We narrowed to 18,352 results for: Tec

1. EasyFusion T2A-H2B-TagBFP

   Plasmid

   #113086

   Purpose

   General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-TagBFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DL017:CMV::HaloTag-iPAK4

   Plasmid

   #177882

   Purpose

   HT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BFP dest clone

   Plasmid

   #71825

   Purpose

   Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mEmerald-Keratin-17

   Plasmid

   #54134

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. EasyFusion T2A-H2B-mCherry

   Plasmid

   #113089

   Purpose

   General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBZS-M.CviPI

   Plasmid

   #198355

   Purpose

   Express M.CviPI in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviPI (M.CviPI )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pR26-CAG-H2B-miRFP703

   Plasmid

   #112853

   Purpose

   Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   H2B-miRFP703
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB2191

   Plasmid

   #67538

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET29b_1_anti-GFP-nanobody

   Plasmid

   #210394

   Purpose

   Express anti-GFP nanobody in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   1_anti-GFP-nanobody
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. 6xHis-MBP-huBsCas12a

    Plasmid

    #174671

    Purpose

    Expresses 6xHis-MBP tagged BsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 TaGu N-1F

    Plasmid

    #203762

    Purpose

    For expression of N-flag tagged TaGu R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBT100.164

    Plasmid

    #138522

    Purpose

    phage cloning

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(7.10)-inteinN
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p8.9 NdeltaSB D185K/D186L

    Plasmid

    #132931

    Purpose

    p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequence

    Depositor

    Jeremy Luban

    Article

    De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

    Insert

    gag and pol D185K/D186L
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D185K/D186L

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV_AncBE4max_3xHA

    Plasmid

    #112097

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max_3xHA
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCIG3 B

    Plasmid

    #132942

    Purpose

    MLV packaging plasmid for B-tropic MLV gag-pol expression

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    B-tropic MLV gag-pol
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD-HisD-miniGFP2

    Plasmid

    #188897

    Purpose

    Express 6xHis-Tag miniGFP2 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCHERRY3

    Plasmid

    #24659

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DL033:cFos::eGFP-iPAK4

    Plasmid

    #177883

    Purpose

    eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cFos

    Available since

    July 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ttAtCas12a+int

    Plasmid

    #182384

    Purpose

    Gives very high editing efficiency in barley. Also works in Brassica oleracea

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al bioRxiv 2022.04.28.489853

    Insert

    LbCas12a coding sequence with D156R and Arabidopsis introns
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R + 8 Arabidopsis introns added

    Promoter

    None

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYL322d1/N-eGFP

    Plasmid

    #183151

    Purpose

    Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMR-A-3

    Plasmid

    #138517

    Purpose

    P3a

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRC0569 (parts entry vector)

    Plasmid

    #189966

    Purpose

    Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BBa-J23100

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT2ST

    Plasmid

    #59384

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-dfrA
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pD441_WT_hi

    Plasmid

    #128857

    Purpose

    High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG1344 EGFP-FIREtag

    Plasmid

    #205165

    Purpose

    Expresses EGFP-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    EGFP-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQ265_OoA3GX2

    Plasmid

    #225147

    Purpose

    Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

    Insert

    OoA3GX2-zCas9(D10A)-UGI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 6xHis-MBP-huTsCas12a

    Plasmid

    #174687

    Purpose

    Expresses 6xHis-MBP tagged TsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huTsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC19/N-Vc

    Plasmid

    #183157

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pscAAV-CBh-aSyn-WPRE3-enSV40pA

    Plasmid

    #194244

    Purpose

    Expresses human alpha-Synuclein WT

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    aSynuclein(WT) (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pLKO.1P shSOD2

    Plasmid

    #102976

    Purpose

    Suppression of SOD2

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shSOD2 (SOD2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMCB306

    Plasmid

    #89360

    Purpose

    sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 6xHis-MBP-huBoCas12a

    Plasmid

    #174673

    Purpose

    Expresses 6xHis-MBP tagged BoCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBoCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. IVT-MCP-Phi29

    Plasmid

    #207456

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    MCP-Phi29
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAGT10446

    Plasmid

    #192353

    Purpose

    L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-t35S_CGCT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28c-Ku

    Plasmid

    #161936

    Purpose

    Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

    Depositor

    Harris Wang

    Article

    Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

    Insert

    ku (mku Mycobacterium tuberculosis)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 6xHis-MBP-huPb2Cas12a

    Plasmid

    #174686

    Purpose

    Expresses 6xHis-MBP tagged Pb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-VCre

    Plasmid

    #89575

    Purpose

    Expresses VCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    VCre recombinase
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA-RNF4/pRK5

    Plasmid

    #59743

    Purpose

    Expresses RNF4 in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET30-T7-His-sfGFP

    Plasmid

    #180755

    Purpose

    pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCellFree_G03 TFEC

    Plasmid

    #67060

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFEC (TFEC Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

42. pASTA3

    Plasmid

    #24657

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. MSGV Hu Acceptor

    Plasmid

    #64269

    Purpose

    Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. NM1-5S-tRNA-SgH

    Plasmid

    #128178

    Purpose

    Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    5S rRNA-tRNA(Arg)

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET21a.Tat(48-57)-EGFP

    Plasmid

    #165040

    Purpose

    Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    EGFP
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGNW2

    Plasmid

    #122086

    Purpose

    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCMVTnT-Flp-3xFLAG

    Plasmid

    #109167

    Purpose

    Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a mammalian expression system

    Depositor

    Monika Golas

    Article

    Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

    Insert

    Flp
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. p201B Cas9

    Plasmid

    #59177

    Purpose

    Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    BAR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. 4xNLS-pMJ915v2-sfGFP

    Plasmid

    #88921

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge