165,307 results

1. pTK-EdnrbE1-Citrine

   Plasmid

   #127773

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE1
   

   Use

   Enhancer reporter construct

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PiggyBac PlayBack

   Plasmid

   #203312

   Purpose

   To all for PiggyBac gene insertion into Genome

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-puro

   Plasmid

   #39481

   Purpose

   Tetracycline inducible 3rd generation lentiviral vector

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5 and 6XHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXR001: EF1a-CasRx-2A-EGFP

   Plasmid

   #109049

   Purpose

   CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Insert

   CasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   2A-eGFP, HA, and NLS

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-miniTurbo-NLS-Blast

   Plasmid

   #232347

   Purpose

   Lentiviral control vector expressing miniTurboNLS without dCas9

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   miniTurbo
   

   Use

   Lentiviral

   Tags

   3xHA and 3xSV40 NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCM-AAVS1-TO-hNGN2

   Plasmid

   #105840

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-T7pol

   Plasmid

   #59926

   Purpose

   Expression vector for T7 RNA polymerase gene

   Depositor

   Ian Wickersham

   Insert

   T7 RNA polymerase
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE6a

   Plasmid

   #207851

   Purpose

   Mammalian expression of PE6a prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6a
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. human beta-catenin pcDNA3

   Plasmid

   #16828

   Purpose

   Mammalian expression of human beta-catenin with Flag tag

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV2/1

    Plasmid

    #112862

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap1
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDx_mScarlet3

    Plasmid

    #189754

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. tFucci(CA)5

    Plasmid

    #153521

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(CA)5.
    

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG.LSL.tdTomato

    Plasmid

    #100048

    Purpose

    AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdtomato
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Promoter

    pCMV-EGFP

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid

    #58993

    Purpose

    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

    Depositor

    Snorri Thorgeirsson

    Article

    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

    Inserts

    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    EcoRV site in MCS converted to BamHI site

    Promoter

    CMV enhancer-chicken beta actin

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Expression

    Bacterial

    Promoter

    native promoter

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

    Plasmid

    #234826

    Purpose

    STITCHR R2Tocc editor

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    nCas9h840a-xten-R2Tocc
    

    Use

    CRISPR

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HA

    Plasmid

    #128034

    Purpose

    Mammalian expression vector with N terminal HA tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMV306G13+Lux

    Plasmid

    #26160

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    G13 promoter + Bacterial luciferase operon
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Expression

    Plant

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. LentiV_Cas9_Blast

    Plasmid

    #125592

    Purpose

    Lentiviral expression of spCas9 with blasticidin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSF3-ultraID

    Plasmid

    #172878

    Purpose

    expresses ultraID in mammalian cells with the tet-on or tet-off system

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Use

    Luciferase and Retroviral; To be used in a tet-on…

    Tags

    myc

    Expression

    Mammalian

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    TRE-cmv

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. eGFP-ova-C1

    Plasmid

    #163524

    Purpose

    Expresses both eGFP and chicken ovalbumin in mammalian cells

    Depositor

    Thomas Weber

    Insert

    enhanced-GFP-chicken ovalbumin tandem
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEcgRNA

    Plasmid

    #166581

    Purpose

    CRISPR-Cas9-assisted genome editing in Escherichia coli

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    ccdB
    

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. v5 ABE-eVLP

    Plasmid

    #228467

    Purpose

    Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–ABE8e
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. kiCAP-AAV-BI30

    Plasmid

    #183749

    Purpose

    AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
    

    Use

    AAV

    Mutation

    K449R, and NNSTRGG inserted between 588 and 589 o…

    Promoter

    p41

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid

    #174660

    Purpose

    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

    Depositor

    Beverly L. Davidson

    Article

    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

    Insert

    miniXon cassette
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. lentiMPH v2

    Plasmid

    #89308

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Insert

    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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