165,775 results

1. pcDNA3-FlipCherry-T2A-GFP

   Plasmid

   #124436

   Purpose

   Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipCherry-T2A-GFP
   

   Expression

   Mammalian

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCNICK

   Plasmid

   #84653

   Purpose

   Genome editing for Lactobacillus casei Lc2W

   Depositor

   Sheng Yang

   Article

   Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

   Inserts

   Cas9 nickase
   sgRNA
   homology arms of LC2W_2179
   

   Use

   CRISPR

   Mutation

   D10A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-eNme2-C-ABE8e

   Plasmid

   #185667

   Purpose

   Expresses eNme2-C-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-C
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHDM-Hgpm2

   Plasmid

   #164441

   Purpose

   Encodes codon-optimized HIV gagpol for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   Codon Optimized HIV-1 GagPol
   

   Expression

   Mammalian

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRE recombinase

   Plasmid

   #62730

   Purpose

   Expresses Cre recombinase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry Vinculin

   Plasmid

   #50527

   Depositor

   Kenneth Yamada

   Insert

   Vinculin (VCL Chicken)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-hMLH1dn for IVT

   Plasmid

   #178114

   Purpose

   Template for in vitro transcription of human MLH1dn

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (codon optimized) (MLH1 Human)
   

   Use

   Template for in vitro transcription

   Mutation

   Detailed in manuscript

   Promoter

   T7 (inactivated)

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Butelase AY

   Plasmid

   #221847

   Purpose

   Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

   Insert

   Butelase 1 ligase
   

   Expression

   Bacterial

   Mutation

   Val237Ala, Thr238Tyr

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH41331::pU6cm-pegR::EURb7

    Plasmid

    #227695

    Purpose

    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6 composite

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mRuby3-C1

    Plasmid

    #127808

    Purpose

    mRuby3 C1 Cloning vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    mRuby3
    

    Expression

    Mammalian

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-MCS1-P2A-MCS2-mDux

    Plasmid

    #138320

    Purpose

    Dox-inducible codon-optimized mouse Dux

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    Dux (Dux Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MS2-HB

    Plasmid

    #35573

    Depositor

    Marian Waterman

    Article

    Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

    Insert

    MS2 Coat Protein (cp Bacteriophage)
    

    Use

    Retroviral

    Tags

    HTBH

    Expression

    Mammalian

    Mutation

    Insert is a tail-to-head tandem placement of two …

    Promoter

    CMV

    Available Since

    May 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRlsinPGK_H2BGFP_WPRE

    Plasmid

    #91788

    Purpose

    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHM129

    Plasmid

    #225534

    Purpose

    Encodes destablized mCherry under AR8 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    mCherry
    BFP
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBItetDT960GFP

    Plasmid

    #80419

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 960 interrupted CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167937

    Purpose

    Inducible CRISPRa expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    dxCas9(3.7)-VPR
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

    Plasmid

    #223712

    Purpose

    Expresses autophagy flux LC3B fluorescence reporter

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    LC3B (MAP1LC3B Synthetic, Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    mCherry::EGFP::LC3B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJMP1

    Plasmid

    #79873

    Purpose

    Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

    Depositor

    Carol Gross, Jason Peters, Stanley Qi

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    xylA

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-beta-3

    Plasmid

    #27289

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin beta 3 (ITGB3 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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