163,606 results

1. pX458_Ruby

   Plasmid

   #110164

   Purpose

   Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

   Depositor

   Philip Hublitz

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and Ruby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EIF2AK2 gRNA (BRDN0001146342)

   Plasmid

   #75637

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMOD_C'0000a

   Plasmid

   #127226

   Purpose

   Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMOD_D0000a

   Plasmid

   #127230

   Purpose

   Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pegRNA-HEK3_del1-5-NGG

   Plasmid

   #185478

   Purpose

   pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

   Depositor

   Sabine Fuchs

   Article

   Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

   Insert

   HEK3_del1-5-NGG pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF0701

   Plasmid

   #143065

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PAXBP1 (PAXBP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-EF1a-MegaGate-DD-Hygro

   Plasmid

   #175267

   Purpose

   MegaGate destination with C-terminal DD tag and hygromycin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Tags

   Destabilization Domain

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMVV104

   Plasmid

   #215310

   Purpose

   Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PAraBAD

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX459-APP-KO

   Plasmid

   #176487

   Purpose

   To knockout APP gene

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIB170

    Plasmid

    #90193

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EIF2AK3 gRNA (BRDN0001145848)

    Plasmid

    #77166

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL50004

    Plasmid

    #50316

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mCherry variant of RFP (Discosoma sp.)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

    Plasmid

    #205281

    Purpose

    CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.

    Depositor

    Matthias Prigge

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKSJ155

    Plasmid

    #27126

    Depositor

    Alan Finkelstein

    Article

    Kienker et al J Gen Physiol. 2000 Oct . 116(4):587-98.

    Insert

    Colicin Ia + Ia immunity protein
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pJK225

    Plasmid

    #71124

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221_SLC16A9

    Plasmid

    #131876

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A9 (SLC16A9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pFA6a-hphMX6-Purg1-3FLAG

    Plasmid

    #19356

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    urg1 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

    Plasmid

    #70191

    Purpose

    Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    ClpX (clpX )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EIF2AK3 gRNA (BRDN0001146328)

    Plasmid

    #77167

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge