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Plasmid#74694PurposeSrpR-S4 gateDepositorInsertPTac-RBSS4-SrpR-PSrpR-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBM3R1-B3
Plasmid#74680PurposeBM3R1-B3 gateDepositorInsertPTac-RBSB3-BM3R1-PBM3R1-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBM3R1-B1
Plasmid#74678PurposeBM3R1-B1 gateDepositorInsertPTac-RBSB1-BM3R1-PBM3R1-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
UseTagsExpressionBacterialMutationN/APromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pML3009
Plasmid#193256PurposeGamma-aminobutyric acid sensor expressing eYFP reporterDepositorInsertsgabR
eYFP
UseSynthetic BiologyTagsExpressionMutationPromotersynthetic promoter P2 and synthetic promoter PGab…Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pKS1
Plasmid#24636DepositorInsert'tesA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDF-EcAvs2
Plasmid#188804PurposeCodon optimized EcAvs2 with C-terminal His tagDepositorInsertEcAvs2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB239
Plasmid#170034PurposeEncodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD009
Plasmid#116850PurposeExpresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009DepositorInsertsAraC
alpha-HL
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS011
Plasmid#65464PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherryDepositorInsertMBP-Ntag-mCherry
UseTagsFLAG and MBPExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL5
Plasmid#72989PurposeRBS_M2DepositorInsertM2 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL11
Plasmid#72995PurposeRBS_L4DepositorInsertL4 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-lacIZ
Plasmid#158061PurposeDonor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosomeDepositorInsertlacIZ operon
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120033127
Plasmid#46011DepositorInsertECK120033127 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-170nΔ13V
Plasmid#27999DepositorInsertFLIPglu-170nΔ13V
UseTagseCFP and eYFPExpressionYeastMutationWT binding protein (no F16A)PromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pHelper_O
Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationE59G - please see depositor commentsPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_W
Plasmid#173784PurposeThe Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.DepositorInsertWphi Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_D
Plasmid#173781PurposeThe D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.DepositorInsertD145 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TDGS50
Plasmid#183189PurposeTEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoterDepositorInsertsDivIVA-GFP(tevR M0050)
TEV-S219V
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGK415-rtetTAK8N, L131L
Plasmid#165969PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastMutationPromoterpPGK1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-170nDelta13
Plasmid#13562DepositorInsertFLIPglu F16F Delta13
UseTagsECFP and EYFPExpressionBacterialMutationWT binding protein (no F16A)PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120015170
Plasmid#46006DepositorInsertECK120015170 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL4
Plasmid#72988PurposeRBS_H5DepositorInsertH5 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL8
Plasmid#72992PurposeRBS_L1DepositorInsertL1 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L1U1H09
Plasmid#73008PurposeL1U1H09 terminatorDepositorInsertL1U1H09 terminator
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p15aC-4D1D
Plasmid#107864PurposeBacterial expression of PIS and PI4KDepositorInsertsPIS
phosphatidylinositol 4-kinase β
UseTagsmycExpressionBacterialMutationPromoterproDAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJAT13
Plasmid#18986DepositorInsertpromoter CP13
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSEL2
Plasmid#23012DepositorInsertKanamycin resistance
UseTags6 His Tag and Xpress epitopeExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSELt_S7.A
Plasmid#125835PurposeNegative selection plasmid for evolution of an amber stop codon suppression tRNADepositorInsertACP-2xAmb-pheS
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpr.trpL*Available sinceMay 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZH509
Plasmid#102664PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-YedK
Plasmid#84567PurposeExpresses YedK in mammalian cellsDepositorInsertYedK
UseTags3xFlag and C-MycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEL1324
Plasmid#139874PurposeExpression plasmid for WT LetB (without TM region) for cryo and negative stain EMDepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877)-6xHisPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120010783
Plasmid#46009DepositorInsertECK120010783 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pDUET-ctCPR-nAMO
Plasmid#20110DepositorInsertp450 reductase and native Amorphadiene oxidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK125108723
Plasmid#46008DepositorInsertECK125108723 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH848-gst-disec
Plasmid#89589PurposePlasmid for bacterial expression of His-tagged GST encoded by non-preferred codonsDepositorArticleInsertglutathione-S transferase codon-dis-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD010
Plasmid#116851PurposeExpresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010DepositorInsertsAraC
alpha-HL
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS012
Plasmid#65465PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherryDepositorInsertNtag-mCherry
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF-EcAvs4
Plasmid#188806PurposeCodon optimized EcAvs4 with C-terminal His tagDepositorInsertEcAvs4
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSaI14
Plasmid#8614DepositorInsertRibonuclease inhibitor SaI14
UseTagsExpressionBacterialMutationN terminal threonine changed to alaninePromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120010800
Plasmid#46010DepositorInsertECK120010800 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKB234.2
Plasmid#170023PurposeEncodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
hBD3 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceDec. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZH512
Plasmid#102666PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDUET-ctCPR-BovAMO
Plasmid#20113DepositorInsertp450 reductase, and amorphadiene oxidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pES E38A
Plasmid#13049DepositorInsertMutS
UseTagsHis6ExpressionBacterialMutationmutation Glu38 to AlaPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGC4780
Plasmid#113408PurposeTranslation reporter with inducible translation coupling (high native GFP expression)DepositorInsert158_33121123_1
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only