167,150 results
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Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
TagsHisExpressionBacterialMutationWt geneAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH
Plasmid#86470PurposeYeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoterDepositorTypeEmpty backboneTagsHIS10-TEV-HAExpressionYeastPromoterGAL1Available SinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4-ERSE1-luc2P-Hygro
Plasmid#101789PurposeLuferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)DepositorInsert3xERSE-I
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-BAZ1A
Plasmid#65371PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertBAZ1A (BAZ1A Human)
TagsEmGFP and V5ExpressionMammalianMutation796I compared to all reference sequencesPromoterCMVAvailable SinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-pcDNA3.1 Puro-CAG-Voltron2
Plasmid#172909PurposeMammalian expression of voltage sensorDepositorInsertVoltron2
ExpressionMammalianMutationA122DPromoterCAGAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
Tags8 histidine tagExpressionBacterialPromoterT7 promotorAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF0815
Plasmid#141717PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Tmem192-2xFlag
Plasmid#102929PurposeLentiviral expression of a lysosomal tag (Tmem192-Flag)DepositorAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-NLS-Cas9-6xHis
Plasmid#62934PurposeExpression of NLS-Cas9-6xHis in bacterial cellsDepositorInsertNLS-Cas9-6xHis
Tags6xHis tag and NLSExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lact-C2-GFP
Plasmid#22852DepositorAvailable SinceJan. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmVen-Treslin
Plasmid#211405Purposemammalian expression of human Treslin N-terminally fused to mVenusDepositorAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE
Plasmid#162375PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman U6Available SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GCaMP6s
Plasmid#171156PurposeEncodes GCaMP6sDepositorInsertGCaMP6s
ExpressionMammalianAvailable SinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone
Plasmid#216154PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic exon and C-terminal FLAG
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry
Plasmid#193465PurposeDn29 Donor with promoter for mcherry and puro expressionDepositorInsertDn29_attP
ExpressionMammalianPromoterEf1aAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-mCh
Plasmid#129591PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
ExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA
Plasmid#163476PurposeDirect-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpADepositorInsertiCre
UseAAVPromoterBeta Globin minimal promoterAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-ASAP5-WPRE
Plasmid#225709PurposePan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imagingDepositorInsertASAP5
UseAAVPromoterhuman synapsinAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIP-Flag-TfebAA
Plasmid#79014PurposeFor mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.DepositorAvailable SinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EPHA3
Plasmid#116733PurposeLentiviral expression of EPHA3DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-cOVA-IRES-BFP
Plasmid#135074PurposeExpresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.DepositorAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG
Plasmid#241423PurposeProtein Transposition Cassette Containing CatN and CfaCDepositorInsertHis5-Sumo-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG
TagsHis5, HAExpressionBacterialAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFP/Cre
Plasmid#49056PurposeAdeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.DepositorInsertCre
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP, Myc, and NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863AAvailable SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-humanTRAF6 (1093)
Plasmid#66929PurposeMammalian Expression of TRAF6DepositorAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt3
Plasmid#66832PurposeMammalian Expression, Bacterial ExpressionDepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEZT-BM
Plasmid#74099PurposeMulti-purpose rapid titer bacmam expression vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseBacmam, baculovirusTagsEGFP following p10 promoterExpressionMammalianPromoterCMV, p10Available SinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GCaMP6s.WPRE.SV40
Plasmid#100844PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only