-
Plasmid#39996DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_105_Venus_173
Plasmid#40001DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_159_Venus_173
Plasmid#40006DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_227_Venus_229
Plasmid#40018DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_175_Venus_229
Plasmid#40013DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNB691
Plasmid#60135PurposeRed beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB753
Plasmid#60148PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterLEU1prAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_105_Venus_157
Plasmid#40000DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_175_Venus_wt
Plasmid#39828DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_227_Venus_wt
Plasmid#39833DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_159_Venus_wt
Plasmid#39823DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE95 mFZ6-CS2+
Plasmid#16802DepositorInsertmFZ6-CS2+ (Fzd6 Mouse)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-10u
Plasmid#13550DepositorInsertFLIP-E intramol A207R (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207R, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
XE105 XPKC alpha-myc-CS2+
Plasmid#16745DepositorInsertXPKC-alpha-myc (prkca Frog)
UseXenopus expressionTagsc-mycExpressionMutationPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Grx1-RoTq-On
Plasmid#234467PurposeMammalian expression of Grx1-RoTq-OnDepositorInsertGrx1-RoTq-On
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pG0A0-1-1-PBAD-dCas9
Plasmid#208815PurposeExpresses arabinose-inducible dCas9, codon-optimized for SalmonellaDepositorInsertArabinose-inducible dCas9 that is codon optimized for expression in Salmonella
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterArabinsoe-inducible promoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-His-E1GFP
Plasmid#233885PurposeBacterial Expression of recombinant His-tagged E1GFPDepositorInsertE1GFP
UseTagsHisExpressionBacterialMutationT65S and T203Y compared to EGFPPromoterT7 promoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPorig
Plasmid#221092PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-NFAPorig
Plasmid#221091PurposeFAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-NFAPorig
Plasmid#221086PurposeFAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-CFAPorig
Plasmid#221087PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDIV302
Plasmid#204973PurposeAMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance markerDepositorInsertsMad7
nat1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc0---MS2
Plasmid#210572PurposeExpresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc100---MS2
Plasmid#210573PurposeExpresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-optimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc0-malat1
Plasmid#210580PurposeExpresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc100-malat1
Plasmid#210581PurposeExpresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-UGCG-EGFP
Plasmid#225878PurposePlasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertionDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-ATP
Plasmid#225247PurposeBacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-glucose
Plasmid#225252PurposeBacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucoseDepositorInsertqmTQ2-glucose
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-cAMP
Plasmid#225249PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
A99-ELP
Plasmid#170760PurposeA99 laminin α1 peptide fused to I30-ELP (VPGIG)30DepositorInsertA99-ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA-GFP
Plasmid#220189PurposesgRNA expression vector - pEM7 promoter with GFP in sgRNA siteDepositorInsertpEM7 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyB-GFP
Plasmid#220190PurposesgRNA expression vector - pBG35 promoter with GFP in sgRNA siteDepositorInsertpBG35 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD-GFP
Plasmid#220192PurposesgRNA expression vector - pBG28 promoter with GFP in sgRNA siteDepositorInsertpBG28 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-GFP
Plasmid#220197Purposemini-Tn7 GFP expression vectorDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMJ074
Plasmid#215654PurposepET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143DepositorInsertYellow fluorescent protein
UseSynthetic BiologyTags10x histidine tagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-ORP5deltaTMD-FKBP
Plasmid#220073Purposeexpresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.DepositorInsertOSBPL5deltaTMD (OSBPL5 Human)
UseTagsExpressionMammalianMutationdeltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-DR
Plasmid#216266PurposeDox-responsive module fused with EGFPDepositorInsertCodon-optimized TetR - Lipid binding motif (KRf - KLK) - Codon-optimized TetR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4693
Plasmid#200784PurposePtwk-40s EGFP unc-54 3' UTR C.elegans AVA and other neurons expression of EGFPDepositorInsertno
UseTagsGFPExpressionWormMutationPromoterPtwk-40sAvailable sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4506
Plasmid#200255PurposePnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFPDepositorInsertTeTx
UseTagsmCherryExpressionWormMutationPromoterPnpr-4Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH1371
Plasmid#199631PurposePrgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFPDepositorInsertGPI
UseTagsYFPExpressionWormMutationPromoterPregf-1Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
pYSD063
Plasmid#212916PurposeEncodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertmRuby2
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-mCh-bla
Plasmid#210260PurposeIntegrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
mCherry
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA11-pMa-sgTS3-17xMS2V5-sgTS3
Plasmid#199442PurposeCRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology armsDepositorInsertHITI donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-ASAP3ER
Plasmid#195358PurposeASAP3ER under control of human synapsin promoterDepositorInsertASAP3ER
UseAAVTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-eDHFR
Plasmid#194635PurposeMammalian expression of the eDHFR control bait in VirotrapDepositorInsertp55 GAG
UseTagsecDHFRExpressionMammalianMutationPromoterSRalphaAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_SulR(CDS)
Plasmid#186423Purposeprovides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)DepositorInsertSulR
UseSynthetic Biology; Cds (part b3-b4-b5), goldenbra…TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only