161,252 results

1. pcDNA3.1-Opa1

   Plasmid

   #191963

   Purpose

   Expression of OPA1.

   Depositor

   Luca Scorrano

   Insert

   Opa1 (Opa1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mNeonGreen-Sec31A

   Plasmid

   #226582

   Purpose

   Encodes Sec31A protein labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

   Insert

   Sec31A (Protein transport protein) (SEC31A Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CBE-T1.14

   Plasmid

   #193277

   Purpose

   Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.14
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYU-2-Env

   Plasmid

   #133997

   Purpose

   Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

   Depositor

   Himanshu Garg

   Article

   Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

   Insert

   Envelope Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA Display H3_A/Hong Kong/1/1968

   Plasmid

   #206506

   Purpose

   Expresses H3_Hong Kong/1/1968 in the surface of mammalian cells

   Depositor

   IIya Finkelstein

   Insert

   H3_A/Hong Kong/1/1968
   

   Use

   Tags

   3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

   Expression

   Mammalian

   Mutation

   deleted amino acids 1651 to 1735

   Promoter

   CMV

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTnos (GB0037)

   Plasmid

   #68188

   Purpose

   Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Nos terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-flag-GFP-Intb1

   Plasmid

   #205193

   Purpose

   This vector encodes of a synCAM (Intb1) and GFP

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM Intb1 and GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-Rncp-iGluSnFR1

   Plasmid

   #107336

   Purpose

   Red fluorescent glutamate sensor with ncp topology. Membrane-displayed

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

   Insert

   Rncp-iGluSnFR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJet1.2 cat cassette

   Plasmid

   #117119

   Purpose

   Chloramphenicol resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Chloramphenicol resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Calnexin-HT8-mGold

    Plasmid

    #183989

    Purpose

    Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    HaloTag8 (C61S, C262S), mGold

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-FLEX-SaCas9-U6-sgGrin2b

    Plasmid

    #124851

    Purpose

    Mutagenesis of Grin2b

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2b (Grin2b Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BE4-SsAPOBEC3B

    Plasmid

    #138343

    Purpose

    Mammalian expression plasmid for BE4-SsAPOBEC3B

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-SsAPOBEC3B
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR.CMV

    Plasmid

    #32688

    Purpose

    Mammalian expression and gateway entry vector

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Frt-V5-hspB8

    Plasmid

    #63109

    Purpose

    Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb8 (HSPB8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR- flag-Lag16-Ecad

    Plasmid

    #205197

    Purpose

    This vector encodes of the synCAM (Ecad) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Ecad and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-APX(W41F)-NES

    Plasmid

    #42608

    Depositor

    Alice Ting

    Article

    Rhee et al Science. 2013 Jan 31.

    Insert

    soybean APX W41F (dimeric) -NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    W41F mutation

    Promoter

    CMV

    Available since

    March 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-VPS15-TSF

    Plasmid

    #203563

    Purpose

    Mammalian expression of VPS15 with TwinStrep-Flag tag

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    VPS15 (PIK3R4 Human)
    

    Use

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEYFP-N1-HsSmg6_H

    Plasmid

    #146475

    Purpose

    Mammalian Expression of HsSmg6

    Depositor

    Elisa Izaurralde

    Article

    Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

    Insert

    HsSmg6 (SMG6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations T594C, A699G and two non sil…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWZL Neo Myr Flag ILK

    Plasmid

    #20505

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    ILK (ILK Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pHR-flag-GFP-JAM-B

    Plasmid

    #205191

    Purpose

    This vector encodes of a synCAM (JAM-B) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM JAM-B and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CAG-AausFP1-Z1

    Plasmid

    #135047

    Purpose

    AausFP1 - bright green fluorescent protein expression from A. australis under CAG promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    AausFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLC-HA-Nedd8-P2A-Hygro

    Plasmid

    #124306

    Purpose

    Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    Nedd8 (NEDD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGilesSHdI_psmyc_His6_triple red

    Plasmid

    #170047

    Purpose

    Mono-Responsive Reporter Gene Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z

    Plasmid

    #148113

    Purpose

    Mammalian Expression of HshnRNPA2-B1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPA2-B1 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    splicing variant of hnRNPA2 variant B1. Exon 6 an…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSF11-wmiRFP713-T2A-HO1

    Plasmid

    #197246

    Purpose

    Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP713-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. (114) pcDNA3.1-GFP-Flag-JunB-EPEA

    Plasmid

    #160743

    Purpose

    Expresses a GFP tagged JunB with Flag and EPEA tags.

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    GFP-Flag-JunB-EPEA (JUN Human)
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p11d-sctRPA

    Plasmid

    #102614

    Purpose

    Expression of S. cerevisiea RPA in E.coli

    Depositor

    Marc Wold

    Article

    Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

    Inserts

    RPA1 (RFA1 Budding Yeast)
    RPA2 (RFA2 Budding Yeast)
    RPA3 (RFA3 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RFA2 has intron deleted

    Promoter

    T7

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-flag-GFP-MUC4

    Plasmid

    #205189

    Purpose

    This vector encodes of a synCAM (MUC4) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM MUC4 and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Frt-hspB5

    Plasmid

    #63096

    Purpose

    Expression of HSPB5 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb5 (CRYAB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. VRK1_HUMAN_D0

    Plasmid

    #79684

    Purpose

    This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only