163,404 results

1. Mitochondrial PancACe

   Plasmid

   #215710

   Purpose

   Expresses acetyl-CoA biosensor in the mitochondria

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and mitochondrial localization sequence…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. px459V2.0_Conc2

   Plasmid

   #134451

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA-3xHA-hTERT

   Plasmid

   #51637

   Purpose

   Mammalian expression of 3xHA tagged human TERT, N-terminal.

   Depositor

   Steven Artandi

   Article

   Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

   Insert

   TERT (TERT Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. evoBxb1

   Plasmid

   #222338

   Purpose

   Plasmid for expressing evolved Bxb1 in the mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBITE-CD19-CD3

   Plasmid

   #190677

   Purpose

   Encodes a modularized form of HD37-OKT3 bispecific antibody

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Modularized-HD37-modularized-OKT3-P2A-NeonGreen
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-BRD4

   Plasmid

   #65378

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   R1232W

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

   Plasmid

   #44362

   Purpose

   Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cytoplasmic PancACe

   Plasmid

   #215708

   Purpose

   Expresses acetyl-CoA biosensor in the cytoplasm

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and cytoplasmic localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of AA 1 to 45

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX_lenti_osTir1_9Myc_P2A_Bsd

    Plasmid

    #139746

    Purpose

    lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    rice E3 ligase Tir1 (osTir1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mU6-sgRNA-CLTA-mCherry

    Plasmid

    #240637

    Purpose

    Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    puro-T2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nuclear PancACe

    Plasmid

    #215706

    Purpose

    Expresses acetyl-CoA biosensor in the nucleus

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSGV1-1D3-28Z.1-3 mut

    Plasmid

    #107227

    Purpose

    Anti-mouse CD19 CAR with CD3 ITAMs mutated

    Depositor

    Steven Rosenberg, James Kochenderfer

    Article

    Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

    Insert

    1D3-28Z. 1-3 mut
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    None

    Promoter

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cavb3

    Plasmid

    #26574

    Depositor

    Diane Lipscombe

    Insert

    calcium channel beta subunit-III (Cacnb3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-CD14

    Plasmid

    #13645

    Depositor

    Doug Golenbock

    Insert

    CD14 (CD14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW

    Plasmid

    #14882

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PIEZO1-HaloTag

    Plasmid

    #207834

    Purpose

    PIEZO1 fusion for biochemistry and live imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    PIEZO1 (PIEZO1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPSU150

    Plasmid

    #173836

    Purpose

    Expresses 150 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3 MDM2 WT

    Plasmid

    #16233

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-FLAG-LKB1

    Plasmid

    #8590

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEB2-mScarlet-I

    Plasmid

    #104007

    Purpose

    Plasmid encoding mScarlet-I

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet-I
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lact-C2-GFP

    Plasmid

    #22852

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 Domain (MFGE8 Bovine)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENN496_miniCRISPRcharm_dSaCas9

    Plasmid

    #220837

    Purpose

    mini CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    mini CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Pet28a_Human_H3.1

    Plasmid

    #42631

    Depositor

    Joe Landry

    Insert

    Human H3.1 (H3C1 Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid

    #85165

    Purpose

    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

    Depositor

    Robin Dowell

    Article

    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

    Insert

    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    

    Use

    Tags

    AmilCP and RTX

    Expression

    Bacterial

    Mutation

    Promoter

    J23100 promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lenti-tmpknot-epegRNA-puro backbone

    Plasmid

    #214089

    Purpose

    Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-FLAG-KD LKB1

    Plasmid

    #8591

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K78I: lysine 78 mutated to isoleucine; kinase dead

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge