4,168 results

1. pCMVR8.74

   Plasmid

   #22036

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Insert

   gag pol tat rev
   

   Expression

   Mammalian

   Available Since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-jGCaMP8f

   Plasmid

   #162373

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-HA-USP33

   Plasmid

   #22601

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP33 (USP33 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-RelA

   Plasmid

   #23255

   Depositor

   Warner Greene

   Article

   Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

   Insert

   p65 (RELA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-HA-USP16

   Plasmid

   #22595

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP16 (USP16 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Dec. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ152

   Plasmid

   #69303

   Purpose

   Gateway entry vector with pco-dCas9-VP64

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-VP64 (Plant codon-optimized)
   

   Use

   CRISPR

   Mutation

   VP64 activator fusion; NLS fusion

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AbVec1.1-IGKC

   Plasmid

   #80796

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-XBP1 mNeonGreen NLS

   Plasmid

   #115968

   Purpose

   ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   X-box binding protein 1 (XBP1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGH8 - pRAB-3::mCherry::unc-54utr

   Plasmid

   #19359

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Prab-3::mCherry
   

   Tags

   mCherry

   Expression

   Worm

   Available Since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-TIGHT-EGFP-donor fw copy

    Plasmid

    #22074

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    TETO-eGFP
    

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZipGFP-Casp3

    Plasmid

    #81241

    Purpose

    A ZipGFP-based Caspase-3 protease reporter T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

    Insert

    ZipGFP with caspase cleavage sequence T2A mCherry
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid

    #50475

    Purpose

    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-mito-R-GECO1

    Plasmid

    #46021

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18R6KT-mini-Tn7T-Km

    Plasmid

    #64969

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pTP1122

    Plasmid

    #104159

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. N-Cadherin-EGFP

    Plasmid

    #18870

    Depositor

    Valeri Vasioukhin

    Article

    Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

    Insert

    N-Cadherin (Cdh2 Mouse)
    

    Tags

    EGFP, Kanamycin, and N-Cadherin

    Expression

    Mammalian

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. mKeima-Red-Mito-7

    Plasmid

    #56018

    Purpose

    Localization: Mitochondria, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Insert

    Mito (COX8A Human)
    

    Tags

    mKeima-Red

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-CD36-C-10

    Plasmid

    #55011

    Purpose

    Localization: Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD36 (CD36 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-HA-JMJD3

    Plasmid

    #24167

    Purpose

    Mammalian expression of human JMJD3 with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    JMJD3 (KDM6B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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