Skip to main content
Addgene
Showing: 3451 - 3500 of 9510 results

  3. pcDNA3.1-Flag-gp130dimer

    Plasmid
    #174394
    Purpose
    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).
    Depositor
    Dennis Ko
    Article
    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-
    Insert
    IL6ST cytosolic domain with leucine zipper & C-C linker
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174394-plasmid-map-sequence-id-341883

    Enlarge

  15. pCS2 GFP-GSK3-MAPK

    Plasmid
    #29689
    Purpose
    GFP reporter protein fused to three GSK3 phosphorylation sites
    Depositor
    Edward De Robertis
    Article
    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.
    Insert
    GFP glycogen synthase kinase 3 recognition site primed by MAPK
    Use
    Tags
    Flag and Flag-EGFP
    Expression
    Mammalian
    Mutation
    recognition sites only, and E3 ligase site
    Promoter
    SP6
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29689-plasmid-map-sequence-id-127238

    Enlarge

  33. pCS2 GFP-GSK3mut-MAPK

    Plasmid
    #29690
    Purpose
    GFP reporter protein fused to three mutated GSK3 phosphorylation sites
    Depositor
    Edward De Robertis
    Article
    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.
    Insert
    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    Use
    Tags
    Flag and Flag-EGFP
    Expression
    Mammalian
    Mutation
    recognition sites only, and E3 ligase site, GSK3 …
    Promoter
    SP6
    Available since
    Jan. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    29690-plasmid-map-sequence-id-129831

    Enlarge

  35. pcDNA3-ER-CaNAR2

    Plasmid
    #64732
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal 27 amino acids from CYP450,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64732-plasmid-map-sequence-id-109351

    Enlarge

  43. pcDNA3-PM-CaNAR2

    Plasmid
    #64730
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    Lyn-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal targeting sequence from Lyn…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64730-plasmid-map-sequence-id-109348

    Enlarge

  47. pME-mKate2-KDEL-pA

    Plasmid
    #109546
    Purpose
    Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109546-plasmid-map-sequence-id-223868

    Enlarge

Showing: 3451 - 3500 of 9510 results