCherry cargo

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA6 1397N

Plasmid

#221934

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

DddA: Q1310R, S1330I, T1380I

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE 1397C

Plasmid

#221936

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

TALE: Q231R, W232G, S233A

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE 1397N

Plasmid

#221937

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

TALE: Q231R, W232G, S233A

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE 1397C

Plasmid

#221931

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddAtox 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FusX DdCBE 1397N

Plasmid

#221932

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddAtox 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL2315

Plasmid

#187876

Purpose

Expresses Cre recombinase.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

J23107

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP

Plasmid

#32628

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Sce

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pKL2310

Plasmid

#187875

Purpose

Empty pVS1-based INTEGRATE vector with loxP cargo

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

Plasmid

#32149

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

FRT> STOP STOP FRT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pJFRC165-20XUAS-IVS-R::PEST

Plasmid

#32142

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

R::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCJ162

Plasmid

#175055

Purpose

lox66-72 flanked gentamycin resistance cassette integrates into aquI integration site

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

lox66::gmR::lox71

Use

Unspecified

Tags

Expression

Mutation

Promoter

n/a

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFRm43GW

Plasmid

#133748

Purpose

Three-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pKTol2C-EGFP

Plasmid

#85598

Purpose

Tol2 transposon with EGFP driven by minimal-CAGs promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

mini-CAG

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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enOgeuIscB

Plasmid

#219636

Purpose

CMV-SV40 NLS-enOgeuIscB-nucleoplasmin NLS

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Insert

CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9HC

Plasmid

#43945

Depositor

Jin-Soo Kim

Article

Cho et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Cas9

Use

CRISPR

Tags

HA epitope and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pFF753

Plasmid

#61453

Purpose

IPTG-inducible msd(wt)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFF530

Plasmid

#61447

Purpose

IPTG-inducible msd_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFH14

Plasmid

#125799

Purpose

Level0 SaCas9, Arabidopsis codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SaCas9, Arabidopsis codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAC200

Plasmid

#187911

Purpose

psac200-crispey-bar-vector

Depositor

Hunter Fraser

Article

Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC161-20XUAS-IVS-KD::PEST

Plasmid

#32140

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

KD::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3nK_ext_AC

Plasmid

#108686

Purpose

Plasmid for assembly of donor DNA templates consisting of two expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3nK_ext_AD

Plasmid

#108687

Purpose

Plasmid for assembly of donor DNA templates consisting of three expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFG7m34GW

Plasmid

#133747

Purpose

Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH47

Plasmid

#125812

Purpose

Level0 LbCas12a, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF745

Plasmid

#61450

Purpose

IPTG-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA with U6 promoter

Plasmid

#48962

Purpose

to drive the sgRNA expression under a U6 promoter

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKUb-Tol2

Plasmid

#85601

Purpose

Source of Tol2 transposase driven by a human ubiquitin promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

human Ubiquitin

Available since

Feb. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFD8

Plasmid

#140619

Purpose

Expression of one of several LbCas12a crRNAs in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6:3

Available since

Oct. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEA183

Plasmid

#187873

Purpose

Expresses eGFP under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Agrobacterium virB promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKHR4

Plasmid

#74592

Purpose

an ampR plasmid built from pBluescript: the multiple cloning site (mcs) flanked by inverted head-to-head oriented I-SceI sites. Genbank accession number is KU144822.

Depositor

David Grunwald

Article

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV578

Plasmid

#108279

Purpose

RMCE vector containing inverted PhiC31 attB sites

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attB sites

Use

CRISPR; Rmce

Tags

Expression

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41308::hCas9

Plasmid

#49770

Purpose

Level 0 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

hCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF838

Plasmid

#61457

Purpose

aTc-inducible SCRIBE_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_lacZ(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pTetO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CD4d3+4-bio

Plasmid

#32402

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Type

Empty backbone

Use

Tags

biotinylation peptide and ratCd4d3+4

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJH1320

Plasmid

#36915

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

Cre recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7tyrgRNA

Plasmid

#46761

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

tyr gRNA (tyr Zebrafish)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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