We narrowed to 57,324 results for: plasmid
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Plasmid#91907PurposeBackbone for cloning LwCas13a guides under tRNAval promoterDepositorInsertLwCas13a Guide site
ExpressionMammalianAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-SARS-Spike
Plasmid#145031PurposeExpress SARS-CoV spike protein with C9 tag at C-terminal in mammalian cellsDepositorInsertSARS-CoV Spike (S Synthetic)
TagsC9 and CD5 signal peptideExpressionMammalianPromoterCMVAvailable sinceApril 16, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pGEMHE-vhhGFP4-hIgG1-Fc
Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas serviceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE
Plasmid#180280PurposeThe CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.DepositorInsertCasMINI sgRNA (backbone) and BFP
UseLentiviralExpressionMammalianPromoterU6Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAnc80L65AAP
Plasmid#92307PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAPDepositorInsertAncestral AAV Capsid Anc80L65AAP
UseAAV and Synthetic BiologyExpressionMammalianPromoterRep2Available sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianPromotertet-onAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nb-gp41-Halo (MoonTag-Nb-Halo)
Plasmid#128603PurposeExpresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and HaloPromoterSFFVAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TA-ERP2
Plasmid#80477PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-jGCaMP8m-WPRE
Plasmid#162375PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas serviceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-Cas9-2A-GFP-sgRNA
Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPPromoterMSCVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tetO.Nfib.Hygro
Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianPromotertet-onAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pdCas9-DNMT3A-EGFP (ANV)
Plasmid#71685PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSynap.iGABASnFR
Plasmid#112159PurposeGABA SensorDepositorHas serviceAAV1InsertiGABASnFR
UseAAVPromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA
Plasmid#190163PurposeMicroglia-targeted transgene expression.DepositorInsertsmIba1 promoter, 1.7-kb, microglia-specific promoter
miR-9-Tx4, 4 repeated miRNA target sites for miR-9
miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
UseAAVAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-FLEX-TeLC-P2A-dTomato
Plasmid#159102PurposeCre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.DepositorInsertTeLC-P2A-NLS-dTomato
UseAAVPromoterhSynapsinAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by