159,238 results
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Plasmid#222105PurposeA ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.DepositorInsertvirA
UseTagsExpressionBacterial and PlantMutationPromotervirA promoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD
Plasmid#98669PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43DepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX_DSB_Spectrum-V1
Plasmid#192474PurposeLentiviral DSB Spectrum V1 Reporter SystemDepositorInsertBFP1.2, incompleteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-DN KASH
Plasmid#125553Purposemcherry labeled DN-KASHDepositorInsertSYNE1 (SYNE1 Human)
UseTagsmcherryExpressionMammalianMutationTruncated form: only the KASH domain of nesprin-…PromoterCMVAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
hFerritinH
Plasmid#122652PurposeExpression of hFerritinH in bacterial cellsDepositorInsertferritin heavy chain 1 (FTH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VAPB
Plasmid#108126PurposeER markerDepositorInsertmCherry:VAPB (VAPB Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVL
Plasmid#64085PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain polymerase geneDepositorInsertVSV polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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PGK-H2BmCherry
Plasmid#21217DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-mCherryExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
Mito-GA
Plasmid#209865PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP A
UseTagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucOS
Plasmid#22777DepositorInsertOVA-SIY
UseCre/Lox and LentiviralTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFB2C
Plasmid#195285PurposeBicistronic HSV-TK and Hygromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Hygro
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone
Plasmid#216153PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic splice site and C-terminal FLAG tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-mcherry
Plasmid#161974PurposeExpresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNADepositorInsertCas9-t2a-mcherry
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
polyQ74-GFP
Plasmid#231893PurposeForms cytosolic HTT partial exon 1 Q74 aggregatesDepositorInsertHTT partial exon 1 Q74 (HTT Human)
UseTagsEGFPExpressionMammalianMutationCAG repeat expansionPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP1LC3B
Plasmid#137757PurposeExpression of LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
LD-GA
Plasmid#209862PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP A
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMKV057
Plasmid#133312PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPTDepositorInsertLuc+. WUS2, IPT
UseTagsExpressionPlantMutationPromoterAtUbi10, Nos, 35SAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
mAG-GAL3
Plasmid#62734PurposeMammalian vesicle leakage reporterDepositorInsertmAG-Galectin 3
UseLentiviralTagsmAzami-GreenExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_PLCG1:1-1291
Plasmid#211089PurposeMammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.DepositorInsertPLCG1:M1-L1291 (PLCG1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationisoform 2. I813T natural variant (VAR_011908)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4,5)P2 Probe
Plasmid#211513PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteriaDepositorInsertPLCdelta1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAF0033 Ef1a-Pa01-2a-EGFP
Plasmid#193460PurposeExpression of RecombinaseDepositorInsertPa01
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4)P Probe
Plasmid#211509PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteriaDepositorInsertSidC (P4C)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(Hygro) myc-hPolQ-Flag
Plasmid#73132PurposeTo express in mammalian cells human PolQDepositorInsertPolymerase theta (POLQ Human)
UseTagsFlag and mycExpressionMammalianMutationPromotercmv promoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only