170,188 results

Showing: 921 - 940 of 170188 results

Results per page:

Sort results by:

pCMV-SaPE2

Plasmid

#174817

Purpose

Mammalian expression of SaCas9 prime editor 2

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Plasmid

#163055

Purpose

Grx1-roGFP2 glutathione redox sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO/intron/GFP

Plasmid

#113547

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

GFP

Tags

GFP

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

Plasmid

#175573

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH_blast_MCS_Nard_GFP_LAMIN

Plasmid

#167340

Purpose

Expresses GFP fused to lamin A (LA) in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTomato-H2B-6

Plasmid

#58101

Purpose

Localization: Nucleus/Histones, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

H2B (H2BC11 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 BLTP3A

Plasmid

#241247

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) as a C-terminal fusion to EGFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) (BLTP3A Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pF1-Hs Cdk1/cyclin B1

Plasmid

#177330

Purpose

Co-expression of the human active Cdk1/cyclin B1 kinase complex

Depositor

Andreas Boland

Article

Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

Inserts

Cyclin-dependent kinase 1 (CDK1 Human)
G2/mitotic-specific cyclin-B1 (CCNB1 Human)

Tags

2 x Strep-tag and 8 x His

Expression

Insect

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
opto-RhoA-mCherry_pcDNA3.1

Plasmid

#164472

Purpose

Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

Insert

opto-RhoA

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2FLAG

Plasmid

#16331

Depositor

Peter Klein

Insert

Flag tag

Use

Vertebrate expression

Tags

Flag

Mutation

Flag tag inserted into pCS2+ polylinker

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-hOCT3/4-shp53-F

Plasmid

#27077

Purpose

Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

OCT3/4 (POU5F1 Human)

Expression

Mammalian

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GGamma8-GFP2

Plasmid

#140990

Purpose

Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma8-GFP2 (GNG8 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pG2K

Plasmid

#71742

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
cwby0005

Plasmid

#232214

Purpose

GGA cloning MS-his-AGGA-promoter-ccdb-TTCC

Depositor

David Baker

Article

Qian et al bioRxiv 2025.08.05.668824; doi: https://

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-ER D538G

Plasmid

#49500

Purpose

Expresses HA-tagged ER D538G mutant in mammalian cells

Depositor

Sarat Chandarlapaty

Article

Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Aspartic acid 538 to Glycine

Promoter

CMV

Available Since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only
pJAT31

Plasmid

#204291

Purpose

For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-DsRed

Use

CRISPR

Promoter

ie1

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-SREBP-2

Plasmid

#204200

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3

Expression

Mammalian

Mutation

silent mutations

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

Plasmid

#200400

Purpose

Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

Depositor

Daniel Kronauer

Article

Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

Inserts

Discosoma red fluorescent protein
Q factor 2
GCaMP6s

Expression

Insect

Promoter

15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge