4,202 results

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Ubc.Luc.IRES.Puro

Plasmid

#33307

Depositor

Linzhao Cheng

Article

Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pALB-GFP

Plasmid

#55759

Purpose

Fluorescent marker for hepatocytic lineage

Depositor

Snorri Thorgeirsson

Article

Heo et al Hepatology. 2006 Dec;44(6):1478-86.

Insert

albumin enhancer/promoter (Alb Mouse)

Expression

Mammalian

Promoter

Mouse Albumin Enhancer/Promoter

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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HA-Halotag-FRB-EX-nes_pLX304

Plasmid

#120913

Purpose

Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

HA-Halotag-FRB-EX-NES

Use

Lentiviral

Expression

Mammalian

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP33

Plasmid

#22601

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP33 (USP33 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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Klotho (secreted)

Plasmid

#17713

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

His and V5

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8HOIP

Plasmid

#50015

Purpose

Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

ring finger protein 31 (RNF31 Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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moxCerulean3

Plasmid

#68066

Purpose

C-terminal fusion to moxCerulean3

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

moxCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pBS aP2 promoter (5.4kb) polyA

Plasmid

#11424

Depositor

Ronald Kahn

Insert

aP2 promoter (Fabp4 Mouse)

Expression

Bacterial

Available Since

April 3, 2006

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-c-Met

Plasmid

#31784

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pDESTmycYBX1

Plasmid

#19878

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Y box binding protein 1 (YBX1 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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Myosin-IIA-GFP

Plasmid

#38297

Depositor

Matthew Krummel

Article

Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

Insert

Myosin II A (Myh9 Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mCherry FP

Plasmid

#52114

Purpose

a bicistronic IRES-mCherry containing retroviral vector

Depositor

Dario Vignali

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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AbVec1.1-IGKC

Plasmid

#80796

Purpose

Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-2

Plasmid

#26807

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)

Tags

2x FLAG

Expression

Mammalian

Mutation

L7V, G76S, K395R and I482L

Promoter

CMV

Available Since

July 26, 2011

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

Plasmid

#128604

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH1

Plasmid

#108699

Purpose

For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH1 (nuclear isoform) (RNASEH1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGRB

Plasmid

#71539

Purpose

for expression of gRNA in E. coli

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Type

Empty backbone

Expression

Bacterial

Promoter

J231196

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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