We narrowed to 22,146 results for: his

1. p2CT-His-MBP-Lba_Cas13a_WT

   Plasmid

   #91867

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbaCas13a (LbaC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-PLA2G15-6xHis S198A

   Plasmid

   #213605

   Purpose

   Expresses PLA2G15-6xHis S198A in mammalian cells

   Depositor

   Monther Abu-Remaileh

   Article

   Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

   Insert

   Phospholipase A2 Group XV (PLA2G15 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Changed serine 198 to alanine

   Promoter

   CMV

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-HIS-FLAG-AtUBQ11

   Plasmid

   #222720

   Purpose

   Expression HIS-FLAG-AtUBQ11 in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Insert

   AtUBQ11 (UBQ11 Mustard Weed)
   

   Use

   Tags

   2xFLAG and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACEBac1-PARP1-Flag-His-WT

   Plasmid

   #111572

   Purpose

   Expresses full length PARP1 in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/NDUFS3/myc-His

   Plasmid

   #127487

   Purpose

   Expresses myc-tagged NDUFS3 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS3 (NDUFS3 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-His6-Ran(Q69L)

   Plasmid

   #171563

   Purpose

   Encodes human Ran protein

   Depositor

   Yan Zeng

   Insert

   Ran (RAN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET32a-Trx-His6-TEV_SacB

   Plasmid

   #164588

   Purpose

   Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.

   Depositor

   Ernst Schonbrunn

   Insert

   TEV-SacB
   

   Use

   Tags

   Trx-His6 followed by TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTT3-SP-6xHis-KIR2DL5(FL)-FLAG

   Plasmid

   #157623

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL5 (KIR2DL5A Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET30-ARH3-His-Sumo-HA

   Plasmid

   #111578

   Purpose

   Expresses ARH3 (amino acids 14-363) in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   ARH3 (ADPRS Human)
   

   Use

   Tags

   6xHis-Sumo and HA

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-13

   Promoter

   T7

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b+His SUMO PPM1H

    Plasmid

    #207333

    Purpose

    Bacterial expression of His SUMO PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30a-His6-ABP-LOXCAT

    Plasmid

    #139971

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1-V5-HisB-ObKHC-wt-HaloTag

    Plasmid

    #201551

    Purpose

    Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

    Depositor

    Joshua Rosenthal

    Article

    Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

    Insert

    Kinesin-1 (partial)
    

    Use

    Tags

    HaloTag

    Expression

    Insect

    Mutation

    Codon-optimized for Drosophila expression

    Promoter

    Ac5

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/Myc-His(-)-MSulf-2

    Plasmid

    #13008

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    mouse sulfatase 2 (Sulf2 Mouse)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YEp195-CUP1-His-Ubi

    Plasmid

    #99540

    Purpose

    Yeast episomal vector for expression of His-tagged ubiquitin; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    UBI3(1-76) (RPL40A Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-GST-His6-1_HBD

    Plasmid

    #171869

    Purpose

    Expresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Hybrid binding domain of Rnase H1
    

    Use

    Tags

    GST- and His10-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-His10Smt3-gamma-toxin

    Plasmid

    #197000

    Purpose

    Bacterial expression plasmid for gamma toxin tRNA endonuclease

    Depositor

    Dragony Fu

    Article

    Lentini et al RNA. 2018 May;24(5):749-758. doi: 10.126

    Insert

    Gamma toxin
    

    Use

    Tags

    His10-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PET-B-evoSpCas9-NLS-6xHis

    Plasmid

    #207390

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PT7lacO-His6-PheRSa-PheRSb

    Plasmid

    #224364

    Purpose

    Express PheRS protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    PheRS (pheS E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFRT/TO/HIS/FLAG/HA-KIAA1967

    Plasmid

    #38082

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    KIAA1967 (CCAR2 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST TopBP1 (aa 32-1522) His

    Plasmid

    #20375

    Depositor

    Aziz Sancar

    Article

    Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Starts at aa32

    Promoter

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

21. pET-6xHis-(pos14)GFP

    Plasmid

    #89252

    Purpose

    Expresses GFP with +14 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-21a(+)/MBP-His

    Plasmid

    #38006

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    MBP (Maltose binding protein)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

23. His-ZZ-TEV-SNAPf-BICDR1

    Plasmid

    #111858

    Purpose

    N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Use

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    R250Q, Optimised for Sf9 expression

    Promoter

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA4-myc-His-mPGC-1a

    Plasmid

    #24925

    Depositor

    Toren Finkel

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

25. 6xHis-MBP-BrCas12b-FNLDTA

    Plasmid

    #195340

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis Tag and MBP, TEV cleavage site, 6x His Tag

    Expression

    Bacterial

    Mutation

    F208W, N524V, L795I, D868V, T874S, A1015E

    Promoter

    T7 Promoter

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5 BMPS C231S-6xHis

    Plasmid

    #204834

    Purpose

    Mammalian expression of recombinant BMPS C231S

    Depositor

    Monther Abu-Remaileh

    Article

    Medoh et al Science. 2023 Sep 15;381(6663):1182-1189

    Insert

    CLN5 (CLN5 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    C231S

    Promoter

    CMV

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. His6-TEV-Venus-pBluescript

    Plasmid

    #166756

    Purpose

    For purification of Venus fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Venus
    

    Use

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ET8-GPIba-high-6His

    Plasmid

    #180251

    Purpose

    High affinity mutant of human platelet membrane protein GP1b alpha

    Depositor

    Timothy Springer

    Article

    Jiang et al J Mol Biol. 2019 Mar 29;431(7):1380-1396

    Insert

    human GPIbalpha 1-290aa with mutation G233V and M239V (GP1BA Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    G233V and M239V

    Promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YEp181-CUP1-His-Ubi

    Plasmid

    #99539

    Purpose

    Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    UBI3(1-76) (RPL40A Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His6-TEV-mCerulean3-pBluescript

    Plasmid

    #166757

    Purpose

    For purification of mCerulean3 fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3
    

    Use

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET20b-NLuc-MBP-6xHis

    Plasmid

    #135931

    Purpose

    Expresses NanoLuciferase in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pES002 MBP-GSK3β_S9A-HA-His

    Plasmid

    #196184

    Purpose

    Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    HA, His6, and MBP

    Expression

    Bacterial

    Mutation

    changed Serine 9 to Alanine

    Promoter

    Tac

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS423-CUP1-His-Ubi

    Plasmid

    #99541

    Purpose

    Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    UBI3(1-76) (RPL40A Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-6xHis-(pos15)GFP

    Plasmid

    #89248

    Purpose

    [Edit] Expresses GFP with +15 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos15)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBAD/HisB (TIREVOL)-sfGFP

    Plasmid

    #189675

    Purpose

    titratable expression of sfGFP in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    sfGFP
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

    Plasmid

    #190928

    Purpose

    Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Tags

    Strep-TEV-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pcold-6xHis-HRV3Csite-HsTRBP

    Plasmid

    #41096

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    6xHis-HRV3Csite

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pFA6a-link-yoEGFP-SpHis5

    Plasmid

    #44836

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-HCMVgH-8his (Merlin)

    Plasmid

    #136447

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-716). Codon-optimize…

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge