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We narrowed to 4,183 results for: 256

Showing: 1721 - 1760 of 4183 results

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pBa-FRB-3myc-Rab5a

Plasmid

#64209

Purpose

Rab5a with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab5a (Rab5a Mouse)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

Promoter

chicken Beta Actin

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 E76A

Plasmid

#8321

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

E76A (activated mutant)

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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plKO.1-DNAJC5-ShRNA

Plasmid

#205729

Purpose

Knockdown of DNAJC5

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

shRNA targeting DNAJC5 (DNAJC5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pFLAG W96A-GLTP

Plasmid

#170741

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

GLTP (GLTP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

W96A

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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B2387_mEGFP-FKBP2x

Plasmid

#216216

Purpose

Expresses mEGFP(A206K) fused to FKBP-FKBP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

mEGFP-FKBP-FKBP (FKBP1A Human)

Use

Tags

EGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5 (WT)-HaloTag

Plasmid

#205710

Purpose

Mammalian expression of DNAJC5 (WT)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJ128

Plasmid

#19962

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Use

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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keratin 8 (krt8)-H2B-RFP

Plasmid

#105955

Purpose

transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

krt8 (KRT8 Human)

Use

Tags

RFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67295

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_PRKACA_C1+C2

Plasmid

#110027

Purpose

Protein expression and purification of PRKACA_C1+C2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PRKACA_C1+C2 (PRKACA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- WTN23C11orf83-GFP

Plasmid

#65845

Purpose

Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT Transmembrane

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T2 SHP2 E76A

Plasmid

#8324

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

E76A (activated mutant)

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE

Plasmid

#67292

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Myt1L (Myt1l Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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FN 108

Plasmid

#50495

Depositor

Kenneth Yamada

Article

Aota et al J Biol Chem. 1994 Oct 7;269(40):24756-61

Insert

fibronectin (FN1 Human)

Use

Tags

Expression

Bacterial

Mutation

repeats 9 and 10; EcoR1 site on III-9 is mutated,…

Promoter

T7

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pFLAG CPTP

Plasmid

#170742

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

CPTP (CPTP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPm-DAD M1041A

Plasmid

#25411

Depositor

Arthur Alberts

Article

Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

Insert

Diap3 (Diaph3 Mouse)

Use

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Alanine substitution at critical residue in DAD t…

Promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67293

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Myt1L (Myt1l Mouse)

Use

Lentiviral

Tags

Expression

Mammalian and Yeast

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCsy3-VPR-Csy4

Plasmid

#153943

Purpose

Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Insert

Csy3-VPR, Csy4

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCsy1-Csy2

Plasmid

#153942

Purpose

Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Insert

Csy1, Csy2

Use

AAV and CRISPR

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Myc-gamma2s

Plasmid

#118036

Purpose

GABAA receptor expression (gamma2 short subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRG2 (Gabrg2 Rat)

Use

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR-PTPN11i1

Plasmid

#59612

Purpose

Expression of shRNA against human PTPN11

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPN11 (PTPN11 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPN11i2

Plasmid

#59613

Purpose

Expression of shRNA against human PTPN11

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPN11 (PTPN11 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pD765 HMGCR 3'UTR

Plasmid

#105766

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

HMGCR 3'UTR (HMGCR Human)

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-shAUF1 hairpin 1

Plasmid

#52921

Purpose

expresses a hairpin specific for human AUF1

Depositor

Sheila Stewart

Article

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting AUF1 (HNRNPD Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
Myc-delta

Plasmid

#118037

Purpose

GABAA receptor expression (delta subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRD (Gabrd Rat)

Use

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-shAUF1 hairpin 2

Plasmid

#52922

Purpose

expresses a hairpin specific for human AUF1

Depositor

Sheila Stewart

Article

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting AUF1 (HNRNPD Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T2 SHP2 D61A

Plasmid

#8323

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D61A (activated mutant)

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pET28a His6Flag2AUF1(p42)

Plasmid

#135941

Purpose

Expresses AUF1p42 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
UASgal-A9-Pleum

Plasmid

#60343

Purpose

Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LZRS ATG12 ires zeo

Plasmid

#67531

Purpose

For retroviral based stable expression of human ATG12

Depositor

Stephen Tait

Article

Haller et al Autophagy. 2014;10(12):2269-78. doi: 10.

Insert

APG12 (ATG12 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pET51b-His-TEV-SNAP-tag_cx

Plasmid

#167273

Purpose

Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Use

Tags

His x10

Expression

Bacterial

Mutation

P179R

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a His6Flag2AUF1(p40)

Plasmid

#135940

Purpose

Expresses AUF1p40 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK4-luciferase active (880)

Plasmid

#86657

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

867 base pairs including up to coding nucleotide …

Promoter

CDK5

Available since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-WRAP53beta-WD40domain

Plasmid

#64678

Purpose

Expresses the WD40 domain of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Wd40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

containing only WD40 domain of WRAP53beta

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pMyc-CMV1-huTLR4mut-C2141A

Plasmid

#53527

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C2141A (TLR4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

C2141A

Promoter

CMV

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
kank3_pJet1.2

Plasmid

#121981

Purpose

for generating kank3 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank3 (kank3 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_UG3

Plasmid

#133874

Purpose

Expresses UG3 in mammalian cells.

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH (UTRN Human)

Use

Tags

helical linker - mEGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CRYZL1_pCSdest

Plasmid

#53882

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CRYZL1 (CRYZL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pMyc-CMV1-huTLR4mut-C1196T

Plasmid

#53526

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C1196T (TLR4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

C1196T

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyc-CMV1-huTLR4mut-A896G

Plasmid

#53525

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 A896G (TLR4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

A896G

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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