12,913 results

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pcDNA3.1-HA-mSTING (V154M)

Plasmid

#214159

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (V154M) (Sting1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

N153S

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS

Plasmid

#221313

Purpose

Expression of MAVS in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pT2-shATRX-GFP

Plasmid

#124259

Purpose

Expresses shRNA targeting ATRX with a GFP reporter which is driven by the SV40 promoter. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Expression

Mammalian

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

Plasmid

#182232

Purpose

Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member A (RHOA Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Promoter

TRE bidirectional

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHA-mLRH-1

Plasmid

#16342

Depositor

Austin Cooney

Article

Gu et al Mol Cell Biol. 2005 May . 25(9):3492-505

Insert

LRH-1 (Nr5a2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Rfx4

Plasmid

#61544

Purpose

Expresses Rfx4 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Rfx4 (Rfx4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pRN3P-HA-Suv4-20h1

Plasmid

#86689

Purpose

For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)

Tags

HA-tag

Promoter

T3

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-VGlut2 [N29/29R]

Plasmid

#114501

Purpose

Mammalian Expression Plasmid of anti-VGlut2 (Rat). Derived from hybridoma N29/29.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-VGlut2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a6 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-KLC2-N-18

Plasmid

#62747

Purpose

Localization: Kinesin Light Chain , N1 Cloning Vector, Excitation:587, Emission:610

Depositor

Michael Davidson

Insert

KLC2

Tags

mCherry

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only
pIRESh-GFPII-MB-Dlk1

Plasmid

#108930

Purpose

GFP-tagged vector expressing membrane-bound Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Tags

GFPII

Expression

Mammalian

Mutation

lacks coding sequence for the juxtamembrane motif…

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mouse cdk6 wt

Plasmid

#75170

Purpose

Mammalian expression of mouse cdk6

Depositor

Martine Roussel

Insert

cyclin dependent kinase 6 (Cdk6 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pLKO-CreERT2 sgAdam10 v3

Plasmid

#158047

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-puro-Flag-mMcl-1

Plasmid

#32982

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
FRIG-myc-Sema4D-CD4

Plasmid

#51606

Purpose

expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Use

Lentiviral

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

RSV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein N-ter

Plasmid

#73520

Purpose

Encodes the N-terminus of mouse ninein (residues 1-373) with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein N-ter (residues 1-373) (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pcDNAI Neo mouse PECAM FL

Plasmid

#42901

Depositor

Steven Albelda

Insert

PECAM (Pecam1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA-HA-LATS2-KR

Plasmid

#33100

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

lats2 (Lats2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

K655R, Kinase Dead

Promoter

CMV

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
pD2529-CAG-Flag-mouse TGFb1

Plasmid

#180260

Purpose

Mouse full length TGFβ1 expression

Depositor

Timothy Springer

Article

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

TGFβ1 (Tgfb1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-nArgBP2

Plasmid

#74514

Purpose

Express GFP-nArgBP2 fusion protein in mammalian cells

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2 (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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Ptch1-mCherry

Plasmid

#118982

Purpose

Expresses mCherry tagged Ptch1 in mammalian cells

Depositor

Paul Hamel

Insert

Ptch1 (Ptch1 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

see depositor comments below

Promoter

CMV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il6

Plasmid

#207126

Purpose

Luciferase vector containing 3'UTR for mouse Il6

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 6 (Il6) (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GluA2 [L21/32R]

Plasmid

#177480

Purpose

Mammalian Expression Plasmid of anti-GluA2/GluR2 glutamate receptor (Rat). Derived from hybridoma L21/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluA2/GluR2 glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Gria2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Smyd2

Plasmid

#13521

Depositor

Shelley Berger

Article

Huang et al Nature. 2006 Nov 30. 444(7119):629-32.

Insert

Smyd2 (Smyd2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
pCMV mouse Dscam-like

Plasmid

#18738

Depositor

Joshua Sanes

Article

Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

Insert

DscamL

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only
Lenti-sgRb1/Cre

Plasmid

#89647

Purpose

Expresses an Rb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Rb1 (Rb1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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PB-Hyg-Dest-mCherry-PAR-6B

Plasmid

#194908

Purpose

To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

PAR-6B (Pard6b Mouse)

Use

Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

39 C to G, silent mutation

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-DIO-mCReP

Plasmid

#167503

Purpose

Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

Ppp1r15b (Ppp1r15b Mouse)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

hSyn

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN3P-HA-Suv4-20h2

Plasmid

#86691

Purpose

For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)

Tags

HA

Promoter

T3

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-O-FUW-FoxG1

Plasmid

#35547

Depositor

Marius Wernig

Article

Lujan et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

FoxG1 (Foxg1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2K-p53DN-T2A-copGFP

Plasmid

#109229

Purpose

For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

dominant negative p53 (Trp53 Mouse)

Tags

copGFP

Expression

Mammalian

Mutation

dominant negative p53

Promoter

CAGGS

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CACNA1A KI

Plasmid

#131480

Purpose

Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TET-mcMyc

Plasmid

#20888

Purpose

piggyBac vector with tet inducible mcMyc

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

cMyc (Myc Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-mBrn2

Plasmid

#19711

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

mouse Brn2 (Pou3f2 Mouse)

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 mouse IL-6 (1240)

Plasmid

#66908

Purpose

Mammalian expression of IL-6

Depositor

Michael Karin

Insert

IL-6 (Il6 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-GFP-Rab27a

Plasmid

#194985

Purpose

overexpress Rab27a

Depositor

Angelique Bordey

Insert

GFP-Rab27a

Expression

Bacterial

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIN-EF1a-mNeonGreen-P2A-Cas9

Plasmid

#189807

Purpose

Retroviral delivery of Cas9

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Cas9

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

Plasmid

#86194

Purpose

beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

Depositor

Robert Singer

Article

Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

Insert

beta Globin (HBB, Hbb Mouse, Human)

Use

Tet on

Expression

Mammalian

Mutation

39TER in beta Globin in MCS II

Promoter

Tet on

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR (Puro) p65 shRNA1

Plasmid

#22507

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA1 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218633

Purpose

Proximity biotinylation of msNLRP3

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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12,913 results