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We narrowed to 59,686 results for: plasmid

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Showing: 1261 - 1280 of 59686 results

  6. pHelper_ShCAST_sgRNA

    Plasmid
    #127921
    Purpose
    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).
    Depositor
    Feng Zhang
    Publication
    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127921-plasmid-map-sequence-id-333562

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  9. pDAC439

    Plasmid
    #195237
    Purpose
    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Publication
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    Expression
    Mammalian
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195237-plasmid-map-sequence-id-382945

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  12. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71287-plasmid-map-sequence-id-352460

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Showing: 1261 - 1280 of 59686 results