159,557 results

1. G10 Cas9 MDC123

   Plasmid

   #59187

   Purpose

   G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

   Depositor

   Robert Stupar

   Article

   Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

   Insert

   Glycine Max codon optimized Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   G10

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myc-βarrestin2-LOV-Turbo_pCDNA3

   Plasmid

   #199671

   Purpose

   expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Tags

   Myc and βarrestin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. T444T-GFP(NCO)

   Plasmid

   #183972

   Purpose

   Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

   Depositor

   David Matus

   Article

   Palmisano et al CourseSource, 2022

   Insert

   GFP (non-codon-optimized)
   

   Use

   RNAi

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

   Plasmid

   #69908

   Purpose

   Expresses a GFP circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-cfGFP

   Plasmid

   #200773

   Purpose

   Expresses cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   cysteine-free GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459V2.0-HypaCas9-mRuby2

   Plasmid

   #183872

   Purpose

   pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HypaCas9-P2A-mRuby2-T2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG.PKCzeta

   Plasmid

   #10799

   Depositor

   Alex Toker

   Article

   Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

   Insert

   PKC zeta (Prkcz Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

9. MSCV-Jarid1c-IRES-GFP

   Plasmid

   #166009

   Purpose

   Retroviral biscistronic expression of Jarid1c and eGFP

   Depositor

   Manu Rangachari

   Insert

   Jarid1c (Kdm5c Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-ON VIPER CAR (anti-CD19)-final design

    Plasmid

    #192117

    Purpose

    Lentiviral deliviery of ON VIPER CAR (anti-CD19)-final design

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid

    #216283

    Purpose

    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher003-TEVs-SpyTag003DA
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-PTK2

    Plasmid

    #23902

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAB988

    Plasmid

    #133920

    Purpose

    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

    Depositor

    Christopher Voigt

    Article

    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

    Insert

    miniICE-tet(M)cat(PC194)_PT7-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ub-R-YFP

    Plasmid

    #11948

    Depositor

    Nico Dantuma

    Article

    Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

    Insert

    Ub-R-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of YFP. Arginine at…

    Promoter

    Available since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac-GST-DAXX-His

    Plasmid

    #175781

    Purpose

    DAXX protein for baculovirus expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-mitoExRai-AKAR2

    Plasmid

    #161757

    Purpose

    ExRai-AKAR2 targeted to the outer mitochondrial membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    DAKAP-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

    Plasmid

    #166794

    Purpose

    Mammalian cell expression vector for Flag-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG.PKCzeta.K/W

    Plasmid

    #10800

    Depositor

    Alex Toker

    Article

    Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Kinase dead mutant: K281W (Lys 281 to Trp)

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pPD934 ZF37x6-Compact_EYFP

    Plasmid

    #138929

    Purpose

    EYFP reporter vector for the ZF37x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF37x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF37x6-Compact

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138128

    Purpose

    Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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