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pDEST-CMV-3xFLAG-gateway-EGFP
Plasmid#122845PurposeN-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID
Plasmid#174213PurposeGFP::mAID donor backboneDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl
Plasmid#91248PurposeCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-Mxi1
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73022PurposeLuminescent and fluorescent dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
AbVec1.1-IGKC
Plasmid#80796PurposeExpression of immunoglobulin light chains in mammalian cells, human kappa constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlnRS-His
Plasmid#124108PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutaminyl-tRNA ligase (glnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.CAP-B10
Plasmid#175004Purposenon-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation systemDepositorInsertSynthetic construct isolate AAV.CAP-B10 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromoterelavl3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mPiezo1-IRES-eGFP
Plasmid#80925Purposeexpresses mouse Piezo1 with GFPDepositorInsertmouse Piezo1 CDS (Piezo1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRX-hSTING-EGFP
Plasmid#214149PurposeRetroviral transduction of EGFP-tagged STINGDepositorInsertSTING (STING1 Human)
UseRetroviralTagsEGFPExpressionMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLY100-RetroEFS-HER2-28BBz-WPRE
Plasmid#192201PurposeRetro-HER2CARDepositorInsertRetro-HER2CAR (ERBB2 Synthetic)
UseRetroviral; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-KRAB-blast
Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABExpressionMutationD10A and H840APromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 EGFPC2 TAZ
Plasmid#66850PurposeExpress GFP-fused TAZ by lentivirusDepositorInsertWWTR1/TAZ (WWTR1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-Malibu-mScarlet
Plasmid#229554PurposeFluorescent reporter for malonyl-CoADepositorInsertMalibu-mScarlet
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a-SerRS-His
Plasmid#124118PurposeBacterial expression plasmid for PURE system componentsDepositorInsertSeryl-tRNA ligase (serS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-PR rep/cap
Plasmid#197565Purposeencodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic deliveryDepositorInsertsAAV Rep genes
AAV9 VP1 Cap gene with PR insert
UseAAVTagsExpressionMutationcontains 7mer insert PRPPSTH between amino acids …PromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AlaRS
Plasmid#124103PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAlanyl-tRNA ligase (alaS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NF-kB-eCFP
Plasmid#118094PurposeFlourescent reporter for NF-kB activityDepositorInserteCFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NF-kB responsive elementAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG3K(1-10)
Plasmid#157993PurposeLentiviral vector of constitutive expression of mNeonGreen3K(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationG27D, D42E, M128I, K153M, T170IPromoterSFFVAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucOS
Plasmid#22777DepositorInsertOVA-SIY
UseCre/Lox and LentiviralTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoCjCas9-ABE8e-BlastR
Plasmid#194060PurposeMammalian expression of evoCjCas9 ABE8eDepositorInsertevoCjCas9-ABE8e
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone
Plasmid#216153PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic splice site and C-terminal FLAG tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-R-iLACCO1.2
Plasmid#208027PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1.2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AsnRS
Plasmid#124105PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAsparaginyl-tRNA ligase (asnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-His-ArgRS
Plasmid#124104PurposeBacterial expression plasmid for PURE system componentsDepositorInsertArgin+I4 ligase (argS Escherichia coli)
UseTags10x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6_B2M_sgRNA(PP7)
Plasmid#232438PurposesgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloopDepositorInsertPP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/F-tractin=mStayGold
Plasmid#212019PurposeFor filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertF-tractin
UseTagsmStayGoldExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_VPR_yl
Plasmid#107677PurposeCRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-VPR
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hChATp-tdTomato
Plasmid#197564PurposeExpresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragmentDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhuman ChAT promoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human V5-IRF3-pcDNA3
Plasmid#32713DepositorInsertIRF3 (IRF3 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-PheRS
Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorUseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR
Plasmid#112159PurposeGABA SensorDepositorHas ServiceAAV1InsertiGABASnFR
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF3195
Plasmid#144671PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertONECUT1 (ONECUT1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MetRS-His
Plasmid#124115PurposeBacterial expression plasmid for PURE system componentsDepositorInsertMethionine--tRNA ligase (metG Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AbVec1.1-IGLC2-XhoI
Plasmid#99575PurposeExpression of immunoglobulin light chains in mammalian cells, human lambda 2 constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE3
Plasmid#90337PurposeAHR - Aryl hydrocarbon receptor gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterAHRAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAspartate-tRNA ligase (aspS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
M51 Super 8x FOPFlash (TOPFlash mutant)
Plasmid#12457PurposeControl for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.DepositorInsertmutant TCF/LEF binding sites (Ctnnb1 )
UseLuciferaseTagsExpressionMammalianMutationmutant TCF/LEF binding sitesPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only
