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Showing: 181 - 210 of 157134 results

  3. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Use
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  9. psPAX2-D64V

    Plasmid
    #63586
    Purpose
    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.
    Depositor
    David Rawlings, Andrew Scharenberg
    Article
    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi
    Insert
    Packaging plasmid for integrase deficient lentiviral vectors
    Use
    Integrase deficient lentiviral packaging vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    63586-plasmid-map-sequence-id-464218

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  12. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234436
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234436-plasmid-map-sequence-id-465719

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  18. eeBxb1_IVT

    Plasmid
    #222342
    Purpose
    Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template
    Depositor
    David Liu
    Article
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    Template for in vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7 (inactivated)
    Available since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222342-plasmid-map-sequence-id-440249

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  19. FH-Tet2-pEF

    Plasmid
    #41710
    Purpose
    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system
    Depositor
    Anjana Rao
    Article
    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi
    Insert
    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    Use
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    41710-plasmid-map-sequence-id-322448

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  20. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    James Zhe Liu
    Article
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91564-plasmid-map-sequence-id-180643

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  21. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

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  23. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid
    #216156
    Purpose
    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216156-plasmid-map-sequence-id-437768

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  24. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Jerry Crabtree
    Article
    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    IL-2 promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    17870-plasmid-map-sequence-id-325133

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  30. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid
    #65727
    Purpose
    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.
    Depositor
    Jacco van Rheenen
    Article
    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1
    Inserts
    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and UBC
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    65727-plasmid-map-sequence-id-243566

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Showing: 181 - 210 of 157134 results