Addgene

We narrowed to 13,476 results for: cas9 genes

Showing: 2651 - 2700 of 13476 results

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pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

Plasmid

#236242

Purpose

Lentiviral expression of SpCas9

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SpCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

Plasmid

#223123

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9=D10A

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

Plasmid

#223102

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)

Plasmid

#223103

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MRKQKC)-P2A-EGFP (RAS2838)

Plasmid

#223104

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRKQKC)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MRKQKC)=D1135M, S1136R, G1218K, E1219Q, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MRKSKC)-P2A-EGFP (RAS2840)

Plasmid

#223105

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRKSKC)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MRKSKC)=D1135M, S1136R, G1218K, E1219S, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

Plasmid

#223106

Purpose

pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-mTagBFP2

Expression

Mammalian

Mutation

SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

Plasmid

#223107

Purpose

pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-mTagBFP2

Expression

Mammalian

Mutation

SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

Plasmid

#223108

Purpose

pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-mTagBFP2

Expression

Mammalian

Mutation

SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

Plasmid

#223109

Purpose

pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-mTagBFP2

Expression

Mammalian

Mutation

SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(KWKYES)-P2A-EGFP (RAS2900)

Plasmid

#223095

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWKYES amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KWKYES)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(KWKYES)=D1135K, S1136W, G1218K, E1219Y, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(LWKYEY)-P2A-EGFP (RAS3001)

Plasmid

#223096

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYEY amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(LWKYEY)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(LWKYEY)=D1135L, S1136W, G1218K, E1219Y, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MKACKV)-P2A-EGFP (AHK136)

Plasmid

#223097

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKACKV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MKACKV)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MKACKV)=D1135M, S1136K, G1218A, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MKRCKS)-P2A-EGFP (AHK140)

Plasmid

#223098

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MKRCKS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MKRCKS)=D1135M, S1136K, G1218R, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(KRRCKV)-P2A-EGFP (RAS3014)

Plasmid

#223099

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KRRCKV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KRRCKV)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(KRRCKV)=D1135K, S1136R, G1218R, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(VKMAKG)-P2A-EGFP (RAS3015)

Plasmid

#223100

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with VKMAKG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(VKMAKG)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(VKMAKG)=D1135V, S1136K, G1218M, E1219A, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(SKNCKS)-P2A-EGFP (RAS2951)

Plasmid

#223101

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SKNCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(SKNCKS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(SKNCKS)=D1135S, S1136K, G1218N, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

Plasmid

#223087

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(KWAVIG)-P2A-EGFP (RAS2999)

Plasmid

#223088

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWAVIG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KWAVIG)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(KWAVIG)=D1135K, S1136W, G1218A, E1219V, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MWRQLM)-P2A-EGFP (RAS2868)

Plasmid

#223089

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWRQLM amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MWRQLM)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MWRQLM)=D1135M, S1136W, G1218R, E1219Q, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(KWHMLM)-P2A-EGFP (RAS2869)

Plasmid

#223090

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWHMLM amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KWHMLM)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(KWHMLM)=D1135K, S1136W, G1218H, E1219M, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MRKCLN)-P2A-EGFP (AHK130)

Plasmid

#223091

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCLN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRKCLN)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MRKCLN)=D1135M, S1136R, G1218K, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(LWKFEG)-P2A-EGFP (RTW3931)

Plasmid

#223092

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKFEG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(LWKFEG)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(LWKFEG)=D1135L, S1136W, G1218K, E1219F, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(CWNWNS)-P2A-EGFP (RAS3011)

Plasmid

#223093

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with CWNWNS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(CWNWNS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(CWNWNS)=D1135C, S1136W, G1218N, E1219W, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(LWRYEK)-P2A-EGFP (RAS2986)

Plasmid

#223094

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(LWRYEK)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(LWRYEK)=D1135L, S1136W, G1218R, E1219Y, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(LWKYSS)-P2A-EGFP (RAS3024)

Plasmid

#223080

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYSS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(LWKYSS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(LWKYSS)=D1135L, S1136W, G1218K, E1219Y, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MWKYQA)-P2A-EGFP (RAS2965)

Plasmid

#223081

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWKYQA amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MWKYQA)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MWKYQA)=D1135M, S1136W, G1218K, E1219Y, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(LWQYQH)-P2A-EGFP (RAS2885)

Plasmid

#223082

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWQYQH amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(LWQYQH)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(LWQYQH)=D1135L, S1136W, G1218Q, E1219Y, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(RQMYQG)-P2A-EGFP (RAS3009)

Plasmid

#223083

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RQMYQG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(RQMYQG)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(RQMYQG)=D1135R, S1136Q, G1218M, E1219Y, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MWKYQS)-P2A-EGFP (RAS2898)

Plasmid

#223084

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MWKYQS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MWKYQS)=D1135M, S1136W, G1218K, E1219Y, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(LWSSLY)-P2A-EGFP (RAS2997)

Plasmid

#223085

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWSSLY amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(LWSSLY)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(LWSSLY)=D1135L, S1136W, G1218S, E1219S, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(KWMQLC)-P2A-EGFP (RAS3025)

Plasmid

#223086

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWMQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KWMQLC)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(KWMQLC)=D1135K, S1136W, G1218M, E1219Q, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(RRTIMR)-P2A-EGFP (AHK149)

Plasmid

#223071

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RRTIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(RRTIMR)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(RRTIMR)=D1135R, S1136R, G1218T, E1219I, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MRRCQR)-P2A-EGFP (RAS2823)

Plasmid

#223072

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRCQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRRCQR)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MRRCQR)=D1135M, S1136R, G1218R, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(IRAVQL)-P2A-EGFP (RTW3820)

Plasmid

#223073

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with IRAVQL amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(IRAVQL)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(IRAVQL)=D1135I, S1136R, G1218A, E1219V, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(SRSCMV)-P2A-EGFP (RTW3732)

Plasmid

#223074

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRSCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(SRSCMV)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(SRSCMV)=D1135S, S1136R, G1218S, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MKRCMV)-P2A-EGFP (AHK162)

Plasmid

#223075

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MKRCMV)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MKRCMV)=D1135M, S1136K, G1218R, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MWNVML)-P2A-EGFP (AHK156)

Plasmid

#223076

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWNVML amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MWNVML)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MWNVML)=D1135M, S1136W, G1218N, E1219V, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MKKCMN)-P2A-EGFP (AHK129)

Plasmid

#223077

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MKKCMN)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MKKCMN)=D1135M, S1136K, G1218K, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(KKKCMN)-P2A-EGFP (AHK131)

Plasmid

#223078

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KKKCMN)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(KKKCMN)=D1135K, S1136K, G1218K, E1219C, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(MRRWMR)-P2A-EGFP (RAS2816)

Plasmid

#223068

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRRWMR)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(ARGIMR)-P2A-EGFP (AHK147)

Plasmid

#223069

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(ARGIMR)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(ARGIMR)=D1135A, S1136R, G1218G, E1219I, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(ARTWGR)-P2A-EGFP (RAS2995)

Plasmid

#223070

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARTWGR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(ARTWGR)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(ARTWGR)=D1135A, S1136R, G1218T, E1219W, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

Plasmid

#228251

Purpose

pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP

Use

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

Plasmid

#234883

Purpose

non-targeting CRISPRi control

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

L1HS gRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#226834

Purpose

Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Olactb and U6

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

Plasmid

#226989

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 1a to create OPA1-del_exon5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

Plasmid

#226991

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2a to create OPA1-del_exon5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)

Plasmid

#226987

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A6 to create OPA1-R194G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

Plasmid

#226986

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A7 to create OPA1-R194G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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