We narrowed to 5,559 results for: chia
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Plasmid#175804PurposeComplementation of yebST knockout in E. coli K-12DepositorInsertyebST
UseTagsNoneExpressionBacterialMutationsilent mutations to introduce restriction sitesPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast
Plasmid#213117PurposeExpression of a faster-running MinE mutant in mammalian cellsDepositorInsertMinE(L4E)-EGFP (minE Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationL4EPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-FRB-mCherry-MinD-IRES-Puro
Plasmid#213116PurposeExpression of FRB-mCherry-MinD in mammalian cells for payload deliveryDepositorInsertFRB-mCherry-MinD (minD Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1DA
Plasmid#35028DepositorInsertFEN1DA (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationD181A--NUCLEASE DEAD POINT MUTANTPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)
Plasmid#110554PurposeminiTn7 delivery plasmid with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoterAraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
Plasmid#171129PurposeLwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)DepositorInsertU6 promoter entry plasmid for LwaCas13a crRNA cloning
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA
Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluSnFR
Plasmid#106123PurposeIndicator for glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR
UseTagsMyc and PDGFRExpressionMammalianMutationPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1deltaP
Plasmid#35030DepositorInsertFEN1DELTAP (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutation8 AMINO ACID DELETION OF P BOX (amino acids 337–3…PromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135T
Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
UseTagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLicC-MBP-APETx2
Plasmid#72668PurposePeriplasmic expression of APETx2 in E.coliDepositorInsertAPETx2
UseTagsHis tag, MBP, MalE signal sequence for periplasmi…ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMALcPP-MS2cp
Plasmid#203351PurposeExpresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteriaDepositorInsertMS2 coat protein
UseTagsHx6-MBPExpressionBacterialMutationV75E and A81G, to prevent capsid assemblyPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAL_dstβL
Plasmid#73805PurposePlasmid for selection for insertion and homodimerizationDepositorInsertsCAT Marker
ToxR
beta lactamase
spectomycin resistance
CLS TM domain
UseTagsPart Of the dstbl chiemra, Part of the dsTbL chim…ExpressionBacterialMutationPromoterNaN and ToxR promotorAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a WT-ER
Plasmid#8355DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationmouse estrogen receptor (G525R) on C-termPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSNK
Plasmid#135193PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoteraraBADAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal NLS
Plasmid#188396PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptide and SV40 NLSExpressionBacterialMutationPromoterPhyper spankAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-AtC60αβC20NtL
Plasmid#160876PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a WT delta CT
Plasmid#1796DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationdelta CT (aa468-673)PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHL 1075
Plasmid#53004PurposepCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsfhlA
sodB
UseSynthetic BiologyTagsGFP and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a WT DBM (H212R)
Plasmid#8352DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationH212RPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136458
Plasmid#149531PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSF-pMB1'-t7- YFP
Plasmid#186564PurposepMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro
Plasmid#213118PurposeExpression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cellsDepositorInsertPKAsub-mCherry-MinD (minD Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTag-P2A-QFGal4-SV40pA
Plasmid#155127PurposeDonor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld methodDepositorInsertP2A-QFGal4
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgNeg1
Plasmid#166998PurposeLentiviral expression of negative control sgRNA for CRISPRi knockdownDepositorInsertNegative control sgRNA 1
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB cdc42 WT
Plasmid#61635Purposemammalian expression of cdc42 wtDepositorInsertcdc42 (CDC42 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRCP
Plasmid#195862PurposeEncodes chromogenic protein (CP). CP inducible with rhamnose.DepositorInsertChromogenic Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1079A
Plasmid#83494PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)DepositorInsertLbu_C2c2_R1079A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1079APromoterT7Available sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSNA
Plasmid#135192PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoteraraBADAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGK:TRL-EFS:iRFP670
Plasmid#68475PurposeLentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670DepositorInsertstTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFS and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2NtB2
Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateTagsExpressionMutationThe 3’-end of the chloramphenicol resistance gene…PromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
p168
Plasmid#62618PurposeePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046799
Plasmid#145577PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only