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Plasmid#170255PurposeBarcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertSV40 Large T Antigen
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorInsertMLL-Af9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mito-7
Plasmid#55102PurposeLocalization: Mitochondria, Excitation: 587, Emission: 610DepositorInsertMito (COX8A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) eGFP
Plasmid#129020PurposeExpresses eGFPDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_puro optimized backbone
Plasmid#73797Purposeoptimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hSK
Plasmid#27078PurposeIntegration-free (episomal) expression of human SOX2 and KLF4DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP-LC3ΔG
Plasmid#84572PurposeExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI30
Plasmid#183749PurposeAAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.DepositorInsertAAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
UseAAVTagsExpressionMutationK449R, and NNSTRGG inserted between 588 and 589 o…Promoterp41Available sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET246-pUC57 12xMS2V6
Plasmid#104390PurposeMS2V6 cloningDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1-GFP
Plasmid#51407PurposeIs a biosensor for PI(4,5)P2DepositorInsertPH-PLCD1 (aa 1–170) (PLCD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…TagsExpressionMutationP697S mutation in putative replicase. S239G and S…PromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-S-tdTomato
Plasmid#112579PurposeExpressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vectorDepositorInsertNLS-tdTomato-NES
UseLentiviralTagsnuclear export sequence and nuclear localization …ExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-mitoDsRed
Plasmid#44386Purpose3rd generation lentiviral vectorDepositorInsertmitoDsRed
UseLentiviralTags61 aa targeting sequence of the P1 isoform F1F0-A…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-IRES-Neo
Plasmid#28019DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-Blast
Plasmid#83481PurposeLentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTADepositorInsertsSpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterTight Tre and human phosphoglycerate kinase 1 pro…Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-myc-GLUT4-mCherry
Plasmid#64049Purpose3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocationDepositorInsertGLUT4 (Slc2a4 Mouse)
UseLentiviralTagsc-Myc and mCherryExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only