4,098 results

1. IRES-mCherry

   Plasmid

   #80139

   Purpose

   empty retroviral vector with IRES-mCherry

   Depositor

   Ellen Rothenberg

   Article

   Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV-dTomato

   Plasmid

   #21374

   Purpose

   self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato

   Depositor

   Bryan Welm

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas13d-dsRBD-APEX2

   Plasmid

   #154939

   Purpose

   TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA Flag-RARalpha

   Plasmid

   #35555

   Depositor

   Jonathan Kurie, Harish Srinivas

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR1A-GFP-N2 (FR1)

   Plasmid

   #19364

   Purpose

   Gateway entry vector for C-term eGFP fusion, frame N2

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV/TO Neo empty (w215-1)

   Plasmid

   #17485

   Purpose

   3rd gen lentiviral empty control vector, CMV/TO promoter, Neo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCSDest

   Plasmid

   #22423

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-EGFP-Cdc42(wt)

   Plasmid

   #12599

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (WT) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET His6 LIC cloning vector (2Bc-T)

   Plasmid

   #37236

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-Nanog

    Plasmid

    #13354

    Purpose

    Retroviral expression of mouse Nanog

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57-MCS1-P2A-MCS2 (GFP)

    Plasmid

    #80924

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-TFR-20

    Plasmid

    #55144

    Purpose

    Localization: Transferrin Receptor, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TFR (TFRC Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

    Plasmid

    #141386

    Purpose

    Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-M (M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. HA-cdc20

    Plasmid

    #11594

    Depositor

    Marc Kirschner

    Article

    Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

    Insert

    cdc20 (CDC20 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDestTol2CG2-U6:gRNA

    Plasmid

    #63156

    Purpose

    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWZL hygro H-Ras V12

    Plasmid

    #18749

    Depositor

    Scott Lowe

    Article

    Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

    Insert

    H-Ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V12

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR4-HaloTag (w876-1)

    Plasmid

    #29644

    Depositor

    Eric Campeau

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCW57-GFP-2A-MCS

    Plasmid

    #71783

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXs-puro GFP-p62

    Plasmid

    #38277

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSK+KanaRpsL

    Plasmid

    #20871

    Depositor

    Jiyue Zhu

    Article

    Wang et al Mol Biotechnol. 2009 Jan 22. ():.

    Insert

    KanamycinResistance-RpsL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBPGAL4.2Uw-2

    Plasmid

    #26227

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO5.sgRNA.EFS.tRFP

    Plasmid

    #57823

    Purpose

    3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLL3.7

    Plasmid

    #11795

    Purpose

    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

    Depositor

    Luk Parijs

    Article

    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCIP-Flag-TfebAA

    Plasmid

    #79014

    Purpose

    For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Tfeb (Tfeb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S142A, S211A

    Promoter

    pCAGGs

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EBFP2-C1

    Plasmid

    #54665

    Purpose

    Localization: C1 Cloning Vector, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge