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Showing: 121 - 140 of 77439 results

  1. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid
    #26429
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26429-plasmid-map-sequence-id-354259

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  8. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  9. pLKO.3G

    Plasmid
    #14748
    Depositor
    Christophe Benoist, Diane Mathis
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14748-plasmid-map-sequence-id-352429

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  10. pCas

    Plasmid
    #62225
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Depositor
    Sheng Yang
    Article
    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii
    Has Service
    Cloning Grade DNA
    Insert
    cas9
    Use
    CRISPR; Lambda red recombinase
    Expression
    Bacterial
    Promoter
    native cas9 promoter
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    62225-plasmid-map-sequence-id-275267

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  11. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available Since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  14. Lamp1-RFP

    Plasmid
    #1817
    Purpose
    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Available Since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    1817-plasmid-map-sequence-id-350820

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  19. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-472945

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  20. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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Showing: 121 - 140 of 77439 results