161,242 results

1. CASR-Tango

   Plasmid

   #66235

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CASR (CASR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLAMP1-mCherry

   Plasmid

   #45147

   Depositor

   Amy Palmer

   Article

   Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

   Insert

   LAMP1-mCherry (LAMP1 Synthetic, Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1_3XFlag

   Plasmid

   #182494

   Purpose

   Empty plasmid containing Flag tag for C-terminal tagging

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

   Insert

   3xFlag with 5 Glycolic spacer
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-LSSmGFP

   Plasmid

   #186543

   Purpose

   LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB_r5-HT1.0

   Plasmid

   #208713

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiV2-EF1α-nCas9/RT

   Plasmid

   #210188

   Purpose

   Expression of nCas9/RT-P2A-Puro

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   nCas9/RT-P2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Epac-S-H188

   Plasmid

   #170349

   Purpose

   mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgGria1

   Plasmid

   #124853

   Purpose

   Mutagenesis of Gria1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria1 (Gria1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX311 MCL1

   Plasmid

   #117729

   Purpose

   Open reading frame vector encoding MCL1

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   MCL1L (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLOX-TERT-iresTK

    Plasmid

    #12245

    Purpose

    2nd generation lentiviral vector for reversible immortalization of human primary cells

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    TERT, HSV1-TK (TERT Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiV2-mU6-evopreQ1

    Plasmid

    #210190

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CL20_mEGFP_BCR_ABL1

    Plasmid

    #205780

    Purpose

    Express mEGFP-tagged fusion protein, BCR_ABL1_p210 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    BCR_ABL1 (BCR, ABL1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDiGi

    Plasmid

    #59323

    Purpose

    Inducible expression of dsRed and GFP in bacteria

    Depositor

    David Holden, Sophie Helaine

    Article

    Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

    Inserts

    EGFP
    dsRed
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and Plac

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CD74-ROS1 G2032R

    Plasmid

    #183814

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Retroviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    G2032R

    Promoter

    CMV

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-flag-GFP-NCAM-1

    Plasmid

    #205190

    Purpose

    This vector encodes of a synCAM (NCAM1) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM NCAM1 and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti.CMV/TO_PRKAA1

    Plasmid

    #74446

    Purpose

    Lentiviral expression vector encoding untagged WT human AMPK alpha 1

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-MS2-HA-HshnRNPC1_Z

    Plasmid

    #148114

    Purpose

    Mammalian Expression of HshnRNPC1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPC1 (HNRNPC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDB_345

    Plasmid

    #231113

    Purpose

    ABE activity-based selection cloning vector

    Depositor

    David Root, John Doench

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs_FLAG-NMNAT1

    Plasmid

    #133259

    Purpose

    Express FLAG-tagged NMNAT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT1 (NMNAT1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-22b DT 51E/148K

    Plasmid

    #11081

    Depositor

    John Collier

    Insert

    DT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    51E/148K, catalytically inactive

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-BRD4

    Plasmid

    #65378

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    R1232W

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHDE-35S-Cas9-mCherry-UBQ

    Plasmid

    #78932

    Purpose

    For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

    Depositor

    Yunde Zhao

    Article

    Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CDK9_pLX307

    Plasmid

    #98324

    Purpose

    Lentiviral expression of CDK9

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    CDK9 (CDK9 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. Halo-VSVG-RUSH

    Plasmid

    #166907

    Purpose

    RUSH cargo: Str-Ii_IRES_VSVG-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-C3-ZO-2

    Plasmid

    #27422

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

27. nCas9-PPE

    Plasmid

    #140445

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    nCas9(H840A)-M-MLV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A for Cas9; D200N, T306K, W313F, T330P and L…

    Promoter

    maize Ubiquitin-1

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CABE-T3.155

    Plasmid

    #193270

    Purpose

    Expression of CABE-T3.155 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T3.155
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TRE-mCherry-p2A-Chrimson-TS

    Plasmid

    #158702

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    TRE-mcherry-p2A-Chrimson-TS
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. THINGL pcDNA3.1

    Plasmid

    #230046

    Purpose

    Expressing the human version of TINGL biosensor for glucose (named THINGL) in mammalian cells

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    THINGL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge