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We narrowed to 2,817 results for: ALC

Showing: 2701 - 2750 of 2817 results

  3. pCAG-mEGFP-CaMKIIa (T305D/T306D)

    Plasmid
    #127611
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)
    Depositor
    Ryohei Yasuda
    Article
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 305/306 to Alanine
    Promoter
    pCAG
    Available since
    Jan. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    127611-plasmid-map-sequence-id-250748

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  4. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid
    #100021
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100021-plasmid-map-sequence-id-194294

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  13. pUC19-K2FTY8

    Plasmid
    #103079
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    K2FTY8(Cas9 coding gene from Alcanivorax pacificus W11-5)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103079-plasmid-map-sequence-id-201719

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  18. GFP Cav2.2 W391A pRK5

    Plasmid
    #206084
    Purpose
    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding
    Depositor
    Annette Dolphin
    Article
    Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    W391A
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    206084-plasmid-map-sequence-id-412224

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  20. P2061

    Plasmid
    #186299
    Purpose
    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10
    Inserts
    LexA-HBD-B112
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    2xLexop-minimalCyc1 and PACT1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186299-plasmid-map-sequence-id-367190

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  21. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

    Plasmid
    #123354
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    cp-moxCerulean3-moxCerulean3-FLARE-D1ER
    Use
    Tags
    C-terminal ER retention signal (KDEL), cp-moxCeru…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    123354-plasmid-map-sequence-id-311156

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  27. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Javier García-Sancho, Teresa Alonso
    Article
    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9
    Insert
    erGAP3
    Use
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    78118-plasmid-map-sequence-id-153361

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  28. pcDNA3-Venus-cpVenus-FLARE-Cameleon

    Plasmid
    #123350
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-Cameleon
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123350-plasmid-map-sequence-id-307103

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  30. PB-CAG-ChR2-YFP

    Plasmid
    #204345
    Purpose
    PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.
    Depositor
    Ivo Lieberam
    Article
    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi
    Insert
    ChR2-YFP
    Use
    Tags
    Yellow fluorescent protein
    Expression
    Mammalian
    Mutation
    C-terminus (aa315-737) is deleted - PMID: 1461559…
    Promoter
    CAG
    Available since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    204345-plasmid-map-sequence-id-410725

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  31. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid
    #127392
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)
    Depositor
    Ryohei Yasuda
    Article
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Aspartic acid and Threon…
    Promoter
    pCAG
    Available since
    July 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    127392-plasmid-map-sequence-id-250433

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  33. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid
    #49332
    Purpose
    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Article
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Insert
    CACNA1D (Cacna1d Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…
    Promoter
    CMV
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    49332-plasmid-map-sequence-id-215242

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  37. pSYC-437

    Plasmid
    #163829
    Purpose
    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.
    Depositor
    Seok-Yong Choi
    Article
    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:
    Inserts
    Foxj1a Promoter
    GCaMP6s
    Use
    Multisite gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163829-plasmid-map-sequence-id-323196

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  39. CaMKIV-DCTK75E

    Plasmid
    #126423
    Purpose
    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Article
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Insert
    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed lysine 75 to glutamic acid
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126423-plasmid-map-sequence-id-249663

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  46. pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500

    Plasmid
    #92229
    Purpose
    Expression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    NTerm 6xHis Tagged zmADH
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70b (p70a promoter with one mutation)
    Available since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92229-plasmid-map-sequence-id-181872

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  49. Ultra-Exon1Q145-Myc-A

    Plasmid
    #110487
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110487-plasmid-map-sequence-id-212608

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Showing: 2701 - 2750 of 2817 results