We narrowed to 14,583 results for: pET

1. pET-51b(+)-Caprola_01

   Plasmid

   #194662

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET3a aSyn murine

   Plasmid

   #108865

   Purpose

   Expresses murine alpha synuclein

   Depositor

   Gabriele Kaminski Schierle

   Insert

   alpha synuclein (Snca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETDUET-GST AO7REwt

   Plasmid

   #213935

   Purpose

   GST tagged AO7 encoding aa 126-258 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pet-15b-microID

   Plasmid

   #172881

   Purpose

   expresses microID in E. coli

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   microID
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-NLS-M-MLV-RT

   Plasmid

   #181804

   Purpose

   pET28a-NLS-M-MLV RT

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   NLS-M-MLV-RT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-51b(+)-cpHalo∆

   Plasmid

   #194659

   Purpose

   Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   cpHalo∆
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-Himar-dCas9

   Plasmid

   #137079

   Purpose

   Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BARD1 26-327 pET28a

   Plasmid

   #12646

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. UbcH5c S22R pET28a

   Plasmid

   #12644

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   Ubch5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

10. LHP2691 - pET30-Ub-K63A

    Plasmid

    #32179

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K63A

    Promoter

    T7

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. LHP2690 - pET30-Ub-K48A

    Plasmid

    #32178

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K48A

    Promoter

    T7

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pET28a SnoopTag-MBP

    Plasmid

    #72323

    Purpose

    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-MBP
    

    Use

    Tags

    N-terminal His6 tag. MBP is fused at the C-termin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-Streptavidin-Glutamate_Tag

    Plasmid

    #46367

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pET28-SpyCatcher002-MBP

    Plasmid

    #216295

    Purpose

    Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher002-MBP
    

    Use

    Tags

    His6 tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28C-mCherry-YTHDF2

    Plasmid

    #204406

    Purpose

    E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116954

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-pro-sumo1

    Plasmid

    #25101

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO1 (SUMO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Changed Serine 2 to Alanine

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pET28_human-pro-IL-18_DeltaN24

    Plasmid

    #214327

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Deletion of first 24 amino acids

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_Caspase-5-enzymatic-domain

    Plasmid

    #214306

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP5 (CASP5 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    aa133-434

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-BCA-Q11

    Plasmid

    #185781

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Q11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pet26-MBP-ELIC

    Plasmid

    #39239

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    Eriwinia chrysanthemi - pentameric ligand gated ion channel
    

    Use

    Tags

    His-10 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET30a-SARS-CoV2-NP

    Plasmid

    #165090

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pET28a-mH6-Cas12i1

    Plasmid

    #120882

    Purpose

    Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-Gate2 mazEF

    Plasmid

    #117117

    Purpose

    Destination vector with mazEF toxin antitoxin system

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Type

    Empty backbone

    Use

    Backbone for golden gate assembly for gene target…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET303-ColE1-T-E192C

    Plasmid

    #180233

    Purpose

    Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T domains (residue…

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28b-6His-AcpS

    Plasmid

    #145376

    Purpose

    Expression of E. coli AcpS

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    AcpS (acpP E. coli)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pET28a-SnoopTagJr-MBP

    Plasmid

    #105628

    Purpose

    Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SnoopTagJr-MBP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. wt HIV-1 integrase in pET15b

    Plasmid

    #61668

    Purpose

    wild type HIV-1 integrase in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    wild type HIV-1 integrase
    

    Use

    Tags

    His

    Expression

    Mutation

    Promoter

    T7 promotor

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pET-APOBEC1-YTHmut

    Plasmid

    #200159

    Purpose

    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHmut
    

    Use

    Tags

    6xHis, HA, and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET51b(+)-SNAP-CLIP

    Plasmid

    #127180

    Purpose

    Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

    Insert

    SNAP-CLIP
    

    Use

    Tags

    HisTag and StrepTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-Cre-6xHis

    Plasmid

    #62939

    Purpose

    Expression of Cre-6xHis in bacterial of cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cre recombinase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ScSpe1 pET-20b(+)

    Plasmid

    #117145

    Purpose

    Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    SPE1
    

    Use

    Tags

    Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET15b His6-ecDHFR (WT)

    Plasmid

    #73188

    Purpose

    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)

    Depositor

    Thomas Wandless

    Insert

    folA dihydrofolate reductase (folA E. coli)
    

    Use

    Tags

    His 6X

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pET28a T7pCONS sfGFP

    Plasmid

    #154462

    Purpose

    pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Bovine, Mouse, Rat, Human)
    

    Use

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 polymerase

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-SpyCatcher-mi3

    Plasmid

    #112255

    Purpose

    Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

    Insert

    SpyCatcher-mi3
    

    Use

    Tags

    C-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge