165,567 results

1. pRC0940 (circRNA entry vector)

   Plasmid

   #189967

   Purpose

   Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. OpenCRISPR-1

   Plasmid

   #221565

   Purpose

   Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-CAG-GFP

   Plasmid

   #37825

   Purpose

   AAV-mediated expression of GFP under the CAG promoter

   Depositor

   Edward Boyden

   Has Service

   AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

   Insert

   GFP
   

   Use

   AAV; Adeno-associated virus

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-Cas3

   Plasmid

   #209427

   Purpose

   Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

   Depositor

   Tilmann Weber

   Article

   Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

   Insert

   codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PtipA; crRNA under control of PermE*

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SBE4-Luc

   Plasmid

   #16495

   Purpose

   Luciferase reporter containing four copies of the Smad binding site

   Depositor

   Bert Vogelstein

   Article

   Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

   Insert

   Smad binding element
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-GRAB-rDA3m

   Plasmid

   #208702

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3m
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-PPARA

   Plasmid

   #169019

   Purpose

   Expression of PPARA driven by CMV promoter

   Depositor

   Claes Wadelius

   Article

   Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

   Insert

   human ppara (PPARA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIX-hN1ICD

   Plasmid

   #91897

   Purpose

   Doxycycline inducible expression of human Notch1 ICD

   Depositor

   Julien Sage

   Insert

   Notch1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   codons 1770 to 2555 of human NOTCH1

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-MS2-HA-HsSmg5_T

   Plasmid

   #147552

   Purpose

   Mammalian Expression of HsSmg5

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5 (SMG5 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAF0036 Ef1a-Si74-2a-EGFP

    Plasmid

    #193463

    Purpose

    Expression of Si74 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid

    #179105

    Purpose

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRC0569 (parts entry vector)

    Plasmid

    #189966

    Purpose

    Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    BBa-J23100

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. L4440

    Plasmid

    #1654

    Depositor

    Andrew Fire

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, T7p, lacZN, OriF1>>, OriF1<<

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Myc-TEAD4

    Plasmid

    #24638

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    TEAD-4 (TEAD4 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSI-Check2-hRluc-NFkB-firefly

    Plasmid

    #106979

    Purpose

    Luciferase reporter with internal control for NFkB signal.

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    NFKB recognition sites (NFKB1 Human)
    

    Use

    Luciferase

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH47751

    Plasmid

    #48002

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

    Plasmid

    #203348

    Purpose

    Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

    Depositor

    Christopher Grigsby, Molly Stevens

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Template for t7-mediated in vitro transcription o…

    Tags

    Flanked by 5' and 3' UTRs from Xenopus …

    Promoter

    T7

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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