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We narrowed to 18,759 results for: nls

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Showing: 2081 - 2120 of 18759 results

  2. pGGD_RT_li_Tq2CFP_2NLS

    Plasmid
    #136972
    Purpose
    Goldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Expression
    Bacterial
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136972-plasmid-map-sequence-id-269108

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  4. ABE9

    Plasmid
    #194208
    Purpose
    Mammalian expression of ABE9
    Depositor
    Dali Li
    Publication
    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-8e (N108Q/L145T)-nSpCas9
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194208-plasmid-map-sequence-id-473532

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  5. PX458-AAVS1

    Plasmid
    #113194
    Purpose
    Encodes gRNA for human AAVS1
    Depositor
    Adam Karpf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 (PPP1R12C, AAVS1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113194-plasmid-map-sequence-id-216314

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  6. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Publication
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59896-plasmid-map-sequence-id-313658

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  10. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nm-dCas9-p300 Core (EP300 Human, Neisseria meningiditis, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61365-plasmid-map-sequence-id-244292

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  12. pCMV-PE2-P2A-hMLH1dn

    Plasmid
    #174827
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-hMLH1dn
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174827-plasmid-map-sequence-id-343635

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  13. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Publication
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162074-plasmid-map-sequence-id-471574

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  17. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135955-plasmid-map-sequence-id-269512

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  19. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62888-plasmid-map-sequence-id-105911

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  21. pLJM1-Halo-NLS

    Plasmid
    #250858
    Purpose
    lentiviral vector for expressing HaloTag protein with an SV40 NLS signal
    Depositor
    Britt Glaunsinger
    Insert
    HaloTag
    Use
    Lentiviral
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    250858-plasmid-map-sequence-id-498584

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  27. pBT281

    Plasmid
    #122611
    Purpose
    express evoAPOBEC1-BE4max in mammalian cells
    Depositor
    David Liu
    Publication
    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    evoAPOBEC1-BE4max
    Expression
    Mammalian
    Mutation
    E4K H109N H122L D124N R154H A165S P201S F205S
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122611-plasmid-map-sequence-id-238891

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  33. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Publication
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85452-plasmid-map-sequence-id-173969

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  36. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid
    #114071
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R and S542R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114071-plasmid-map-sequence-id-220509

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  37. mRuby-PCNA-19-NLS-4

    Plasmid
    #55876
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 NLS (PCNA Human)
    Tags
    3xNLS and mRuby
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 2081 - 2120 of 18759 results