We narrowed to 10,106 results for: UTY
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Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
TagsHisExpressionBacterialAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL004
Plasmid#98920PurposeConstitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.DepositorInsertGCaMP6f tethered to mRuby
TagsHis and mRuby3ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT371
Plasmid#125612PurposeExpresses CDA1-BE4max-NG in mammalian cellsDepositorInsertCDA1-BE4max-NG
ExpressionMammalianAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K63C
Plasmid#86589PurposeBacterial expression of Ubiquitin K63C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
TagsHis6-MBPExpressionBacterialMutationTEV site; K63CPromoterTacAvailable SinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB-CAG-Tfe3-pgk-hph
Plasmid#48755PurposePiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selectionDepositorAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTREX GPR68_NB2_2
Plasmid#127691PurposePlasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.DepositorInsertGPR68_NB2_2
ExpressionMammalianAvailable SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 5HT2A_NB2_1
Plasmid#127690PurposePlasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.DepositorInsert5HT2A_NB2
ExpressionMammalianAvailable SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRRH
Plasmid#136924PurposeExpresses Cas9-NRRH in mammalian cellsDepositorInsertCas9-NRRH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015
Plasmid#202023PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
ExpressionBacterialMutationGvpA T6A-I48VAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-DN-14-3-3 zeta (R56A/R60A)
Plasmid#116889PurposeExpresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cellsDepositorAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMVV108
Plasmid#215338PurposeExpresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPSalTTCAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG
Plasmid#139961PurposeOver-expression of heterologus squalene epoxidase from Nannochloropsis oceanicaDepositorInsertConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
UseSynthetic BiologyAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0377
Plasmid#115509PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFPDepositorInsertsfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-v5.9
Plasmid#155259PurposePlasmid for the aTc-inducible expression of the v5.9DepositorInsertv5.9
Tags6xHisExpressionBacterialPromotertet PAAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIN REP5-GFP-GluR2 (Q)
Plasmid#24003DepositorAvailable SinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-mF(3230)A3
Plasmid#168016PurposeExpresses MBP-F(3230)A3-6xHis in bacterial cellsDepositorInsertMBP-F(3230)A3-6xHis
TagsHisx6 and maltose binding proteinExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJWV25
Plasmid#61045PurposeTo construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaADepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMN-285
Plasmid#195472PurposeLentiviral Integratable Inducible CD19CAR using synZiFTR ZF10DepositorInsert8X ZF10 BS - ybTATA - CD19 CAR - mCherry
UseLentiviralExpressionMammalianAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPANI2A2E2-Crimson
Plasmid#193758PurposeExpresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertE2-Crimson
UseSynthetic BiologyExpressionBacterialPromoterPANI2 synthetic promoter carrying binding sites f…Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
mOtop3_pGEMHE
Plasmid#114676PurposeThis construct was used to express mouse Otop3 in Xenopus laevis oocytesDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG223 PRKD1 3'UTR mut
Plasmid#12042DepositorInsertPRKD1 3'UTR mut (PRKD1 Human)
UseLuciferaseExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…Available SinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFW5000
Plasmid#224214PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb dPspCas13b + PspCas13b guide RNA
Plasmid#176306PurposeExpresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
TagsHA tagExpressionInsectMutationH133A, H1058APromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMVV207
Plasmid#215343PurposegRNA expression for S. stutzeri nifL deletion on an RSF1010 backboneDepositorInsertS. stutzeri nifL gRNA
UseCRISPR and Synthetic BiologyTagsNoneExpressionBacterialPromoterJ23119Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Histone H2B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45216DepositorInsertHistone H2B-targeted Aurora B FRET sensor
ExpressionMammalianPromoterT7Available SinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pCMB-TMEr
Plasmid#61208PurposeFluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoterDepositorInsertMtBCPsp-mCherry-BCP
TagsmCherryExpressionPlantPromoterMtBCP1Available SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS_235
Plasmid#195460PurposeLentiviral Integratable, 4OHT/TMX-inducible expression of synZiFTR: ZF3-p65DepositorInsertpSFFV - ZF3 - p65 - ERT2
UseLentiviralExpressionMammalianAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT44c
Plasmid#138511PurposeP2DepositorInsertinteinC-dSpCas9-UGI
UseSynthetic BiologyAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2H12
Plasmid#221142PurposecdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)DepositorInsertcdGreen
ExpressionBacterialPromoterPtetAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa
Plasmid#78182PurposeExpresses ffDronpa E. coli strainsDepositorInsertffDronpa
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
TagsHisExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneExpressionBacterial and InsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL-Vinculin_TurboID_Venus
Plasmid#200212PurposeLentivirus vector expressing vinculin with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculin_TurboID_Venus
UseLentiviralPromoterT7Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA M66I
Plasmid#149687PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).DepositorInsertCapsid
UseLentiviral and RetroviralExpressionMammalianMutationChanged Capsid residue Met 66 to Iso (CA M66I)Available SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
mOtop2_pGEMHE
Plasmid#114675PurposeThis construct was used to express mouse Otop2 in Xenopus laevis oocytesDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTNSQCas
Plasmid#219434PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInsertTnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
ExpressionBacterialAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-LoxP
Plasmid#219436PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInserttwo transposon ends, LoxP-terminator-LoxP gene cassette
ExpressionBacterialAvailable SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNDGG004
Plasmid#231317PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Tc-par-ELT4; GG assembly from pOGG011, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG051
Plasmid#231320PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG053
Plasmid#231322PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT6-G60A-FLAG
Plasmid#102323PurposeHuman SIRT6 G60A with FLAG tag for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
TagsFLAGExpressionMammalianMutationchanged glycine 60 to alaninePromoterCMVAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOtop2_pcDNA3
Plasmid#114678PurposeThis construct was used to express mouse Otop2 in HEK-293 cellsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4
Plasmid#135460PurposeMammalian expression of uFLARE TF componentDepositorInsertCMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
TagsHA, flag,ExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
ExpressionBacterialPromoterE . coli insulated waaH gene promoter, low phosp…Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Vinculin_TurboID_Venus
Plasmid#200272PurposeExpressing vinculin with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculin_TurboID_Venus
ExpressionMammalianPromoterT7Available SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
BbZIP-cALFA
Plasmid#160498PurposeFor the protein expression of BbZIP with ALFA tag at C-terminus in E. coliDepositorInsertBbZIP
TagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only