163,606 results

1. pFA6a-link-yoEGFP-CaURA3

   Plasmid

   #44872

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-OSF-PP

   Plasmid

   #154247

   Purpose

   backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Type

   Empty backbone

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-GH

   Plasmid

   #60514

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and hygromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAd5-B1-deltaE1-GFP

   Plasmid

   #179203

   Purpose

   Block 1 with deletion in E1 and insertion of GFP expression cassette

   Depositor

   Fred Bunz

   Article

   Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

   Insert

   Modified Block 1 with deletion in E1 and insertion of GFP expression cassette
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mito-PAGFP

   Plasmid

   #23348

   Purpose

   Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

   Depositor

   Richard Youle

   Article

   Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

   Insert

   PAGFP
   

   Use

   Tags

   mito

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Clover-Prx2-mRuby2

   Plasmid

   #117906

   Purpose

   fluorescent reporter of cytosolic hydrogen peroxide

   Depositor

   Hadley Sikes

   Article

   Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

   Insert

   Clover-Prx2-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX307 P2A GFP Luciferase

   Plasmid

   #193674

   Purpose

   Constitutive lentiviral expression of Luciferase

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GRAB-rDA2h

   Plasmid

   #208701

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA2h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. JC Mad-1

   Plasmid

   #25626

   Depositor

   Peter Howley

   Article

   Howley et al J Virol. 1980 Dec . 36(3):878-82.

   Insert

   JC Virus Mad-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

10. SL016: AAV-CAG-FLEX-CheRiff-GFP

    Plasmid

    #124776

    Purpose

    For AAV based expression of CheRiff in Cre-Positive cells

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    CheRiff-GFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

11. pMito-TrxRFP1

    Plasmid

    #98997

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and mitochondrial localization sequence
    

    Use

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOT_1 - lenti-EFS-LTBR-2A-puro

    Plasmid

    #181970

    Purpose

    Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    LTBR (LTBR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid

    #107292

    Purpose

    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-GFP (SNCA Human)
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDRF1-GW yoeGFP-T2A-mCherry

    Plasmid

    #118446

    Purpose

    Produces equimolar expression of yeast codon-optimized eGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yoeGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-EF1adCas9VP64T2ABsd-W

    Plasmid

    #112919

    Purpose

    Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9VP64-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. SRPK1 (SRPK1A-c100)

    Plasmid

    #98246

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SRPK1A (SRPK1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PlepA

    Plasmid

    #55175

    Purpose

    Strong constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    lepA promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. UnReg-H2B-YFP-DD

    Plasmid

    #96892

    Purpose

    Reporter to monitor translation of unstructured mRNA

    Depositor

    Tobias Meyer

    Article

    Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

    Insert

    UnReg-H2B-Venus-DD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET11a+CYP119

    Plasmid

    #66131

    Purpose

    Expresses the wild type of CYP119

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

    Plasmid

    #128164

    Purpose

    UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
    

    Use

    Synthetic Biology

    Tags

    FLAG and zif268

    Expression

    Bacterial

    Mutation

    Promoter

    UV5

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFETCh_RAD21

    Plasmid

    #64056

    Purpose

    Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RAD21 Homology Arms (RAD21 Human)
    

    Use

    Tags

    3X Flag P2A Neo

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid

    #128163

    Purpose

    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    ERBIN PDZ domain (ERBIN Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG and Omega subunit of RNA polymerase

    Expression

    Bacterial

    Mutation

    Promoter

    UV5

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDONR221-SPNS1_STOP

    Plasmid

    #161439

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SPNS1 (SPNS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. FUW-RLuc-T2A-PuroR

    Plasmid

    #102320

    Purpose

    expression of r-luciferase

    Depositor

    Rudolf Jaenisch

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ubc

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAG-MCS-EGFP

    Plasmid

    #164092

    Purpose

    expression of EGFP-tagged proteins under CAG promoter

    Depositor

    Maik Hintze

    Insert

    EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-YFP-KRAS4B

    Plasmid

    #112718

    Purpose

    Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    YFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-OSF-PP-human-CHMP3

    Plasmid

    #154180

    Purpose

    expresses human CHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (CHMP3 Human)
    

    Use

    Tags

    FLAG and Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid

    #183765

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    ChR2-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    sgRNA resistant

    Promoter

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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