Addgene

We narrowed to 16,036 results for: JUN

Showing: 3301 - 3350 of 16036 results

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mTagRFP-T-Paxillin-22

Plasmid

#58024

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLNCX Myr His p110 KD

Plasmid

#10837

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 KD (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Mutation

D915S. Kinase Dead.

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
TRPML3-CFP

Plasmid

#18834

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

Q165R, F210Y

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-RapR-p38

Plasmid

#25935

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-p38 (Mapk14 Mouse, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Apr)

Plasmid

#110078

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRJPaph-eBFP2

Plasmid

#67030

Purpose

Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Insert

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5 dOrai

Plasmid

#16368

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai (olf186-F Fly)

Use

Tags

V5

Expression

Insect

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pISI-mmP16 3' UTR

Plasmid

#22693

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mmP16 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pFA6a-TRP1-PGAL1-HBH

Plasmid

#26886

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag N-terminal

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Tublin/pcDNA3

Plasmid

#67780

Purpose

Tublin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

b-Tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
pEE390

Plasmid

#149280

Purpose

T-DNA encoding TRV2 with gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pILGFP3

Plasmid

#65451

Purpose

pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Use

Tags

yEGFP reporter

Expression

Yeast

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pITGFP3

Plasmid

#65452

Purpose

pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Use

Tags

yEGFP-CLN2PEST reporter

Expression

Yeast

Mutation

Promoter

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pAGM55351

Plasmid

#169092

Purpose

MoClo level 1 35S promoter-BvADHα-Nos terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvADHα-Nos terminator

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-RalA-V23

Plasmid

#15252

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed 23rd amino acid to valine

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pMEXC3GH (Apr)

Plasmid

#110083

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_Lyn11-STf

Plasmid

#182008

Purpose

CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lyn11-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

Plasmid

#185686

Purpose

Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-HaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRalA-2

Plasmid

#15258

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pGEX Smad3 C

Plasmid

#12634

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 C (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C segment of Smad3 (aa 199-425)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
MKK4

Plasmid

#29579

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK4 (MAP2K4 Human)

Use

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Tags

Expression

Yeast

Mutation

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Clathrin

Plasmid

#67773

Purpose

Clathrin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Clathrin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Use

Tags

HA

Expression

Plant

Mutation

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C-Ag15

Plasmid

#11868

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Use

Tags

His and myc

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pFAST NPC1(NTD)-3Q

Plasmid

#25772

Depositor

Johann Deisenhofer

Article

Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

Insert

npc1 (NPC1 Human)

Use

Tags

His

Expression

Insect

Mutation

aa23-252 only; N70Q N122Q N185Q

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pET15b-pknG

Plasmid

#102814

Purpose

Expression of PknG in E.coli

Depositor

Jean Pieters

Article

Walburger et al Science. 2004 Jun 18;304(5678):1800-4. E

Insert

pknG

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179077

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Kohinoor-Vimentin/pcDNA3

Plasmid

#67772

Purpose

Vimentin-targeted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor-Vimentin

Use

Tags

Vimentin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
C-Ag20(0)

Plasmid

#11866

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Use

Tags

His and myc

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
CMV-miR-31 sponge

Plasmid

#25025

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

CMV-miR-31 sponge

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pX330A_D10A-1x3

Plasmid

#58773

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
RV-Cag-Dio-mEYFP

Plasmid

#87666

Purpose

retroviral vector encoding cre dependent expression of membrane EYFP

Depositor

Oscar Marin, Lynette Lim

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-EYFP

Use

Retroviral

Tags

palmitylation

Expression

Mutation

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pECFP-Sec23A

Plasmid

#66610

Purpose

CFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
Kohinoor-H2B/pcDNA3

Plasmid

#67774

Purpose

Histon2B-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

H2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
cr3Dd

Plasmid

#184862

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, bet, exo

Use

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH579

Plasmid

#172163

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD JM10.1); AraC

Use

Tags

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

Plasmid

#178996

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZLblast-RalA WT(RalA 402i-resistant)

Plasmid

#11124

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalA WT (RALA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

resistant to siRNA 402i

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
C-Ag20(8)

Plasmid

#11867

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Use

Tags

His and myc

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

Plasmid

#178995

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE387

Plasmid

#149285

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_FT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmMe31B

Plasmid

#21683

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

maternal expression at 31B (me31B Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mOrange2-Paxillin-22

Plasmid

#57970

Purpose

Localization: Focal Adhesions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pDEST51-LRRK2-R1441H

Plasmid

#25083

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arg 1441 mutated to His

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pJAZ10::LUC

Plasmid

#141172

Purpose

Luminescent reporter for jasmonic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtJAZ10 promoter

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtJAZ10, AT5G13220

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmDCP2-V5His6

Plasmid

#22410

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

decapping protein 2, variant A (DCP2 Fly)

Use

Drosophila expression

Tags

EGFP and V5-His6

Expression

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge

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