161,252 results

1. ABE-RA3.0

   Plasmid

   #208301

   Purpose

   Expresses ABE-RA3.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGRB

   Plasmid

   #71539

   Purpose

   for expression of gRNA in E. coli

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J231196

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lyso-B

   Plasmid

   #209873

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PPRE X3-TK-luc

   Plasmid

   #1015

   Purpose

   PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

   Depositor

   Bruce Spiegelman

   Article

   Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

   Insert

   PPRE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4xmts-G-Ca-FLITS

   Plasmid

   #191462

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   4xmts

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TRE-EGFP-EF1a-rtTA

   Plasmid

   #104454

   Purpose

   PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

   Depositor

   Volker Busskamp

   Article

   Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

   Inserts

   enhanced green fluorescent protein
   reverse tetracycline-controlled transactivator 3
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-flag-GFP-iCAM-1

   Plasmid

   #205186

   Purpose

   This vector encodes of a synCAM (iCAM1) and GFP

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM iCAM1 and GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-FGE

   Plasmid

   #75147

   Purpose

   Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

   Depositor

   Richard Fishel

   Article

   Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

   Insert

   FGE
   

   Use

   Tags

   Histidine tag and Thrombin

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   T7

   Available since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pICH47742:: pRPS5a-PE2max-NC::tHSP

   Plasmid

   #227694

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by pRPS5a and terminated by tHSP
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pRPS5a

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+ heiCas9

    Plasmid

    #186929

    Purpose

    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

    Depositor

    Joachim Wittbrodt

    Article

    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

    Insert

    myc-flexible linker-oNLS-Cas9-oNLS
    

    Use

    CRISPR

    Tags

    myc-flexible linker-optimizedNLS and optimizedNLS

    Expression

    Mammalian

    Mutation

    Promoter

    sp6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1-YY1

    Plasmid

    #169018

    Purpose

    Expression of YY1 driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human YY1 (YY1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJet1.2 ermB cassette

    Plasmid

    #117121

    Purpose

    Erythromycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Erythromycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    David Root, William Hahn, John Doench

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTA7001-DEST

    Plasmid

    #71745

    Purpose

    GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GAL4

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-mCh-Cry2olig

    Plasmid

    #101222

    Purpose

    Cry2olig(E490G)

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2olig
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3HM1Luc4

    Plasmid

    #236277

    Purpose

    Mammalian expression of human muscarinic M1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    muscarinic M1 receptor (CHRM1 Human)
    

    Use

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF05J

    Plasmid

    #42941

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    Negative control Functionally Dead Human L1 element (L1RE1 Human)
    

    Use

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    ORF1 R261A, R262A

    Promoter

    Available since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5/FRT-HA-hM4D(Gi)

    Plasmid

    #45548

    Purpose

    Expresses hM4D (Gi) neuronal inhibitor

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM4D (Gi)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFR_HCV_xb

    Plasmid

    #11510

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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21. mRFP-Rab5

    Plasmid

    #14437

    Purpose

    Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 5 (RAB5A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLV-EF1a-IRES-Hygro

    Plasmid

    #85134

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AP2u2-mCherry

    Plasmid

    #27672

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Ap2m1 (Ap2m1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBeloBac11loxP2272

    Plasmid

    #60342

    Purpose

    This is a BAC vector that contains both wt and mutant loxP sites.

    Depositor

    Jon Coren

    Article

    Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBPhsFlp1

    Plasmid

    #32148

    Purpose

    Expresses FLP recombinase in Drosophila

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    hsFlp1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Aspartic Acid at Amino Acid 5

    Promoter

    Hsp70Bb promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSpCTRE-CD4

    Plasmid

    #114010

    Purpose

    Dox-inducible Cas9 expression with a CD4 selection marker

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB-EF1a-TetOn3G

    Plasmid

    #104543

    Purpose

    PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    Tet-On 3G
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TRE:GFP

    Plasmid

    #163036

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pRSET iGluSnFR3 v857

    Plasmid

    #175186

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v857
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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