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PB-TRE-dCas9-VPR

Plasmid

#63800

Purpose

SP-dCas9-VPR with doxycycline-inducible expression

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-LC3

Plasmid

#11546

Depositor

Karla Kirkegaard

Article

Jackson et al PLoS Biol. 2005 May . 3(5):e156.

Insert

LC3B (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2CreERT2

Plasmid

#13777

Purpose

Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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c-Jun

Plasmid

#187902

Purpose

Expresses c-Jun in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

c-Jun (JUN Human)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 transition and G2/M transition, one of two plasmids for the FUCCI4 system

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD24-sfGFPx1

Plasmid

#51558

Purpose

Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

Depositor

Sankar Adhya, Francisco Malagon

Article

Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

Insert

superfolder GFP

Expression

Bacterial

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSF1389

Plasmid

#104962

Purpose

Bacterial expression plasmid of bdSENP1 protease

Depositor

Dirk Görlich

Article

Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

Insert

SENP1

Tags

His14 tag and Tev protease cleavage site

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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ubbr:GCaMP8m

Plasmid

#232485

Purpose

To make fish transgenic lines that have calcium sensor expression in the whole body of the fish

Depositor

Chie Satou

Article

Ruetten et al bioRxiv [Preprint]. 2025 Sep 20:2025.08.

Insert

jGCaMP8m

Use

Zebrafish expression

Promoter

ubbr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact-tdTomato

Plasmid

#64048

Purpose

Fluorescent reporter for F-actin labeling in living cells

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Lifeact

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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GST-RBD

Plasmid

#15247

Depositor

Martin Schwartz

Article

Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

Insert

Rhotekin (Rtkn Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 7-89 (Rho binding domain)

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG

Plasmid

#91797

Purpose

Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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peYFP-C1-mTOR

Plasmid

#73384

Purpose

Expresses YFP tagged mTOR

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mTOR (MTOR Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGFPGUSPlus

Plasmid

#64401

Purpose

Binary plant vector expressing GFP and GUS, both driven by a 35S promoter

Depositor

Claudia Vickers

Article

Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

Inserts

GFP
GUSPlus

Use

Binary vector for plant transformation

Tags

His

Expression

Plant

Mutation

S65T

Promoter

35S

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pZT-C13-R1

Plasmid

#62197

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Right CLYBL TALEN

Use

TALEN

Promoter

CMV

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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FUGW-PercevalHR

Plasmid

#49083

Purpose

Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE8e-NRCH

Plasmid

#165416

Purpose

Expresses the base editor ABE8e-NRCH in mammalian cells

Depositor

David Liu

Insert

ABE8e-NRCH

Tags

BP-NLS

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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Nuclear PancACe

Plasmid

#215706

Purpose

Expresses acetyl-CoA biosensor in the nucleus

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO1-shYAP1

Plasmid

#27368

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

shYAP1

Expression

Mammalian

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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Nuclear cpGFP

Plasmid

#215707

Purpose

Expresses cpGFP in the nucleus

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NORAD

Plasmid

#120383

Purpose

Expression vector for lncRNA NORAD

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

NORAD (NORAD Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti:FlipGFP-T2A-mCherry

Plasmid

#223198

Purpose

Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

Depositor

Yeh-Hsing Lao

Article

Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

Insert

FlipGFP-T2A-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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lentiMPH v2

Plasmid

#89308

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Insert

MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF0

Plasmid

#200383

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTNS2

Plasmid

#64968

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

tnsABCD

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-VSVG

Plasmid

#11912

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F64L, S65T, A206K relative the wild type GFP sequ…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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