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pGOAL19

Plasmid

#20190

Depositor

Tanya Parish

Article

Parish et al Microbiology (Reading). 2000 Aug;146 ( P

Type

Empty backbone

Use

Marker cassette

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP

Plasmid

#21373

Purpose

self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

Plasmid

#102796

Purpose

Retrovirus for delivery of one sgRNA (empty, bbsI)

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

U6 sgRNA

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Plenti-CMV-MCS-RFP-SV-puro

Plasmid

#109377

Purpose

This plasmid expresses dsRed2 and puromycin resistance

Depositor

Jonathan Garlick, Behzad Gerami-Naini

Type

Empty backbone

Use

Lentiviral

Tags

dsRed2

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau E14

Plasmid

#46907

Purpose

expresses EGFP tagged Tau E14 in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau E14 (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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Human MAC-1 alpha

Plasmid

#8631

Depositor

Timothy Springer

Article

Corbi et al J Biol Chem 1988 Sep 5;263(25):12403-11.

Insert

MAC-1 alpha (ITGAM Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
AB-GFP

Plasmid

#72203

Purpose

screen AB42 variants for aggregation

Depositor

Michael Hecht

Article

Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

Insert

AB42 (APP Human)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRDA_550

Plasmid

#203398

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a

Depositor

John Doench

Article

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Insert

Enhanced Acidaminococcus Cas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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NW30

Plasmid

#133444

Purpose

Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW30

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Article

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-GFP

Plasmid

#67634

Purpose

AAV vector expressing GFP

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

GFP

Use

AAV

Promoter

CMV

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD14

Plasmid

#13645

Depositor

Doug Golenbock

Insert

CD14 (CD14 Human)

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-human OCRL

Plasmid

#22207

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Oculocerebrorenal syndrome of Lowe (OCRL Human)

Use

Retroviral

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-YFP

Plasmid

#13033

Purpose

Mammalian expression vector for cloning your gene fused to YFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

Yellow fluorescent protein

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ER alpha

Plasmid

#28230

Purpose

Mammalian expression of Estrogen Receptor alpha fused to EGFP

Depositor

Michael Mancini

Article

Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

Insert

Estrogen Receptor alpha (ESR1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pMSCV-Zeo

Plasmid

#75088

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-SIRT1

Plasmid

#1791

Purpose

Mammalian expression of flag-tagged SIRT1

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-EF1a-YapS127A

Plasmid

#46049

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

Flag tag

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pLenti6.2_mTagBFP2

Plasmid

#113725

Purpose

Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

Depositor

Vanessa LaPointe

Article

Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

Insert

mTagBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR3-CFP

Plasmid

#13641

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 3 (TLR3 Human)

Tags

CFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

Plasmid

#118593

Purpose

Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha/TRE3G

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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CaV1.2

Plasmid

#26572

Depositor

Diane Lipscombe

Article

Helton et al J Neurosci. 2005 Nov 2. 25(44):10247-51.

Insert

Calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c Mouse)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAGM4723

Plasmid

#48015

Purpose

Please see depositor comments section.

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has Service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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myc-mTOR

Plasmid

#1861

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

mTOR (Mtor Rat)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pEJS578_DD-dSpyCas9-mCherry-APEX2

Plasmid

#108570

Purpose

Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Insert

dSpyCas9

Use

CRISPR and Lentiviral

Tags

APEX2, Destabilization domains, Flag, and mCherry…

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-hCD19

Plasmid

#127889

Purpose

Expresses hCD19

Depositor

Anjana Rao

Article

Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

Insert

hCD19 (CD19 Human)

Use

Retroviral

Tags

no tags

Mutation

none

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR

Plasmid

#171240

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor (AR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Article

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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